Molecular Evaluation of High Fluoroquinolone Resistant Genes in Endemic Cases of Shigellosis, Northeast Part of Karnataka, India

<p>Objectives</p><p>Shigellosis is an acute infection of the intestine caused by bacteria in the genus 'Shigella' and also an important cause of diarrhea in developing countries. This study was carried out to find the extent and nature of the emerging resistance in north...

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Autores principales:	Prabhurajeshwar C, Pramod Desai, Kelmani Chandrakanth R
Formato:	article
Lenguaje:	EN
Publicado:	Ubiquity Press 2017
Materias:	Shigella species minimal inhibitory concentration (MICs) fluoroquinolone genes QRDRs gyrA gyrB parC and pare genomic DNA PCR amplification restriction digestion and shigellosis Infectious and parasitic diseases RC109-216 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/f23f28451f2845629007418f7ee49ad2
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spelling	oai:doaj.org-article:f23f28451f2845629007418f7ee49ad22021-12-02T07:30:14ZMolecular Evaluation of High Fluoroquinolone Resistant Genes in Endemic Cases of Shigellosis, Northeast Part of Karnataka, India2214-999610.1016/j.aogh.2016.09.009https://doaj.org/article/f23f28451f2845629007418f7ee49ad22017-03-01T00:00:00Zhttps://annalsofglobalhealth.org/articles/711https://doaj.org/toc/2214-9996<p>Objectives</p><p>Shigellosis is an acute infection of the intestine caused by bacteria in the genus 'Shigella' and also an important cause of diarrhea in developing countries. This study was carried out to find the extent and nature of the emerging resistance in north part of Karnataka, India, and surrounding region with huge population, and also focused on the molecular mechanism of development of resistance against different generations of fluoroquinolones and explored the diversity of restriction endonucleases; we also tried to establish the significance of reduced minimal inhibitory concentrations (MIC) values.</p><p>Methods</p><p>A total of 32 multidrug-resistant 'Shigella' species (isolated from infants’ stools) were subjected to MICs of fluoroquinolone-resistant isolates done by both broth dilution and E-test method. The genes implicated in resistance to fluoroquinolone generations ciprofloxacin, ofloxacin, and gatifloxacin (gyrA, gyrB, parC, and parE) were amplified using polymerase chain reaction (PCR) method and restriction digestion analysis of PCR product were performed using PvuI and HaeII enzymes.</p><p>Findings</p><p>Fluoroquinolone-resistant 'Shigella' species (n = 32) comprising 'S dysenteriae', 'S flexneri', and 'S sonnei' were selected for MIC; 90.6% (29/32), 93.75% (30/32), and 93.75% (30/32) of isolates were ciprofloxacin, ofloxacin, and gatifloxacin resistant and showed the MIC range from 4-128 μg/mL. The PCR amplification results were positive for all species and asserted the presence of gyrA, gyrB, parC, and pare and sizes of the amplified products. The restriction banding patterns of amplified resistant genes were employed to detect differences among the 'Shigella' species.</p><p>Conclusions</p><p>The present study found that the genetic basis and its characterization of fluoroquinolone resistance in 'Shigella' isolates was considered for the common resistant genes, namely, gyrA, gyrB, parC, and pare, and had mutations at position 83 of gyrA and at position 80 of parC of the quinolone-resistant determining regions and associated molecular mechanism. Our study beneficial in identification of the causative agents of the infections, careful control and cautions use of antibiotics must be promoted, particularly to monitor the emergence of isolates that are fully resistant to fluoroquinolones.Prabhurajeshwar CPramod DesaiKelmani Chandrakanth RUbiquity PressarticleShigella speciesminimal inhibitory concentration (MICs)fluoroquinolone genesQRDRsgyrAgyrBparCand paregenomic DNAPCR amplificationrestriction digestion and shigellosisInfectious and parasitic diseasesRC109-216Public aspects of medicineRA1-1270ENAnnals of Global Health, Vol 82, Iss 5, Pp 832-839 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Shigella species minimal inhibitory concentration (MICs) fluoroquinolone genes QRDRs gyrA gyrB parC and pare genomic DNA PCR amplification restriction digestion and shigellosis Infectious and parasitic diseases RC109-216 Public aspects of medicine RA1-1270
