The RNA N6-Methyladenosine Demethylase FTO Promotes Head and Neck Squamous Cell Carcinoma Proliferation and Migration by Increasing CTNNB1

Yu Zhang,1 Lixiao Chen,1 Xiaoliang Wu,2 Zhenfeng Sun,1 Fei Wang,1 Baoxin Wang,1 Pin Dong1 1Department of Otolaryngology, Head and Neck Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China; 2Tailai Bioscience, Shenzhen, People’s Re...

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Autores principales:	Zhang Y, Chen L, Wu X, Sun Z, Wang F, Wang B, Dong P
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	fto m6a hnscc ctnnb1 Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/f2201d57b3364cfa8f2d45ab977c6997
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spelling	oai:doaj.org-article:f2201d57b3364cfa8f2d45ab977c69972021-11-23T18:43:01ZThe RNA N6-Methyladenosine Demethylase FTO Promotes Head and Neck Squamous Cell Carcinoma Proliferation and Migration by Increasing CTNNB11178-7074https://doaj.org/article/f2201d57b3364cfa8f2d45ab977c69972021-11-01T00:00:00Zhttps://www.dovepress.com/the-rna-n6-methyladenosine-demethylase-fto-promotes-head-and-neck-squa-peer-reviewed-fulltext-article-IJGMhttps://doaj.org/toc/1178-7074Yu Zhang,1 Lixiao Chen,1 Xiaoliang Wu,2 Zhenfeng Sun,1 Fei Wang,1 Baoxin Wang,1 Pin Dong1 1Department of Otolaryngology, Head and Neck Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China; 2Tailai Bioscience, Shenzhen, People’s Republic of ChinaCorrespondence: Pin DongDepartment of Otolaryngology, Head and Neck Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, No. 85 Wujin Road, Hongkou District, Shanghai, 200080, People’s Republic of ChinaEmail dongpin64@aliyun.comObjective: In this study, we aimed to investigate the role of RNA N6-methyladenosine demethylase fat mass and obesity-associated protein (FTO) in head and neck squamous cell carcinoma (HNSCC).Methods: Clinical data downloaded from The Cancer Genome Atlas (TCGA) database were used to analyze the relationship between mRNA levels of FTO, METTL3, METTL14, and ALKBH5, and the overall survival in cancer and para-cancer datasets. FTO expression in tumor and normal tissues was compared using immunohistochemistry, and its relationship with overall survival was analyzed based on the Kaplan–Meier method. The FaDu cell line with high FTO levels was chosen from five HNSCC cell lines for further experiments. FTO was verified as an oncogene in HNSCC by in vitro loss-of-function and overexpression studies, cell proliferation assay, wound healing assay, and identification of expression changes of epithelial–mesenchymal transition (EMT)-related markers. Catenin beta 1 (CTNNB1) was confirmed as a downstream target gene of FTO with additional methods like the GEPIA online tool, qRT-PCR, Western blotting, and dot blot assay.Results: We found that FTO expression was significantly upregulated in HNSCC datasets and tissues. Increased FTO expression indicated a trend towards poor prognosis and was found to promote disease proliferation and migration. Mechanistically, cell proliferation assay, wound healing assay, and identification of expression changes of EMT-related markers demonstrated that FTO could act as an oncogene in HNSCC. FTO expression was significantly correlated with CTNNB1 expression. Moreover, it exerted a tumorigenic effect by increasing CTNNB1 expression in an m6A-dependent manner.Conclusion: FTO promotes head and neck squamous cell carcinoma proliferation and migration by increasing CTNNB1 in an m6A-dependent manner.Keywords: FTO, m6A, HNSCC, CTNNB1Zhang YChen LWu XSun ZWang FWang BDong PDove Medical Pressarticleftom6ahnsccctnnb1Medicine (General)R5-920ENInternational Journal of General Medicine, Vol Volume 14, Pp 8785-8795 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	fto m6a hnscc ctnnb1 Medicine (General) R5-920
