Leptotene/zygotene chromosome movement via the SUN/KASH protein bridge in Caenorhabditis elegans.

The Caenorhabditis elegans inner nuclear envelope protein matefin/SUN-1 plays a conserved, pivotal role in the process of genome haploidization. CHK-2-dependent phosphorylation of SUN-1 regulates homologous chromosome pairing and interhomolog recombination in Caenorhabditis elegans. Using time-lapse...

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Autores principales:	Antoine Baudrimont, Alexandra Penkner, Alexander Woglar, Thomas Machacek, Christina Wegrostek, Jiradet Gloggnitzer, Alexandra Fridkin, Franz Klein, Yosef Gruenbaum, Pawel Pasierbek, Verena Jantsch
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/f2504971f4304d31a8a4a109e5013086
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spelling	oai:doaj.org-article:f2504971f4304d31a8a4a109e50130862021-11-18T06:19:18ZLeptotene/zygotene chromosome movement via the SUN/KASH protein bridge in Caenorhabditis elegans.1553-73901553-740410.1371/journal.pgen.1001219https://doaj.org/article/f2504971f4304d31a8a4a109e50130862010-11-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21124819/?tool=EBIhttps://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404The Caenorhabditis elegans inner nuclear envelope protein matefin/SUN-1 plays a conserved, pivotal role in the process of genome haploidization. CHK-2-dependent phosphorylation of SUN-1 regulates homologous chromosome pairing and interhomolog recombination in Caenorhabditis elegans. Using time-lapse microscopy, we characterized the movement of matefin/SUN-1::GFP aggregates (the equivalent of chromosomal attachment plaques) and showed that the dynamics of matefin/SUN-1 aggregates remained unchanged throughout leptonene/zygotene, despite the progression of pairing. Movement of SUN-1 aggregates correlated with chromatin polarization. We also analyzed the requirements for the formation of movement-competent matefin/SUN-1 aggregates in the context of chromosome structure and found that chromosome axes were required to produce wild-type numbers of attachment plaques. Abrogation of synapsis led to a deceleration of SUN-1 aggregate movement. Analysis of matefin/SUN-1 in a double-strand break deficient mutant revealed that repair intermediates influenced matefin/SUN-1 aggregate dynamics. Investigation of movement in meiotic regulator mutants substantiated that proper orchestration of the meiotic program and effective repair of DNA double-strand breaks were necessary for the wild-type behavior of matefin/SUN-1 aggregates.Antoine BaudrimontAlexandra PenknerAlexander WoglarThomas MachacekChristina WegrostekJiradet GloggnitzerAlexandra FridkinFranz KleinYosef GruenbaumPawel PasierbekVerena JantschPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 6, Iss 11, p e1001219 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Antoine Baudrimont Alexandra Penkner Alexander Woglar Thomas Machacek Christina Wegrostek Jiradet Gloggnitzer Alexandra Fridkin Franz Klein Yosef Gruenbaum Pawel Pasierbek Verena Jantsch Leptotene/zygotene chromosome movement via the SUN/KASH protein bridge in Caenorhabditis elegans.
description	The Caenorhabditis elegans inner nuclear envelope protein matefin/SUN-1 plays a conserved, pivotal role in the process of genome haploidization. CHK-2-dependent phosphorylation of SUN-1 regulates homologous chromosome pairing and interhomolog recombination in Caenorhabditis elegans. Using time-lapse microscopy, we characterized the movement of matefin/SUN-1::GFP aggregates (the equivalent of chromosomal attachment plaques) and showed that the dynamics of matefin/SUN-1 aggregates remained unchanged throughout leptonene/zygotene, despite the progression of pairing. Movement of SUN-1 aggregates correlated with chromatin polarization. We also analyzed the requirements for the formation of movement-competent matefin/SUN-1 aggregates in the context of chromosome structure and found that chromosome axes were required to produce wild-type numbers of attachment plaques. Abrogation of synapsis led to a deceleration of SUN-1 aggregate movement. Analysis of matefin/SUN-1 in a double-strand break deficient mutant revealed that repair intermediates influenced matefin/SUN-1 aggregate dynamics. Investigation of movement in meiotic regulator mutants substantiated that proper orchestration of the meiotic program and effective repair of DNA double-strand breaks were necessary for the wild-type behavior of matefin/SUN-1 aggregates.
format	article
author	Antoine Baudrimont Alexandra Penkner Alexander Woglar Thomas Machacek Christina Wegrostek Jiradet Gloggnitzer Alexandra Fridkin Franz Klein Yosef Gruenbaum Pawel Pasierbek Verena Jantsch
author_facet	Antoine Baudrimont Alexandra Penkner Alexander Woglar Thomas Machacek Christina Wegrostek Jiradet Gloggnitzer Alexandra Fridkin Franz Klein Yosef Gruenbaum Pawel Pasierbek Verena Jantsch
author_sort	Antoine Baudrimont
title	Leptotene/zygotene chromosome movement via the SUN/KASH protein bridge in Caenorhabditis elegans.
title_short	Leptotene/zygotene chromosome movement via the SUN/KASH protein bridge in Caenorhabditis elegans.
title_full	Leptotene/zygotene chromosome movement via the SUN/KASH protein bridge in Caenorhabditis elegans.
title_fullStr	Leptotene/zygotene chromosome movement via the SUN/KASH protein bridge in Caenorhabditis elegans.
title_full_unstemmed	Leptotene/zygotene chromosome movement via the SUN/KASH protein bridge in Caenorhabditis elegans.
title_sort	leptotene/zygotene chromosome movement via the sun/kash protein bridge in caenorhabditis elegans.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/f2504971f4304d31a8a4a109e5013086
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Leptotene/zygotene chromosome movement via the SUN/KASH protein bridge in Caenorhabditis elegans.

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