Profile of subpopulation composition of regulatory T lymphocytes and intestinal microbiota in patients with irritable bowel syndrome

The following specificcharacteristics of the composition of intestinal microbiota in patients with irritable bowel syndrome (IBS) were identified using a metagenomic analysis (16 S rRNA): 1) an increase in the representation of Actinobacteria, including Bifidobacterium spp., Firmicutes, including re...

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Autores principales:	E. I. Ermolenko, I. V. Kudryavtsev, O. I. Solovyova, A. B. Karaseva, A. N. Tsapieva, Yu. D. Kondratenko, M. P. Kotyleva, M. K. Serebryakova, I. A. Shumikhina, A. N. Suvorov
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2020
Materias:	intestinal microbiocenosis metagenomic analysis flow cytometry of a subpopulation of t lymphocytes Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/f21cd7d917eb4f0db65244cd22e3c152
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spelling	oai:doaj.org-article:f21cd7d917eb4f0db65244cd22e3c1522021-11-18T08:03:49ZProfile of subpopulation composition of regulatory T lymphocytes and intestinal microbiota in patients with irritable bowel syndrome1563-06252313-741X10.15789/1563-0625-POS-1905https://doaj.org/article/f21cd7d917eb4f0db65244cd22e3c1522020-04-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1905https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XThe following specificcharacteristics of the composition of intestinal microbiota in patients with irritable bowel syndrome (IBS) were identified using a metagenomic analysis (16 S rRNA): 1) an increase in the representation of Actinobacteria, including Bifidobacterium spp., Firmicutes, including representatives of Streptococcaceae (Streptococcus), Lachnosperaceae (Dorea), Veillonellaceae (Dialister), Proteobacteria (Enterobacteriaceae and Desulfovibrionaceae families); 2) a decrease in the population of Bacteroidetes, including representatives of the families Prevotellacea (Prevotella spp.), Bacteroidaceae (Bacteroides spp.). Firmicutes belonging to the families Clostridiaceae and Ruminococcaceae (Fecalibacterium spp.).Flow cytometry in the study of the subpopulation composition of T regulatory (Treg) lymphocytes in patients with IBS revealed an increase in the number of CD45R0+CD62L+ central memory cells (CM), which can regulate the processes of maturation and differentiation of lymphocytes in lymphoid tissue. A decrease in the expression of exonucleases CD39 and CD73 was detected, which can have a significant effect on their activity. A reduction in effector memory cells (EM) Treg was observed.Changes in the expression level of exonucleases CD39 and CD73 were inversely correlated with the content of Proteobacteria and the representation of the genera Bifidobacterium spp. and Faecalibacterium spp. The content of СЫ Treg was directly correlated with the content of Dorea spp.The results may be indicative of impairment in the processes of Treg differentiation, which are closely related to changes in key components of intestinal microbiocenosis in IBS.E. I. ErmolenkoI. V. KudryavtsevO. I. SolovyovaA. B. KarasevaA. N. TsapievaYu. D. KondratenkoM. P. KotylevaM. K. SerebryakovaI. A. ShumikhinaA. N. SuvorovSPb RAACIarticleintestinal microbiocenosismetagenomic analysisflow cytometry of a subpopulation of t lymphocytesImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 22, Iss 2, Pp 335-346 (2020)
institution	DOAJ
collection	DOAJ
language	RU
topic	intestinal microbiocenosis metagenomic analysis flow cytometry of a subpopulation of t lymphocytes Immunologic diseases. Allergy RC581-607
spellingShingle	intestinal microbiocenosis metagenomic analysis flow cytometry of a subpopulation of t lymphocytes Immunologic diseases. Allergy RC581-607 E. I. Ermolenko I. V. Kudryavtsev O. I. Solovyova A. B. Karaseva A. N. Tsapieva Yu. D. Kondratenko M. P. Kotyleva M. K. Serebryakova I. A. Shumikhina A. N. Suvorov Profile of subpopulation composition of regulatory T lymphocytes and intestinal microbiota in patients with irritable bowel syndrome
description	The following specificcharacteristics of the composition of intestinal microbiota in patients with irritable bowel syndrome (IBS) were identified using a metagenomic analysis (16 S rRNA): 1) an increase in the representation of Actinobacteria, including Bifidobacterium spp., Firmicutes, including representatives of Streptococcaceae (Streptococcus), Lachnosperaceae (Dorea), Veillonellaceae (Dialister), Proteobacteria (Enterobacteriaceae and Desulfovibrionaceae families); 2) a decrease in the population of Bacteroidetes, including representatives of the families Prevotellacea (Prevotella spp.), Bacteroidaceae (Bacteroides spp.). Firmicutes belonging to the families Clostridiaceae and Ruminococcaceae (Fecalibacterium spp.).Flow cytometry in the study of the subpopulation composition of T regulatory (Treg) lymphocytes in patients with IBS revealed an increase in the number of CD45R0+CD62L+ central memory cells (CM), which can regulate the processes of maturation and differentiation of lymphocytes in lymphoid tissue. A decrease in the expression of exonucleases CD39 and CD73 was detected, which can have a significant effect on their activity. A reduction in effector memory cells (EM) Treg was observed.Changes in the expression level of exonucleases CD39 and CD73 were inversely correlated with the content of Proteobacteria and the representation of the genera Bifidobacterium spp. and Faecalibacterium spp. The content of СЫ Treg was directly correlated with the content of Dorea spp.The results may be indicative of impairment in the processes of Treg differentiation, which are closely related to changes in key components of intestinal microbiocenosis in IBS.
format	article
author	E. I. Ermolenko I. V. Kudryavtsev O. I. Solovyova A. B. Karaseva A. N. Tsapieva Yu. D. Kondratenko M. P. Kotyleva M. K. Serebryakova I. A. Shumikhina A. N. Suvorov
author_facet	E. I. Ermolenko I. V. Kudryavtsev O. I. Solovyova A. B. Karaseva A. N. Tsapieva Yu. D. Kondratenko M. P. Kotyleva M. K. Serebryakova I. A. Shumikhina A. N. Suvorov
author_sort	E. I. Ermolenko
title	Profile of subpopulation composition of regulatory T lymphocytes and intestinal microbiota in patients with irritable bowel syndrome
title_short	Profile of subpopulation composition of regulatory T lymphocytes and intestinal microbiota in patients with irritable bowel syndrome
title_full	Profile of subpopulation composition of regulatory T lymphocytes and intestinal microbiota in patients with irritable bowel syndrome
title_fullStr	Profile of subpopulation composition of regulatory T lymphocytes and intestinal microbiota in patients with irritable bowel syndrome
title_full_unstemmed	Profile of subpopulation composition of regulatory T lymphocytes and intestinal microbiota in patients with irritable bowel syndrome
title_sort	profile of subpopulation composition of regulatory t lymphocytes and intestinal microbiota in patients with irritable bowel syndrome
publisher	SPb RAACI
publishDate	2020
url	https://doaj.org/article/f21cd7d917eb4f0db65244cd22e3c152
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Profile of subpopulation composition of regulatory T lymphocytes and intestinal microbiota in patients with irritable bowel syndrome

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