spellingShingle	fto m6a hnscc ctnnb1 Medicine (General) R5-920 Zhang Y Chen L Wu X Sun Z Wang F Wang B Dong P The RNA N6-Methyladenosine Demethylase FTO Promotes Head and Neck Squamous Cell Carcinoma Proliferation and Migration by Increasing CTNNB1
description	Yu Zhang,1 Lixiao Chen,1 Xiaoliang Wu,2 Zhenfeng Sun,1 Fei Wang,1 Baoxin Wang,1 Pin Dong1 1Department of Otolaryngology, Head and Neck Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China; 2Tailai Bioscience, Shenzhen, People’s Republic of ChinaCorrespondence: Pin DongDepartment of Otolaryngology, Head and Neck Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, No. 85 Wujin Road, Hongkou District, Shanghai, 200080, People’s Republic of ChinaEmail dongpin64@aliyun.comObjective: In this study, we aimed to investigate the role of RNA N6-methyladenosine demethylase fat mass and obesity-associated protein (FTO) in head and neck squamous cell carcinoma (HNSCC).Methods: Clinical data downloaded from The Cancer Genome Atlas (TCGA) database were used to analyze the relationship between mRNA levels of FTO, METTL3, METTL14, and ALKBH5, and the overall survival in cancer and para-cancer datasets. FTO expression in tumor and normal tissues was compared using immunohistochemistry, and its relationship with overall survival was analyzed based on the Kaplan–Meier method. The FaDu cell line with high FTO levels was chosen from five HNSCC cell lines for further experiments. FTO was verified as an oncogene in HNSCC by in vitro loss-of-function and overexpression studies, cell proliferation assay, wound healing assay, and identification of expression changes of epithelial–mesenchymal transition (EMT)-related markers. Catenin beta 1 (CTNNB1) was confirmed as a downstream target gene of FTO with additional methods like the GEPIA online tool, qRT-PCR, Western blotting, and dot blot assay.Results: We found that FTO expression was significantly upregulated in HNSCC datasets and tissues. Increased FTO expression indicated a trend towards poor prognosis and was found to promote disease proliferation and migration. Mechanistically, cell proliferation assay, wound healing assay, and identification of expression changes of EMT-related markers demonstrated that FTO could act as an oncogene in HNSCC. FTO expression was significantly correlated with CTNNB1 expression. Moreover, it exerted a tumorigenic effect by increasing CTNNB1 expression in an m6A-dependent manner.Conclusion: FTO promotes head and neck squamous cell carcinoma proliferation and migration by increasing CTNNB1 in an m6A-dependent manner.Keywords: FTO, m6A, HNSCC, CTNNB1
format	article
author	Zhang Y Chen L Wu X Sun Z Wang F Wang B Dong P
author_facet	Zhang Y Chen L Wu X Sun Z Wang F Wang B Dong P
author_sort	Zhang Y
title	The RNA N6-Methyladenosine Demethylase FTO Promotes Head and Neck Squamous Cell Carcinoma Proliferation and Migration by Increasing CTNNB1
title_short	The RNA N6-Methyladenosine Demethylase FTO Promotes Head and Neck Squamous Cell Carcinoma Proliferation and Migration by Increasing CTNNB1
title_full	The RNA N6-Methyladenosine Demethylase FTO Promotes Head and Neck Squamous Cell Carcinoma Proliferation and Migration by Increasing CTNNB1
title_fullStr	The RNA N6-Methyladenosine Demethylase FTO Promotes Head and Neck Squamous Cell Carcinoma Proliferation and Migration by Increasing CTNNB1
title_full_unstemmed	The RNA N6-Methyladenosine Demethylase FTO Promotes Head and Neck Squamous Cell Carcinoma Proliferation and Migration by Increasing CTNNB1
title_sort	rna n6-methyladenosine demethylase fto promotes head and neck squamous cell carcinoma proliferation and migration by increasing ctnnb1
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/f2201d57b3364cfa8f2d45ab977c6997
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The RNA N6-Methyladenosine Demethylase FTO Promotes Head and Neck Squamous Cell Carcinoma Proliferation and Migration by Increasing CTNNB1

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