Glycyrrhizic acid nanoparticles inhibit LPS-induced inflammatory mediators in 264.7 mouse macrophages compared with unprocessed glycyrrhizic acid

Wei Wang,1–3 Meng Luo,1–3 Yujie Fu,1–3 Song Wang,1–3 Thomas Efferth,4 Yuangang Zu1–3 1Key Laboratory of Forest Plant Ecology, 2Engineering Research Center of Forest Bio-Preparation, 3State Engineering Laboratory of Bio-Resource Eco-Utilization, North...

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Autores principales:	Wang W, Luo M, Fu Y, Wang S, Efferth T, Zu Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/f220db438bc34fd3b785cea3e716a2a4
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spelling	oai:doaj.org-article:f220db438bc34fd3b785cea3e716a2a42021-12-02T02:53:35ZGlycyrrhizic acid nanoparticles inhibit LPS-induced inflammatory mediators in 264.7 mouse macrophages compared with unprocessed glycyrrhizic acid1176-91141178-2013https://doaj.org/article/f220db438bc34fd3b785cea3e716a2a42013-04-01T00:00:00Zhttp://www.dovepress.com/glycyrrhizic-acid-nanoparticles-inhibit-lps-induced-inflammatory-media-a12739https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Wei Wang,1–3 Meng Luo,1–3 Yujie Fu,1–3 Song Wang,1–3 Thomas Efferth,4 Yuangang Zu1–3 1Key Laboratory of Forest Plant Ecology, 2Engineering Research Center of Forest Bio-Preparation, 3State Engineering Laboratory of Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin, China; 4Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, University of Mainz, Mainz, Germany Abstract: Glycyrrhizic acid (GA), the main component of radix glycyrrhizae, has a variety of pharmacological activities. In the present study, suspensions of GA nanoparticles with the average particle size about 200nm were prepared by a supercritical antisolvent (SAS) process. Comparative studies were undertaken using lipopolysaccardide(LPS)-stimulated mouse macrophages RAW 264.7 as in vitro inflammatory model. Several important inflammation mediators such as NO, PGE2, TNF-α and IL-6 were examined. These markers were highly stimulated by LPS and were inhibited both by nano-GA and unprocessed GA in a dose-dependent manner, especially PGE2 and TNF-α. However nano-GA and unprocessed GA inhibited NO only at a high concentration. In general, we found that GA nanoparticle suspensions exhibited much better anti-inflammatory activities compared to unprocessed GA. Keywords: glycyrrhizic acid, nanoparticle, mouse macrophages RAW 264.7, inflammatory cytokinesWang WLuo MFu YWang SEfferth TZu YDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 1377-1383 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Wang W Luo M Fu Y Wang S Efferth T Zu Y Glycyrrhizic acid nanoparticles inhibit LPS-induced inflammatory mediators in 264.7 mouse macrophages compared with unprocessed glycyrrhizic acid
description	Wei Wang,1–3 Meng Luo,1–3 Yujie Fu,1–3 Song Wang,1–3 Thomas Efferth,4 Yuangang Zu1–3 1Key Laboratory of Forest Plant Ecology, 2Engineering Research Center of Forest Bio-Preparation, 3State Engineering Laboratory of Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin, China; 4Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, University of Mainz, Mainz, Germany Abstract: Glycyrrhizic acid (GA), the main component of radix glycyrrhizae, has a variety of pharmacological activities. In the present study, suspensions of GA nanoparticles with the average particle size about 200nm were prepared by a supercritical antisolvent (SAS) process. Comparative studies were undertaken using lipopolysaccardide(LPS)-stimulated mouse macrophages RAW 264.7 as in vitro inflammatory model. Several important inflammation mediators such as NO, PGE2, TNF-α and IL-6 were examined. These markers were highly stimulated by LPS and were inhibited both by nano-GA and unprocessed GA in a dose-dependent manner, especially PGE2 and TNF-α. However nano-GA and unprocessed GA inhibited NO only at a high concentration. In general, we found that GA nanoparticle suspensions exhibited much better anti-inflammatory activities compared to unprocessed GA. Keywords: glycyrrhizic acid, nanoparticle, mouse macrophages RAW 264.7, inflammatory cytokines
format	article
author	Wang W Luo M Fu Y Wang S Efferth T Zu Y
author_facet	Wang W Luo M Fu Y Wang S Efferth T Zu Y
author_sort	Wang W
title	Glycyrrhizic acid nanoparticles inhibit LPS-induced inflammatory mediators in 264.7 mouse macrophages compared with unprocessed glycyrrhizic acid
title_short	Glycyrrhizic acid nanoparticles inhibit LPS-induced inflammatory mediators in 264.7 mouse macrophages compared with unprocessed glycyrrhizic acid
title_full	Glycyrrhizic acid nanoparticles inhibit LPS-induced inflammatory mediators in 264.7 mouse macrophages compared with unprocessed glycyrrhizic acid
title_fullStr	Glycyrrhizic acid nanoparticles inhibit LPS-induced inflammatory mediators in 264.7 mouse macrophages compared with unprocessed glycyrrhizic acid
title_full_unstemmed	Glycyrrhizic acid nanoparticles inhibit LPS-induced inflammatory mediators in 264.7 mouse macrophages compared with unprocessed glycyrrhizic acid
title_sort	glycyrrhizic acid nanoparticles inhibit lps-induced inflammatory mediators in 264.7 mouse macrophages compared with unprocessed glycyrrhizic acid
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/f220db438bc34fd3b785cea3e716a2a4
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Glycyrrhizic acid nanoparticles inhibit LPS-induced inflammatory mediators in 264.7 mouse macrophages compared with unprocessed glycyrrhizic acid

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