Enhancement of Th1/Th17 inflammation by TRIM21 in Behçet’s disease

Abstract The etiology of Behçet’s disease (BD), a chronic, multisystemic autoinflammatory and autoimmune disease, remains unknown; however, researchers have postulated that infectious agents, such as herpes simplex virus, are significant triggering factors of BD. Tripartite motif-containing (TRIM) p...

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Autores principales:	Yuri Ahn, Ji-Hye Hwang, Zhenlong Zheng, Dongsik Bang, Do-Young Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f20785cf04064183990f309bf9e197ed
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spelling	oai:doaj.org-article:f20785cf04064183990f309bf9e197ed2021-12-02T15:06:09ZEnhancement of Th1/Th17 inflammation by TRIM21 in Behçet’s disease10.1038/s41598-017-03251-52045-2322https://doaj.org/article/f20785cf04064183990f309bf9e197ed2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03251-5https://doaj.org/toc/2045-2322Abstract The etiology of Behçet’s disease (BD), a chronic, multisystemic autoinflammatory and autoimmune disease, remains unknown; however, researchers have postulated that infectious agents, such as herpes simplex virus, are significant triggering factors of BD. Tripartite motif-containing (TRIM) proteins exhibit antiviral properties, mediating antiviral defense mechanisms. The purpose of this study was to investigate TRIM21 protein expression in the monocytes of BD patients and to identify the role of TRIM21 in immune dysregulation in BD. In this study, the expression of TRIM21 and related molecules, including interferon regulatory factor 8 (IRF8), was analyzed in monocytes from BD patients. Functional analyses using small interfering RNA and co-culture with responder T cells were performed to examine the pathological role of TRIM21 in BD. Peripheral blood monocytes from BD patients showed increased TRIM21 expression and decreased IRF8 expression compared with that in monocytes from healthy controls. TRIM21 was found to decrease IRF8 expression. BD monocytes facilitated Th1 and Th17 differentiation of co-cultured T cells, and knock-down of TRIM21 expression by small interfering RNA inhibited this differentiation. In conclusion, TRIM21 played a pivotal role in regulating the secretion of proinflammatory cytokines in monocytes of BD patients.Yuri AhnJi-Hye HwangZhenlong ZhengDongsik BangDo-Young KimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuri Ahn Ji-Hye Hwang Zhenlong Zheng Dongsik Bang Do-Young Kim Enhancement of Th1/Th17 inflammation by TRIM21 in Behçet’s disease
description	Abstract The etiology of Behçet’s disease (BD), a chronic, multisystemic autoinflammatory and autoimmune disease, remains unknown; however, researchers have postulated that infectious agents, such as herpes simplex virus, are significant triggering factors of BD. Tripartite motif-containing (TRIM) proteins exhibit antiviral properties, mediating antiviral defense mechanisms. The purpose of this study was to investigate TRIM21 protein expression in the monocytes of BD patients and to identify the role of TRIM21 in immune dysregulation in BD. In this study, the expression of TRIM21 and related molecules, including interferon regulatory factor 8 (IRF8), was analyzed in monocytes from BD patients. Functional analyses using small interfering RNA and co-culture with responder T cells were performed to examine the pathological role of TRIM21 in BD. Peripheral blood monocytes from BD patients showed increased TRIM21 expression and decreased IRF8 expression compared with that in monocytes from healthy controls. TRIM21 was found to decrease IRF8 expression. BD monocytes facilitated Th1 and Th17 differentiation of co-cultured T cells, and knock-down of TRIM21 expression by small interfering RNA inhibited this differentiation. In conclusion, TRIM21 played a pivotal role in regulating the secretion of proinflammatory cytokines in monocytes of BD patients.
format	article
author	Yuri Ahn Ji-Hye Hwang Zhenlong Zheng Dongsik Bang Do-Young Kim
author_facet	Yuri Ahn Ji-Hye Hwang Zhenlong Zheng Dongsik Bang Do-Young Kim
author_sort	Yuri Ahn
title	Enhancement of Th1/Th17 inflammation by TRIM21 in Behçet’s disease
title_short	Enhancement of Th1/Th17 inflammation by TRIM21 in Behçet’s disease
title_full	Enhancement of Th1/Th17 inflammation by TRIM21 in Behçet’s disease
title_fullStr	Enhancement of Th1/Th17 inflammation by TRIM21 in Behçet’s disease
title_full_unstemmed	Enhancement of Th1/Th17 inflammation by TRIM21 in Behçet’s disease
title_sort	enhancement of th1/th17 inflammation by trim21 in behçet’s disease
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f20785cf04064183990f309bf9e197ed
work_keys_str_mv	AT yuriahn enhancementofth1th17inflammationbytrim21inbehcetsdisease AT jihyehwang enhancementofth1th17inflammationbytrim21inbehcetsdisease AT zhenlongzheng enhancementofth1th17inflammationbytrim21inbehcetsdisease AT dongsikbang enhancementofth1th17inflammationbytrim21inbehcetsdisease AT doyoungkim enhancementofth1th17inflammationbytrim21inbehcetsdisease
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Enhancement of Th1/Th17 inflammation by TRIM21 in Behçet’s disease

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