The cytokine secretion profile of mesenchymal stromal cells is determined by surface structure of the microenvironment

Abstract Mesenchymal stromal cells (MSC) secrete factors that contribute to organ homeostasis and repair in a tissue specific manner. For instance, kidney perivascular mesenchymal stromal cells (kPSCs) can facilitate renal epithelial repair through secretion of hepatocyte growth factor (HGF) while t...

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Autores principales:	Daniëlle G. Leuning, Nick R. M. Beijer, Nadia A. du Fossé, Steven Vermeulen, Ellen Lievers, Cees van Kooten, Ton J. Rabelink, Jan de Boer
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f23b075466864c9d9975c16924bc8abe
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spelling	oai:doaj.org-article:f23b075466864c9d9975c16924bc8abe2021-12-02T15:08:56ZThe cytokine secretion profile of mesenchymal stromal cells is determined by surface structure of the microenvironment10.1038/s41598-018-25700-52045-2322https://doaj.org/article/f23b075466864c9d9975c16924bc8abe2018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25700-5https://doaj.org/toc/2045-2322Abstract Mesenchymal stromal cells (MSC) secrete factors that contribute to organ homeostasis and repair in a tissue specific manner. For instance, kidney perivascular mesenchymal stromal cells (kPSCs) can facilitate renal epithelial repair through secretion of hepatocyte growth factor (HGF) while the secretome of bone marrow MSCs gives rise to immunosuppression. Stromal cells function in a complex 3-dimensional (3D) connective tissue architecture that induces conformational adaptation. Here we tested the hypothesis that surface topography and associated cell adaptations dictate stromal cell function through tuning of the cytokines released. To this end, we cultured human bone marrow and kidney perivascular stromal cells in the TopoWell plate, a custom-fabricated multi-well plate containing 76 unique bioactive surface topographies. Using fluorescent imaging, we observed profound changes in cell shape, accompanied by major quantitative changes in the secretory capacity of the MSCs. The cytokine secretion profile was closely related to cell morphology and was stromal cell type specific. Our data demonstrate that stromal cell function is determined by microenvironment structure and can be manipulated in an engineered setting. Our data also have implications for the clinical manufacturing of mesenchymal stromal cell therapy, where surface topography during bioreactor expansion should be taken into account to preserve therapeutic properties.Daniëlle G. LeuningNick R. M. BeijerNadia A. du FosséSteven VermeulenEllen LieversCees van KootenTon J. RabelinkJan de BoerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daniëlle G. Leuning Nick R. M. Beijer Nadia A. du Fossé Steven Vermeulen Ellen Lievers Cees van Kooten Ton J. Rabelink Jan de Boer The cytokine secretion profile of mesenchymal stromal cells is determined by surface structure of the microenvironment
description	Abstract Mesenchymal stromal cells (MSC) secrete factors that contribute to organ homeostasis and repair in a tissue specific manner. For instance, kidney perivascular mesenchymal stromal cells (kPSCs) can facilitate renal epithelial repair through secretion of hepatocyte growth factor (HGF) while the secretome of bone marrow MSCs gives rise to immunosuppression. Stromal cells function in a complex 3-dimensional (3D) connective tissue architecture that induces conformational adaptation. Here we tested the hypothesis that surface topography and associated cell adaptations dictate stromal cell function through tuning of the cytokines released. To this end, we cultured human bone marrow and kidney perivascular stromal cells in the TopoWell plate, a custom-fabricated multi-well plate containing 76 unique bioactive surface topographies. Using fluorescent imaging, we observed profound changes in cell shape, accompanied by major quantitative changes in the secretory capacity of the MSCs. The cytokine secretion profile was closely related to cell morphology and was stromal cell type specific. Our data demonstrate that stromal cell function is determined by microenvironment structure and can be manipulated in an engineered setting. Our data also have implications for the clinical manufacturing of mesenchymal stromal cell therapy, where surface topography during bioreactor expansion should be taken into account to preserve therapeutic properties.
format	article
author	Daniëlle G. Leuning Nick R. M. Beijer Nadia A. du Fossé Steven Vermeulen Ellen Lievers Cees van Kooten Ton J. Rabelink Jan de Boer
author_facet	Daniëlle G. Leuning Nick R. M. Beijer Nadia A. du Fossé Steven Vermeulen Ellen Lievers Cees van Kooten Ton J. Rabelink Jan de Boer
author_sort	Daniëlle G. Leuning
title	The cytokine secretion profile of mesenchymal stromal cells is determined by surface structure of the microenvironment
title_short	The cytokine secretion profile of mesenchymal stromal cells is determined by surface structure of the microenvironment
title_full	The cytokine secretion profile of mesenchymal stromal cells is determined by surface structure of the microenvironment
title_fullStr	The cytokine secretion profile of mesenchymal stromal cells is determined by surface structure of the microenvironment
title_full_unstemmed	The cytokine secretion profile of mesenchymal stromal cells is determined by surface structure of the microenvironment
title_sort	cytokine secretion profile of mesenchymal stromal cells is determined by surface structure of the microenvironment
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/f23b075466864c9d9975c16924bc8abe
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The cytokine secretion profile of mesenchymal stromal cells is determined by surface structure of the microenvironment

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