Interspecies and intraspecies genetic diversity of Ongole Grade cattle and Madura cattle based on microsatellite DNA markers

Sutarno, Kurnianingrum N, Herawati E, Setyawan AD. 2018. Interspecies and intraspecies genetic diversity of Ongole Grade cattle and Madura cattle based on microsatellite DNA markers. Biodiversitas 19: 1593-1600. DNA microsatellite has been extensively employed for estimating the degree of kinship be...

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Autores principales:	SUTARNO SUTARNO, NINA KURNIANINGRUM, ELISA HERAWATI, AHMAD DWI SETYAWAN
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2018
Materias:	ongole grade cattle madura cattle genetic diversity dna microsatellite Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f24daab23d764f64abc12452c601e267
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spelling	oai:doaj.org-article:f24daab23d764f64abc12452c601e2672021-11-16T14:03:29ZInterspecies and intraspecies genetic diversity of Ongole Grade cattle and Madura cattle based on microsatellite DNA markers1412-033X2085-472210.13057/biodiv/d190453https://doaj.org/article/f24daab23d764f64abc12452c601e2672018-07-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2891https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Sutarno, Kurnianingrum N, Herawati E, Setyawan AD. 2018. Interspecies and intraspecies genetic diversity of Ongole Grade cattle and Madura cattle based on microsatellite DNA markers. Biodiversitas 19: 1593-1600. DNA microsatellite has been extensively employed for estimating the degree of kinship between genotypes and improving the quality of cattle products. Microsatellite markers are short-patterned DNA sequences and repeated tandem (sequentially) with 2-5 nucleotide units scattering the entire genome. The purpose of this study was to investigate the genetic characteristics of inter and intraspecies of Ongole Grade cattle and Madura cattle using microsatellite DNA markers. Blood samples from 20 individuals of each species were extracted by the method referring to Wizard Genomic DNA Purification Kit (Promega, USA) and PCR amplification was performed using 5 microsatellite loci, i.e., BM1824, ETH225, INRA005, MM12, and TGLA227. Results of the genetic characteristics of both species were calculated using the POPGENE program version 1.31. The data suggest that there is a genetic diversity of inter and intraspecies of Ongole Grade cattle and Madura cattle. The average value of Shannon's Information Index (I) at all microsatellite loci for Ongole Grade cattle was 0.76 and for Madura cattle was 1.12. Meanwhile, the average interspecies I value was 1.03. The mean intraspecies Polymorphic Information Content (PIC) of Ongole Grade and Madura cattle was 0.43, and 0.63, respectively, and the mean interspecies PIC value was 0.57. The data altogether suggest that all loci meet the standards as being informative markers in the assessment of genetic population because it has a PIC value> 0.5 especially for intraspecies of Madura cattle.SUTARNO SUTARNONINA KURNIANINGRUMELISA HERAWATIAHMAD DWI SETYAWANMBI & UNS Soloarticleongolegrade cattlemadura cattlegenetic diversitydna microsatelliteBiology (General)QH301-705.5ENBiodiversitas, Vol 19, Iss 4, Pp 1593-1600 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	ongole grade cattle madura cattle genetic diversity dna microsatellite Biology (General) QH301-705.5
spellingShingle	ongole grade cattle madura cattle genetic diversity dna microsatellite Biology (General) QH301-705.5 SUTARNO SUTARNO NINA KURNIANINGRUM ELISA HERAWATI AHMAD DWI SETYAWAN Interspecies and intraspecies genetic diversity of Ongole Grade cattle and Madura cattle based on microsatellite DNA markers
description	Sutarno, Kurnianingrum N, Herawati E, Setyawan AD. 2018. Interspecies and intraspecies genetic diversity of Ongole Grade cattle and Madura cattle based on microsatellite DNA markers. Biodiversitas 19: 1593-1600. DNA microsatellite has been extensively employed for estimating the degree of kinship between genotypes and improving the quality of cattle products. Microsatellite markers are short-patterned DNA sequences and repeated tandem (sequentially) with 2-5 nucleotide units scattering the entire genome. The purpose of this study was to investigate the genetic characteristics of inter and intraspecies of Ongole Grade cattle and Madura cattle using microsatellite DNA markers. Blood samples from 20 individuals of each species were extracted by the method referring to Wizard Genomic DNA Purification Kit (Promega, USA) and PCR amplification was performed using 5 microsatellite loci, i.e., BM1824, ETH225, INRA005, MM12, and TGLA227. Results of the genetic characteristics of both species were calculated using the POPGENE program version 1.31. The data suggest that there is a genetic diversity of inter and intraspecies of Ongole Grade cattle and Madura cattle. The average value of Shannon's Information Index (I) at all microsatellite loci for Ongole Grade cattle was 0.76 and for Madura cattle was 1.12. Meanwhile, the average interspecies I value was 1.03. The mean intraspecies Polymorphic Information Content (PIC) of Ongole Grade and Madura cattle was 0.43, and 0.63, respectively, and the mean interspecies PIC value was 0.57. The data altogether suggest that all loci meet the standards as being informative markers in the assessment of genetic population because it has a PIC value> 0.5 especially for intraspecies of Madura cattle.
format	article
author	SUTARNO SUTARNO NINA KURNIANINGRUM ELISA HERAWATI AHMAD DWI SETYAWAN
author_facet	SUTARNO SUTARNO NINA KURNIANINGRUM ELISA HERAWATI AHMAD DWI SETYAWAN
author_sort	SUTARNO SUTARNO
title	Interspecies and intraspecies genetic diversity of Ongole Grade cattle and Madura cattle based on microsatellite DNA markers
title_short	Interspecies and intraspecies genetic diversity of Ongole Grade cattle and Madura cattle based on microsatellite DNA markers
title_full	Interspecies and intraspecies genetic diversity of Ongole Grade cattle and Madura cattle based on microsatellite DNA markers
title_fullStr	Interspecies and intraspecies genetic diversity of Ongole Grade cattle and Madura cattle based on microsatellite DNA markers
title_full_unstemmed	Interspecies and intraspecies genetic diversity of Ongole Grade cattle and Madura cattle based on microsatellite DNA markers
title_sort	interspecies and intraspecies genetic diversity of ongole grade cattle and madura cattle based on microsatellite dna markers
publisher	MBI & UNS Solo
publishDate	2018
url	https://doaj.org/article/f24daab23d764f64abc12452c601e267
work_keys_str_mv	AT sutarnosutarno interspeciesandintraspeciesgeneticdiversityofongolegradecattleandmaduracattlebasedonmicrosatellitednamarkers AT ninakurnianingrum interspeciesandintraspeciesgeneticdiversityofongolegradecattleandmaduracattlebasedonmicrosatellitednamarkers AT elisaherawati interspeciesandintraspeciesgeneticdiversityofongolegradecattleandmaduracattlebasedonmicrosatellitednamarkers AT ahmaddwisetyawan interspeciesandintraspeciesgeneticdiversityofongolegradecattleandmaduracattlebasedonmicrosatellitednamarkers
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Interspecies and intraspecies genetic diversity of Ongole Grade cattle and Madura cattle based on microsatellite DNA markers

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