Photosystem II Repair Cycle in Faba Bean May Play a Role in Its Resistance to Botrytis fabae Infection

Chocolate spot, which is caused by the necrotrophic fungus Botrytis fabae, is a major foliar disease occurring worldwide and dramatically reducing crop yields in faba bean (Vicia faba). Although chemical control of this disease is an option, it has serious econo...

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Autores principales:	María Ángeles Castillejo, Ángel M. Villegas-Fernández, Tamara Hernández-Lao, Diego Rubiales
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Botrytis fabae</i> faba bean resistance proteomic analysis photosystem II repair cycle Agriculture S
Acceso en línea:	https://doaj.org/article/f2081763a0d04d1b9099fc93e8e43244
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spelling	oai:doaj.org-article:f2081763a0d04d1b9099fc93e8e432442021-11-25T16:08:34ZPhotosystem II Repair Cycle in Faba Bean May Play a Role in Its Resistance to <i>Botrytis fabae</i> Infection10.3390/agronomy111122472073-4395https://doaj.org/article/f2081763a0d04d1b9099fc93e8e432442021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4395/11/11/2247https://doaj.org/toc/2073-4395Chocolate spot, which is caused by the necrotrophic fungus <i>Botrytis fabae</i>, is a major foliar disease occurring worldwide and dramatically reducing crop yields in faba bean (<i>Vicia faba</i>). Although chemical control of this disease is an option, it has serious economic and environmental drawbacks that make resistant cultivars a more sensible choice. The molecular mechanisms behind the defense against <i>B. fabae</i> are poorly understood. In this work, we studied the leave proteome in two faba bean genotypes that respond differently to <i>B. fabae</i> in order to expand the available knowledge on such mechanisms. For this purpose, we used two-dimensional gel electrophoresis (2DE) in combination with Matrix-Assisted Laser Desorption/Ionization (MALDI-TOF/TOF). Univariate statistical analysis of the gels revealed 194 differential protein spots, 102 of which were identified by mass spectrometry. Most of the spots belonged to proteins in the energy and primary metabolism, degradation, redox or response to stress functional groups. The MS results were validated with assays of protease activity in gels. Overall, they suggest that the two genotypes may respond to <i>B. fabae</i> with a different PSII protein repair cycle mechanism in the chloroplast. The differences in resistance to <i>B. fabae</i> may be the result of a metabolic imbalance in the susceptible genotype and of a more efficient chloroplast detoxification system in the resistant genotype at the early stages of infection.María Ángeles CastillejoÁngel M. Villegas-FernándezTamara Hernández-LaoDiego RubialesMDPI AGarticle<i>Botrytis fabae</i>faba beanresistanceproteomic analysisphotosystem II repair cycleAgricultureSENAgronomy, Vol 11, Iss 2247, p 2247 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Botrytis fabae</i> faba bean resistance proteomic analysis photosystem II repair cycle Agriculture S
spellingShingle	<i>Botrytis fabae</i> faba bean resistance proteomic analysis photosystem II repair cycle Agriculture S María Ángeles Castillejo Ángel M. Villegas-Fernández Tamara Hernández-Lao Diego Rubiales Photosystem II Repair Cycle in Faba Bean May Play a Role in Its Resistance to <i>Botrytis fabae</i> Infection
description	Chocolate spot, which is caused by the necrotrophic fungus <i>Botrytis fabae</i>, is a major foliar disease occurring worldwide and dramatically reducing crop yields in faba bean (<i>Vicia faba</i>). Although chemical control of this disease is an option, it has serious economic and environmental drawbacks that make resistant cultivars a more sensible choice. The molecular mechanisms behind the defense against <i>B. fabae</i> are poorly understood. In this work, we studied the leave proteome in two faba bean genotypes that respond differently to <i>B. fabae</i> in order to expand the available knowledge on such mechanisms. For this purpose, we used two-dimensional gel electrophoresis (2DE) in combination with Matrix-Assisted Laser Desorption/Ionization (MALDI-TOF/TOF). Univariate statistical analysis of the gels revealed 194 differential protein spots, 102 of which were identified by mass spectrometry. Most of the spots belonged to proteins in the energy and primary metabolism, degradation, redox or response to stress functional groups. The MS results were validated with assays of protease activity in gels. Overall, they suggest that the two genotypes may respond to <i>B. fabae</i> with a different PSII protein repair cycle mechanism in the chloroplast. The differences in resistance to <i>B. fabae</i> may be the result of a metabolic imbalance in the susceptible genotype and of a more efficient chloroplast detoxification system in the resistant genotype at the early stages of infection.
format	article
author	María Ángeles Castillejo Ángel M. Villegas-Fernández Tamara Hernández-Lao Diego Rubiales
author_facet	María Ángeles Castillejo Ángel M. Villegas-Fernández Tamara Hernández-Lao Diego Rubiales
author_sort	María Ángeles Castillejo
title	Photosystem II Repair Cycle in Faba Bean May Play a Role in Its Resistance to <i>Botrytis fabae</i> Infection
title_short	Photosystem II Repair Cycle in Faba Bean May Play a Role in Its Resistance to <i>Botrytis fabae</i> Infection
title_full	Photosystem II Repair Cycle in Faba Bean May Play a Role in Its Resistance to <i>Botrytis fabae</i> Infection
title_fullStr	Photosystem II Repair Cycle in Faba Bean May Play a Role in Its Resistance to <i>Botrytis fabae</i> Infection
title_full_unstemmed	Photosystem II Repair Cycle in Faba Bean May Play a Role in Its Resistance to <i>Botrytis fabae</i> Infection
title_sort	photosystem ii repair cycle in faba bean may play a role in its resistance to <i>botrytis fabae</i> infection
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f2081763a0d04d1b9099fc93e8e43244
work_keys_str_mv	AT mariaangelescastillejo photosystemiirepaircycleinfababeanmayplayaroleinitsresistancetoibotrytisfabaeiinfection AT angelmvillegasfernandez photosystemiirepaircycleinfababeanmayplayaroleinitsresistancetoibotrytisfabaeiinfection AT tamarahernandezlao photosystemiirepaircycleinfababeanmayplayaroleinitsresistancetoibotrytisfabaeiinfection AT diegorubiales photosystemiirepaircycleinfababeanmayplayaroleinitsresistancetoibotrytisfabaeiinfection
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Photosystem II Repair Cycle in Faba Bean May Play a Role in Its Resistance to <i>Botrytis fabae</i> Infection

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