Hippocampal miRNA-144 Modulates Depressive-Like Behaviors in Rats by Targeting PTP1B

Yuhuan Li,1 Nina Wang,2 Jie Pan,2 Xinrui Wang,1 Yanling Zhao,3 Zongjun Guo4 1Department of Psychology, Qingdao Mental Health Center, Qingdao City, Shandong Province, 266000, People’s Republic of China; 2Department of Pharmacy, Qingdao Mental Health Center, Qingdao City, Shandong Province,...

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Autores principales:	Li Y, Wang N, Pan J, Wang X, Zhao Y, Guo Z
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	mir-144 cums hippocampus ptp1b trkb/bdnf Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/f208a89ae6b0426d989a9bb447d1f0c3
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spelling	oai:doaj.org-article:f208a89ae6b0426d989a9bb447d1f0c32021-12-02T12:14:52ZHippocampal miRNA-144 Modulates Depressive-Like Behaviors in Rats by Targeting PTP1B1178-2021https://doaj.org/article/f208a89ae6b0426d989a9bb447d1f0c32021-02-01T00:00:00Zhttps://www.dovepress.com/hippocampal-mirna-144-modulates-depressive-like-behaviors-in-rats-by-t-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Yuhuan Li,1 Nina Wang,2 Jie Pan,2 Xinrui Wang,1 Yanling Zhao,3 Zongjun Guo4 1Department of Psychology, Qingdao Mental Health Center, Qingdao City, Shandong Province, 266000, People’s Republic of China; 2Department of Pharmacy, Qingdao Mental Health Center, Qingdao City, Shandong Province, 266000, People’s Republic of China; 3Department of Methadone Clinic, Qingdao Mental Health Center, Qingdao City, Shandong Province, 266000, People’s Republic of China; 4Department of Geriatric Internal Medicine, The Affiliated Hospital of Qingdao University, Qingdao City, Shandong Province, 266000, People’s Republic of ChinaCorrespondence: Xinrui WangDepartment of Psychology, Qingdao Mental Health Center, No. 9 Dong ‘an Road, North District, Qingdao City, Shandong Province, 266000, People’s Republic of ChinaTel +86 532-8093158Email ffssmh256766237@126.comBackground: Depression is the common mental disorder in the world. However, the pathophysiology mechanism underlying depression remains elusive. It has been reported that aberrant expression of miR-144 is closely related to depression. This study was to investigate whether and how miR-144 involves in depressive-like behaviors in a chronic unpredictable mild stress (CUMS) animal model.Methods: A rat model of CUMS was established, and qRT-PCR was performed to detect the expression of miR-144 in the hippocampus of a depressed rat. The lentiviral vector carried miR-144 (LV-miR-144) was injected into the hippocampus of the CUMS rat to investigate the effects of miR-144 on the behaviors and PTP1B/TrkB/BDNF signal transduction in the hippocampus of the rat. The interaction between miR-144 and PTP1B was investigated by biological analyses and dual-luciferase reporter assay.Results: The results showed that CUMS rats had typical depressive behaviors, and the expression of miR-144 in the hippocampus of CUMS rats was significantly lower than that of the control group. In addition, PTP1B protein expression was significantly up-regulated, while the expression of pTrkB and BDNF protein was significantly down-regulated in the hippocampus of CUMS rats. Moreover, PTP1B was a direct target of miR-144, and miR-144 could activate the downstream TrkB/BDNF signaling pathway by inhibiting the expression of PTP1B in primary hippocampus neurons.Conclusion: MiR-144 played an anti-depressive role in hippocampus dysfunction by inhibiting PTP1B and activating the TrkB/BDNF signaling pathway in the hippocampus of CUMS rats.Keywords: miR-144, CUMS, hippocampus, PTP1B, TrkB/BDNFLi YWang NPan JWang XZhao YGuo ZDove Medical Pressarticlemir-144cumshippocampusptp1btrkb/bdnfNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 17, Pp 389-399 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mir-144 cums hippocampus ptp1b trkb/bdnf Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	mir-144 cums hippocampus ptp1b trkb/bdnf Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Li Y Wang N Pan J Wang X Zhao Y Guo Z Hippocampal miRNA-144 Modulates Depressive-Like Behaviors in Rats by Targeting PTP1B