spellingShingle	Shigella species minimal inhibitory concentration (MICs) fluoroquinolone genes QRDRs gyrA gyrB parC and pare genomic DNA PCR amplification restriction digestion and shigellosis Infectious and parasitic diseases RC109-216 Public aspects of medicine RA1-1270 Prabhurajeshwar C Pramod Desai Kelmani Chandrakanth R Molecular Evaluation of High Fluoroquinolone Resistant Genes in Endemic Cases of Shigellosis, Northeast Part of Karnataka, India
description	<p>Objectives</p><p>Shigellosis is an acute infection of the intestine caused by bacteria in the genus 'Shigella' and also an important cause of diarrhea in developing countries. This study was carried out to find the extent and nature of the emerging resistance in north part of Karnataka, India, and surrounding region with huge population, and also focused on the molecular mechanism of development of resistance against different generations of fluoroquinolones and explored the diversity of restriction endonucleases; we also tried to establish the significance of reduced minimal inhibitory concentrations (MIC) values.</p><p>Methods</p><p>A total of 32 multidrug-resistant 'Shigella' species (isolated from infants’ stools) were subjected to MICs of fluoroquinolone-resistant isolates done by both broth dilution and E-test method. The genes implicated in resistance to fluoroquinolone generations ciprofloxacin, ofloxacin, and gatifloxacin (gyrA, gyrB, parC, and parE) were amplified using polymerase chain reaction (PCR) method and restriction digestion analysis of PCR product were performed using PvuI and HaeII enzymes.</p><p>Findings</p><p>Fluoroquinolone-resistant 'Shigella' species (n = 32) comprising 'S dysenteriae', 'S flexneri', and 'S sonnei' were selected for MIC; 90.6% (29/32), 93.75% (30/32), and 93.75% (30/32) of isolates were ciprofloxacin, ofloxacin, and gatifloxacin resistant and showed the MIC range from 4-128 μg/mL. The PCR amplification results were positive for all species and asserted the presence of gyrA, gyrB, parC, and pare and sizes of the amplified products. The restriction banding patterns of amplified resistant genes were employed to detect differences among the 'Shigella' species.</p><p>Conclusions</p><p>The present study found that the genetic basis and its characterization of fluoroquinolone resistance in 'Shigella' isolates was considered for the common resistant genes, namely, gyrA, gyrB, parC, and pare, and had mutations at position 83 of gyrA and at position 80 of parC of the quinolone-resistant determining regions and associated molecular mechanism. Our study beneficial in identification of the causative agents of the infections, careful control and cautions use of antibiotics must be promoted, particularly to monitor the emergence of isolates that are fully resistant to fluoroquinolones.
format	article
author	Prabhurajeshwar C Pramod Desai Kelmani Chandrakanth R
author_facet	Prabhurajeshwar C Pramod Desai Kelmani Chandrakanth R
author_sort	Prabhurajeshwar C
title	Molecular Evaluation of High Fluoroquinolone Resistant Genes in Endemic Cases of Shigellosis, Northeast Part of Karnataka, India
title_short	Molecular Evaluation of High Fluoroquinolone Resistant Genes in Endemic Cases of Shigellosis, Northeast Part of Karnataka, India
title_full	Molecular Evaluation of High Fluoroquinolone Resistant Genes in Endemic Cases of Shigellosis, Northeast Part of Karnataka, India
title_fullStr	Molecular Evaluation of High Fluoroquinolone Resistant Genes in Endemic Cases of Shigellosis, Northeast Part of Karnataka, India
title_full_unstemmed	Molecular Evaluation of High Fluoroquinolone Resistant Genes in Endemic Cases of Shigellosis, Northeast Part of Karnataka, India
title_sort	molecular evaluation of high fluoroquinolone resistant genes in endemic cases of shigellosis, northeast part of karnataka, india
publisher	Ubiquity Press
publishDate	2017
url	https://doaj.org/article/f23f28451f2845629007418f7ee49ad2
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_version_	1718399436203229184

Molecular Evaluation of High Fluoroquinolone Resistant Genes in Endemic Cases of Shigellosis, Northeast Part of Karnataka, India

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