High throughput sequencing of RNA transcriptomes in Ruditapes philippinarum identifies genes involved in osmotic stress response

Abstract Ruditapes philippinarum, is an economically important marine bivalve species. The ability to cope with low salinity stress is quite important for the survival of aquatic species under natural conditions. In this study, the transcriptional response of the Manila clam to low salinity stress w...

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Autores principales: Hongtao Nie, Liwen Jiang, Peng Chen, Zhongming Huo, Feng Yang, Xiwu Yan
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Lenguaje:EN
Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/f20011b741e349fcb52d04f4fc692a16
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spelling oai:doaj.org-article:f20011b741e349fcb52d04f4fc692a162021-12-02T15:04:56ZHigh throughput sequencing of RNA transcriptomes in Ruditapes philippinarum identifies genes involved in osmotic stress response10.1038/s41598-017-05397-82045-2322https://doaj.org/article/f20011b741e349fcb52d04f4fc692a162017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05397-8https://doaj.org/toc/2045-2322Abstract Ruditapes philippinarum, is an economically important marine bivalve species. The ability to cope with low salinity stress is quite important for the survival of aquatic species under natural conditions. In this study, the transcriptional response of the Manila clam to low salinity stress was characterized using RNA sequencing. The transcriptomes of a low salinity-treatment group (FRp1, FRp2), which survived under low salinity stress, and control group (SRp1, SRp2), which was not subjected to low salinity stress, were sequenced with the Illumina HiSeq platform. A total of 196,578 unigenes were generated. GO and KEGG analyses revealed that signal transduction, immune response, cellular component organization or biogenesis, and energy production processes were the most highly enriched pathways among the genes that were differentially expressed under low salinity stress. All these pathways could be assigned to the following biological functions in the low salinity tolerant Manila clam: signal response to low salinity stress, antioxidant response, intracellular free amino acid transport and metabolism, energy production and conversion, cell signaling pathways, and regulation of ionic content and cell volume. In summary, this is the first study using high-throughput sequencing to identify gene targets that could explain osmotic regulation mechanisms under salinity stress in R. philippinarum.Hongtao NieLiwen JiangPeng ChenZhongming HuoFeng YangXiwu YanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Hongtao Nie
Liwen Jiang
Peng Chen
Zhongming Huo
Feng Yang
Xiwu Yan
High throughput sequencing of RNA transcriptomes in Ruditapes philippinarum identifies genes involved in osmotic stress response
description Abstract Ruditapes philippinarum, is an economically important marine bivalve species. The ability to cope with low salinity stress is quite important for the survival of aquatic species under natural conditions. In this study, the transcriptional response of the Manila clam to low salinity stress was characterized using RNA sequencing. The transcriptomes of a low salinity-treatment group (FRp1, FRp2), which survived under low salinity stress, and control group (SRp1, SRp2), which was not subjected to low salinity stress, were sequenced with the Illumina HiSeq platform. A total of 196,578 unigenes were generated. GO and KEGG analyses revealed that signal transduction, immune response, cellular component organization or biogenesis, and energy production processes were the most highly enriched pathways among the genes that were differentially expressed under low salinity stress. All these pathways could be assigned to the following biological functions in the low salinity tolerant Manila clam: signal response to low salinity stress, antioxidant response, intracellular free amino acid transport and metabolism, energy production and conversion, cell signaling pathways, and regulation of ionic content and cell volume. In summary, this is the first study using high-throughput sequencing to identify gene targets that could explain osmotic regulation mechanisms under salinity stress in R. philippinarum.
format article
author Hongtao Nie
Liwen Jiang
Peng Chen
Zhongming Huo
Feng Yang
Xiwu Yan
author_facet Hongtao Nie
Liwen Jiang
Peng Chen
Zhongming Huo
Feng Yang
Xiwu Yan
author_sort Hongtao Nie
title High throughput sequencing of RNA transcriptomes in Ruditapes philippinarum identifies genes involved in osmotic stress response
title_short High throughput sequencing of RNA transcriptomes in Ruditapes philippinarum identifies genes involved in osmotic stress response
title_full High throughput sequencing of RNA transcriptomes in Ruditapes philippinarum identifies genes involved in osmotic stress response
title_fullStr High throughput sequencing of RNA transcriptomes in Ruditapes philippinarum identifies genes involved in osmotic stress response
title_full_unstemmed High throughput sequencing of RNA transcriptomes in Ruditapes philippinarum identifies genes involved in osmotic stress response
title_sort high throughput sequencing of rna transcriptomes in ruditapes philippinarum identifies genes involved in osmotic stress response
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/f20011b741e349fcb52d04f4fc692a16
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