A sensitive mass spectrometry platform identifies metabolic changes of life history traits in C. elegans

Abstract Abnormal nutrient metabolism is a hallmark of aging, and the underlying genetic and nutritional framework is rapidly being uncovered, particularly using C. elegans as a model. However, the direct metabolic consequences of perturbations in life history of C. elegans remain to be clarified. B...

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Autores principales:	Arwen W. Gao, Iliana A. Chatzispyrou, Rashmi Kamble, Yasmine J. Liu, Katharina Herzog, Reuben L. Smith, Henk van Lenthe, Martin A. T. Vervaart, Arno van Cruchten, Angela C. Luyf, Antoine van Kampen, Mia L. Pras-Raves, Frédéric M. Vaz, Riekelt H. Houtkooper
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f244d9a20b94447d85bf084d64d7035a
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spelling	oai:doaj.org-article:f244d9a20b94447d85bf084d64d7035a2021-12-02T11:41:10ZA sensitive mass spectrometry platform identifies metabolic changes of life history traits in C. elegans10.1038/s41598-017-02539-w2045-2322https://doaj.org/article/f244d9a20b94447d85bf084d64d7035a2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02539-whttps://doaj.org/toc/2045-2322Abstract Abnormal nutrient metabolism is a hallmark of aging, and the underlying genetic and nutritional framework is rapidly being uncovered, particularly using C. elegans as a model. However, the direct metabolic consequences of perturbations in life history of C. elegans remain to be clarified. Based on recent advances in the metabolomics field, we optimized and validated a sensitive mass spectrometry (MS) platform for identification of major metabolite classes in worms and applied it to study age and diet related changes. Using this platform that allowed detection of over 600 metabolites in a sample of 2500 worms, we observed marked changes in fatty acids, amino acids and phospholipids during worm life history, which were independent from the germ-line. Worms underwent a striking shift in lipid metabolism after early adulthood that was at least partly controlled by the metabolic regulator AAK-2/AMPK. Most amino acids peaked during development, except aspartic acid and glycine, which accumulated in aged worms. Dietary intervention also influenced worm metabolite profiles and the regulation was highly specific depending on the metabolite class. Altogether, these MS-based methods are powerful tools to perform worm metabolomics for aging and metabolism-oriented studies.Arwen W. GaoIliana A. ChatzispyrouRashmi KambleYasmine J. LiuKatharina HerzogReuben L. SmithHenk van LentheMartin A. T. VervaartArno van CruchtenAngela C. LuyfAntoine van KampenMia L. Pras-RavesFrédéric M. VazRiekelt H. HoutkooperNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Arwen W. Gao Iliana A. Chatzispyrou Rashmi Kamble Yasmine J. Liu Katharina Herzog Reuben L. Smith Henk van Lenthe Martin A. T. Vervaart Arno van Cruchten Angela C. Luyf Antoine van Kampen Mia L. Pras-Raves Frédéric M. Vaz Riekelt H. Houtkooper A sensitive mass spectrometry platform identifies metabolic changes of life history traits in C. elegans
description	Abstract Abnormal nutrient metabolism is a hallmark of aging, and the underlying genetic and nutritional framework is rapidly being uncovered, particularly using C. elegans as a model. However, the direct metabolic consequences of perturbations in life history of C. elegans remain to be clarified. Based on recent advances in the metabolomics field, we optimized and validated a sensitive mass spectrometry (MS) platform for identification of major metabolite classes in worms and applied it to study age and diet related changes. Using this platform that allowed detection of over 600 metabolites in a sample of 2500 worms, we observed marked changes in fatty acids, amino acids and phospholipids during worm life history, which were independent from the germ-line. Worms underwent a striking shift in lipid metabolism after early adulthood that was at least partly controlled by the metabolic regulator AAK-2/AMPK. Most amino acids peaked during development, except aspartic acid and glycine, which accumulated in aged worms. Dietary intervention also influenced worm metabolite profiles and the regulation was highly specific depending on the metabolite class. Altogether, these MS-based methods are powerful tools to perform worm metabolomics for aging and metabolism-oriented studies.
format	article
author	Arwen W. Gao Iliana A. Chatzispyrou Rashmi Kamble Yasmine J. Liu Katharina Herzog Reuben L. Smith Henk van Lenthe Martin A. T. Vervaart Arno van Cruchten Angela C. Luyf Antoine van Kampen Mia L. Pras-Raves Frédéric M. Vaz Riekelt H. Houtkooper
author_facet	Arwen W. Gao Iliana A. Chatzispyrou Rashmi Kamble Yasmine J. Liu Katharina Herzog Reuben L. Smith Henk van Lenthe Martin A. T. Vervaart Arno van Cruchten Angela C. Luyf Antoine van Kampen Mia L. Pras-Raves Frédéric M. Vaz Riekelt H. Houtkooper
author_sort	Arwen W. Gao
title	A sensitive mass spectrometry platform identifies metabolic changes of life history traits in C. elegans
title_short	A sensitive mass spectrometry platform identifies metabolic changes of life history traits in C. elegans
title_full	A sensitive mass spectrometry platform identifies metabolic changes of life history traits in C. elegans
title_fullStr	A sensitive mass spectrometry platform identifies metabolic changes of life history traits in C. elegans
title_full_unstemmed	A sensitive mass spectrometry platform identifies metabolic changes of life history traits in C. elegans
title_sort	sensitive mass spectrometry platform identifies metabolic changes of life history traits in c. elegans
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f244d9a20b94447d85bf084d64d7035a
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A sensitive mass spectrometry platform identifies metabolic changes of life history traits in C. elegans

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