TBCD links centriologenesis, spindle microtubule dynamics, and midbody abscission in human cells.

Microtubule-organizing centers recruit alpha- and beta-tubulin polypeptides for microtubule nucleation. Tubulin synthesis is complex, requiring five specific cofactors, designated tubulin cofactors (TBCs) A-E, which contribute to various aspects of microtubule dynamics in vivo. Here, we show that tu...

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Autores principales:	Mónica López Fanarraga, Javier Bellido, Cristina Jaén, Juan Carlos Villegas, Juan Carlos Zabala
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f1fe0b63fff84631a5a5d7f6373dbfd0
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spelling	oai:doaj.org-article:f1fe0b63fff84631a5a5d7f6373dbfd02021-11-25T06:26:27ZTBCD links centriologenesis, spindle microtubule dynamics, and midbody abscission in human cells.1932-620310.1371/journal.pone.0008846https://doaj.org/article/f1fe0b63fff84631a5a5d7f6373dbfd02010-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20107510/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Microtubule-organizing centers recruit alpha- and beta-tubulin polypeptides for microtubule nucleation. Tubulin synthesis is complex, requiring five specific cofactors, designated tubulin cofactors (TBCs) A-E, which contribute to various aspects of microtubule dynamics in vivo. Here, we show that tubulin cofactor D (TBCD) is concentrated at the centrosome and midbody, where it participates in centriologenesis, spindle organization, and cell abscission. TBCD exhibits a cell-cycle-specific pattern, localizing on the daughter centriole at G1 and on procentrioles by S, and disappearing from older centrioles at telophase as the protein is recruited to the midbody. Our data show that TBCD overexpression results in microtubule release from the centrosome and G1 arrest, whereas its depletion produces mitotic aberrations and incomplete microtubule retraction at the midbody during cytokinesis. TBCD is recruited to the centriole replication site at the onset of the centrosome duplication cycle. A role in centriologenesis is further supported in differentiating ciliated cells, where TBCD is organized into "centriolar rosettes". These data suggest that TBCD participates in both canonical and de novo centriolar assembly pathways.Mónica López FanarragaJavier BellidoCristina JaénJuan Carlos VillegasJuan Carlos ZabalaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 1, p e8846 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mónica López Fanarraga Javier Bellido Cristina Jaén Juan Carlos Villegas Juan Carlos Zabala TBCD links centriologenesis, spindle microtubule dynamics, and midbody abscission in human cells.
description	Microtubule-organizing centers recruit alpha- and beta-tubulin polypeptides for microtubule nucleation. Tubulin synthesis is complex, requiring five specific cofactors, designated tubulin cofactors (TBCs) A-E, which contribute to various aspects of microtubule dynamics in vivo. Here, we show that tubulin cofactor D (TBCD) is concentrated at the centrosome and midbody, where it participates in centriologenesis, spindle organization, and cell abscission. TBCD exhibits a cell-cycle-specific pattern, localizing on the daughter centriole at G1 and on procentrioles by S, and disappearing from older centrioles at telophase as the protein is recruited to the midbody. Our data show that TBCD overexpression results in microtubule release from the centrosome and G1 arrest, whereas its depletion produces mitotic aberrations and incomplete microtubule retraction at the midbody during cytokinesis. TBCD is recruited to the centriole replication site at the onset of the centrosome duplication cycle. A role in centriologenesis is further supported in differentiating ciliated cells, where TBCD is organized into "centriolar rosettes". These data suggest that TBCD participates in both canonical and de novo centriolar assembly pathways.
format	article
author	Mónica López Fanarraga Javier Bellido Cristina Jaén Juan Carlos Villegas Juan Carlos Zabala
author_facet	Mónica López Fanarraga Javier Bellido Cristina Jaén Juan Carlos Villegas Juan Carlos Zabala
author_sort	Mónica López Fanarraga
title	TBCD links centriologenesis, spindle microtubule dynamics, and midbody abscission in human cells.
title_short	TBCD links centriologenesis, spindle microtubule dynamics, and midbody abscission in human cells.
title_full	TBCD links centriologenesis, spindle microtubule dynamics, and midbody abscission in human cells.
title_fullStr	TBCD links centriologenesis, spindle microtubule dynamics, and midbody abscission in human cells.
title_full_unstemmed	TBCD links centriologenesis, spindle microtubule dynamics, and midbody abscission in human cells.
title_sort	tbcd links centriologenesis, spindle microtubule dynamics, and midbody abscission in human cells.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/f1fe0b63fff84631a5a5d7f6373dbfd0
work_keys_str_mv	AT monicalopezfanarraga tbcdlinkscentriologenesisspindlemicrotubuledynamicsandmidbodyabscissioninhumancells AT javierbellido tbcdlinkscentriologenesisspindlemicrotubuledynamicsandmidbodyabscissioninhumancells AT cristinajaen tbcdlinkscentriologenesisspindlemicrotubuledynamicsandmidbodyabscissioninhumancells AT juancarlosvillegas tbcdlinkscentriologenesisspindlemicrotubuledynamicsandmidbodyabscissioninhumancells AT juancarloszabala tbcdlinkscentriologenesisspindlemicrotubuledynamicsandmidbodyabscissioninhumancells
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TBCD links centriologenesis, spindle microtubule dynamics, and midbody abscission in human cells.

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