Proteomic Studies on the Mechanism of Myostatin Regulating Cattle Skeletal Muscle Development
Myostatin (MSTN) is an important negative regulator of muscle growth and development. In this study, we performed comparatively the proteomics analyses of gluteus tissues from MSTN+/− Mongolian cattle (MG.MSTN+/−) and wild type Mongolian cattle (MG.WT) using a shotgun-based tandem mass tag (TMT) 6-p...
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2021
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oai:doaj.org-article:f232d15e3b6e471c99cd820ee956b95e2021-11-16T07:35:30ZProteomic Studies on the Mechanism of Myostatin Regulating Cattle Skeletal Muscle Development1664-802110.3389/fgene.2021.752129https://doaj.org/article/f232d15e3b6e471c99cd820ee956b95e2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fgene.2021.752129/fullhttps://doaj.org/toc/1664-8021Myostatin (MSTN) is an important negative regulator of muscle growth and development. In this study, we performed comparatively the proteomics analyses of gluteus tissues from MSTN+/− Mongolian cattle (MG.MSTN+/−) and wild type Mongolian cattle (MG.WT) using a shotgun-based tandem mass tag (TMT) 6-plex labeling method to investigate the regulation mechanism of MSTN on the growth and development of bovine skeletal muscle. A total of 1,950 proteins were identified in MG.MSTN+/− and MG.WT. Compared with MG.WT cattle, a total of 320 differentially expressed proteins were identified in MG.MSTN cattle, including 245 up-regulated differentially expressed proteins and 75 down-regulated differentially expressed proteins. Bioinformatics analysis showed that knockdown of the MSTN gene increased the expression of extracellular matrix and ribosome-related proteins, induced activation of focal adhesion, PI3K-AKT, and Ribosomal pathways. The results of proteomic analysis were verified by muscle tissue Western blot test and in vitro MSTN gene knockdown test, and it was found that knockdown MSTN gene expression could promote the proliferation and myogenic differentiation of bovine skeletal muscle satellite cells (BSMSCs). At the same time, Co-Immunoprecipitation (CO-IP) assay showed that MSTN gene interacted with extracellular matrix related protein type I collagen α 1 (COL1A1), and knocking down the expression of COL1A1 could inhibit the activity of adhesion, PI3K-AKT and ribosome pathway, thus inhibit BSMSCs proliferation. These results suggest that the MSTN gene regulates focal adhesion, PI3K-AKT, and Ribosomal pathway through the COL1A1 gene. In general, this study provides new insights into the regulatory mechanism of MSTN involved in muscle growth and development.Hui ShengYiwen GuoLinlin ZhangJunxing ZhangManning MiaoHaoyun TanDebao HuXin LiXiangbin DingGuangpeng LiHong GuoFrontiers Media S.A.articlemyostatinproteomicsextracellular matrixribosomefocal adhesionGeneticsQH426-470ENFrontiers in Genetics, Vol 12 (2021) |
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| topic |
myostatin proteomics extracellular matrix ribosome focal adhesion Genetics QH426-470 |
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myostatin proteomics extracellular matrix ribosome focal adhesion Genetics QH426-470 Hui Sheng Yiwen Guo Linlin Zhang Junxing Zhang Manning Miao Haoyun Tan Debao Hu Xin Li Xiangbin Ding Guangpeng Li Hong Guo Proteomic Studies on the Mechanism of Myostatin Regulating Cattle Skeletal Muscle Development |
| description |
Myostatin (MSTN) is an important negative regulator of muscle growth and development. In this study, we performed comparatively the proteomics analyses of gluteus tissues from MSTN+/− Mongolian cattle (MG.MSTN+/−) and wild type Mongolian cattle (MG.WT) using a shotgun-based tandem mass tag (TMT) 6-plex labeling method to investigate the regulation mechanism of MSTN on the growth and development of bovine skeletal muscle. A total of 1,950 proteins were identified in MG.MSTN+/− and MG.WT. Compared with MG.WT cattle, a total of 320 differentially expressed proteins were identified in MG.MSTN cattle, including 245 up-regulated differentially expressed proteins and 75 down-regulated differentially expressed proteins. Bioinformatics analysis showed that knockdown of the MSTN gene increased the expression of extracellular matrix and ribosome-related proteins, induced activation of focal adhesion, PI3K-AKT, and Ribosomal pathways. The results of proteomic analysis were verified by muscle tissue Western blot test and in vitro MSTN gene knockdown test, and it was found that knockdown MSTN gene expression could promote the proliferation and myogenic differentiation of bovine skeletal muscle satellite cells (BSMSCs). At the same time, Co-Immunoprecipitation (CO-IP) assay showed that MSTN gene interacted with extracellular matrix related protein type I collagen α 1 (COL1A1), and knocking down the expression of COL1A1 could inhibit the activity of adhesion, PI3K-AKT and ribosome pathway, thus inhibit BSMSCs proliferation. These results suggest that the MSTN gene regulates focal adhesion, PI3K-AKT, and Ribosomal pathway through the COL1A1 gene. In general, this study provides new insights into the regulatory mechanism of MSTN involved in muscle growth and development. |
| format |
article |
| author |
Hui Sheng Yiwen Guo Linlin Zhang Junxing Zhang Manning Miao Haoyun Tan Debao Hu Xin Li Xiangbin Ding Guangpeng Li Hong Guo |
| author_facet |
Hui Sheng Yiwen Guo Linlin Zhang Junxing Zhang Manning Miao Haoyun Tan Debao Hu Xin Li Xiangbin Ding Guangpeng Li Hong Guo |
| author_sort |
Hui Sheng |
| title |
Proteomic Studies on the Mechanism of Myostatin Regulating Cattle Skeletal Muscle Development |
| title_short |
Proteomic Studies on the Mechanism of Myostatin Regulating Cattle Skeletal Muscle Development |
| title_full |
Proteomic Studies on the Mechanism of Myostatin Regulating Cattle Skeletal Muscle Development |
| title_fullStr |
Proteomic Studies on the Mechanism of Myostatin Regulating Cattle Skeletal Muscle Development |
| title_full_unstemmed |
Proteomic Studies on the Mechanism of Myostatin Regulating Cattle Skeletal Muscle Development |
| title_sort |
proteomic studies on the mechanism of myostatin regulating cattle skeletal muscle development |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/f232d15e3b6e471c99cd820ee956b95e |
| work_keys_str_mv |
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