description	Yuhuan Li,1 Nina Wang,2 Jie Pan,2 Xinrui Wang,1 Yanling Zhao,3 Zongjun Guo4 1Department of Psychology, Qingdao Mental Health Center, Qingdao City, Shandong Province, 266000, People’s Republic of China; 2Department of Pharmacy, Qingdao Mental Health Center, Qingdao City, Shandong Province, 266000, People’s Republic of China; 3Department of Methadone Clinic, Qingdao Mental Health Center, Qingdao City, Shandong Province, 266000, People’s Republic of China; 4Department of Geriatric Internal Medicine, The Affiliated Hospital of Qingdao University, Qingdao City, Shandong Province, 266000, People’s Republic of ChinaCorrespondence: Xinrui WangDepartment of Psychology, Qingdao Mental Health Center, No. 9 Dong ‘an Road, North District, Qingdao City, Shandong Province, 266000, People’s Republic of ChinaTel +86 532-8093158Email ffssmh256766237@126.comBackground: Depression is the common mental disorder in the world. However, the pathophysiology mechanism underlying depression remains elusive. It has been reported that aberrant expression of miR-144 is closely related to depression. This study was to investigate whether and how miR-144 involves in depressive-like behaviors in a chronic unpredictable mild stress (CUMS) animal model.Methods: A rat model of CUMS was established, and qRT-PCR was performed to detect the expression of miR-144 in the hippocampus of a depressed rat. The lentiviral vector carried miR-144 (LV-miR-144) was injected into the hippocampus of the CUMS rat to investigate the effects of miR-144 on the behaviors and PTP1B/TrkB/BDNF signal transduction in the hippocampus of the rat. The interaction between miR-144 and PTP1B was investigated by biological analyses and dual-luciferase reporter assay.Results: The results showed that CUMS rats had typical depressive behaviors, and the expression of miR-144 in the hippocampus of CUMS rats was significantly lower than that of the control group. In addition, PTP1B protein expression was significantly up-regulated, while the expression of pTrkB and BDNF protein was significantly down-regulated in the hippocampus of CUMS rats. Moreover, PTP1B was a direct target of miR-144, and miR-144 could activate the downstream TrkB/BDNF signaling pathway by inhibiting the expression of PTP1B in primary hippocampus neurons.Conclusion: MiR-144 played an anti-depressive role in hippocampus dysfunction by inhibiting PTP1B and activating the TrkB/BDNF signaling pathway in the hippocampus of CUMS rats.Keywords: miR-144, CUMS, hippocampus, PTP1B, TrkB/BDNF
format	article
author	Li Y Wang N Pan J Wang X Zhao Y Guo Z
author_facet	Li Y Wang N Pan J Wang X Zhao Y Guo Z
author_sort	Li Y
title	Hippocampal miRNA-144 Modulates Depressive-Like Behaviors in Rats by Targeting PTP1B
title_short	Hippocampal miRNA-144 Modulates Depressive-Like Behaviors in Rats by Targeting PTP1B
title_full	Hippocampal miRNA-144 Modulates Depressive-Like Behaviors in Rats by Targeting PTP1B
title_fullStr	Hippocampal miRNA-144 Modulates Depressive-Like Behaviors in Rats by Targeting PTP1B
title_full_unstemmed	Hippocampal miRNA-144 Modulates Depressive-Like Behaviors in Rats by Targeting PTP1B
title_sort	hippocampal mirna-144 modulates depressive-like behaviors in rats by targeting ptp1b
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/f208a89ae6b0426d989a9bb447d1f0c3
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Hippocampal miRNA-144 Modulates Depressive-Like Behaviors in Rats by Targeting PTP1B

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