Baculovirus infection induces disruption of the nuclear lamina

Abstract Baculovirus nucleocapsids egress from the nucleus primarily via budding at the nuclear membrane. The nuclear lamina underlying the nuclear membrane represents a substantial barrier to nuclear egress. Whether the nuclear lamina undergoes disruption during baculovirus infection remains unknow...

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Autores principales:	Xiaomei Zhang, Kaiyan Xu, Denghui Wei, Wenbi Wu, Kai Yang, Meijin Yuan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f22f5c95103f4f69848a12dfb4b693f5
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spelling	oai:doaj.org-article:f22f5c95103f4f69848a12dfb4b693f52021-12-02T16:08:10ZBaculovirus infection induces disruption of the nuclear lamina10.1038/s41598-017-08437-52045-2322https://doaj.org/article/f22f5c95103f4f69848a12dfb4b693f52017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08437-5https://doaj.org/toc/2045-2322Abstract Baculovirus nucleocapsids egress from the nucleus primarily via budding at the nuclear membrane. The nuclear lamina underlying the nuclear membrane represents a substantial barrier to nuclear egress. Whether the nuclear lamina undergoes disruption during baculovirus infection remains unknown. In this report, we generated a clonal cell line, Sf9-L, that stably expresses GFP-tagged Drosophila lamin B. GFP autofluorescence colocalized with immunofluorescent anti-lamin B at the nuclear rim of Sf9-L cells, indicating GFP-lamin B was incorporated into the nuclear lamina. Meanwhile, virus was able to replicate normally in Sf9-L cells. Next, we investigated alterations to the nuclear lamina during baculovirus infection in Sf9-L cells. A portion of GFP-lamin B localized diffusely at the nuclear rim, and some GFP-lamin B was redistributed within the nucleus during the late phase of infection, suggesting the nuclear lamina was partially disrupted. Immunoelectron microscopy revealed associations between GFP-lamin B and the edges of the electron-dense stromal mattes of the virogenic stroma, intranuclear microvesicles, and ODV envelopes and nucleocapsids within the nucleus, indicating the release of some GFP-lamin B from the nuclear lamina. Additionally, GFP-lamin B phosphorylation increased upon infection. Based on these data, baculovirus infection induced lamin B phosphorylation and disruption of the nuclear lamina.Xiaomei ZhangKaiyan XuDenghui WeiWenbi WuKai YangMeijin YuanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaomei Zhang Kaiyan Xu Denghui Wei Wenbi Wu Kai Yang Meijin Yuan Baculovirus infection induces disruption of the nuclear lamina
description	Abstract Baculovirus nucleocapsids egress from the nucleus primarily via budding at the nuclear membrane. The nuclear lamina underlying the nuclear membrane represents a substantial barrier to nuclear egress. Whether the nuclear lamina undergoes disruption during baculovirus infection remains unknown. In this report, we generated a clonal cell line, Sf9-L, that stably expresses GFP-tagged Drosophila lamin B. GFP autofluorescence colocalized with immunofluorescent anti-lamin B at the nuclear rim of Sf9-L cells, indicating GFP-lamin B was incorporated into the nuclear lamina. Meanwhile, virus was able to replicate normally in Sf9-L cells. Next, we investigated alterations to the nuclear lamina during baculovirus infection in Sf9-L cells. A portion of GFP-lamin B localized diffusely at the nuclear rim, and some GFP-lamin B was redistributed within the nucleus during the late phase of infection, suggesting the nuclear lamina was partially disrupted. Immunoelectron microscopy revealed associations between GFP-lamin B and the edges of the electron-dense stromal mattes of the virogenic stroma, intranuclear microvesicles, and ODV envelopes and nucleocapsids within the nucleus, indicating the release of some GFP-lamin B from the nuclear lamina. Additionally, GFP-lamin B phosphorylation increased upon infection. Based on these data, baculovirus infection induced lamin B phosphorylation and disruption of the nuclear lamina.
format	article
author	Xiaomei Zhang Kaiyan Xu Denghui Wei Wenbi Wu Kai Yang Meijin Yuan
author_facet	Xiaomei Zhang Kaiyan Xu Denghui Wei Wenbi Wu Kai Yang Meijin Yuan
author_sort	Xiaomei Zhang
title	Baculovirus infection induces disruption of the nuclear lamina
title_short	Baculovirus infection induces disruption of the nuclear lamina
title_full	Baculovirus infection induces disruption of the nuclear lamina
title_fullStr	Baculovirus infection induces disruption of the nuclear lamina
title_full_unstemmed	Baculovirus infection induces disruption of the nuclear lamina
title_sort	baculovirus infection induces disruption of the nuclear lamina
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f22f5c95103f4f69848a12dfb4b693f5
work_keys_str_mv	AT xiaomeizhang baculovirusinfectioninducesdisruptionofthenuclearlamina AT kaiyanxu baculovirusinfectioninducesdisruptionofthenuclearlamina AT denghuiwei baculovirusinfectioninducesdisruptionofthenuclearlamina AT wenbiwu baculovirusinfectioninducesdisruptionofthenuclearlamina AT kaiyang baculovirusinfectioninducesdisruptionofthenuclearlamina AT meijinyuan baculovirusinfectioninducesdisruptionofthenuclearlamina
_version_	1718384630687596544

Baculovirus infection induces disruption of the nuclear lamina

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