Multiple particle tracking analysis in isolated nuclei reveals the mechanical phenotype of leukemia cells

Abstract The nucleus is fundamentally composed by lamina and nuclear membranes that enclose the chromatin, nucleoskeletal components and suspending nucleoplasm. The functional connections of this network integrate external stimuli into cell signals, including physical forces to mechanical responses...

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Autores principales:	Diego Herráez-Aguilar, Elena Madrazo, Horacio López-Menéndez, Manuel Ramírez, Francisco Monroy, Javier Redondo-Muñoz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f251fc62450d4b088f3d2148df2eeecc
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spelling	oai:doaj.org-article:f251fc62450d4b088f3d2148df2eeecc2021-12-02T18:27:50ZMultiple particle tracking analysis in isolated nuclei reveals the mechanical phenotype of leukemia cells10.1038/s41598-020-63682-52045-2322https://doaj.org/article/f251fc62450d4b088f3d2148df2eeecc2020-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-63682-5https://doaj.org/toc/2045-2322Abstract The nucleus is fundamentally composed by lamina and nuclear membranes that enclose the chromatin, nucleoskeletal components and suspending nucleoplasm. The functional connections of this network integrate external stimuli into cell signals, including physical forces to mechanical responses of the nucleus. Canonically, the morphological characteristics of the nucleus, as shape and size, have served for pathologists to stratify and diagnose cancer patients; however, novel biophysical techniques must exploit physical parameters to improve cancer diagnosis. By using multiple particle tracking (MPT) technique on chromatin granules, we designed a SURF (Speeded Up Robust Features)-based algorithm to study the mechanical properties of isolated nuclei and in living cells. We have determined the apparent shear stiffness, viscosity and optical density of the nucleus, and how the chromatin structure influences on these biophysical values. Moreover, we used our MPT-SURF analysis to study the apparent mechanical properties of isolated nuclei from patients of acute lymphoblastic leukemia. We found that leukemia cells exhibited mechanical differences compared to normal lymphocytes. Interestingly, isolated nuclei from high-risk leukemia cells showed increased viscosity than their counterparts from normal lymphocytes, whilst nuclei from relapsed-patient's cells presented higher density than those from normal lymphocytes or standard- and high-risk leukemia cells. Taken together, here we presented how MPT-SURF analysis of nuclear chromatin granules defines nuclear mechanical phenotypic features, which might be clinically relevant.Diego Herráez-AguilarElena MadrazoHoracio López-MenéndezManuel RamírezFrancisco MonroyJavier Redondo-MuñozNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Diego Herráez-Aguilar Elena Madrazo Horacio López-Menéndez Manuel Ramírez Francisco Monroy Javier Redondo-Muñoz Multiple particle tracking analysis in isolated nuclei reveals the mechanical phenotype of leukemia cells
description	Abstract The nucleus is fundamentally composed by lamina and nuclear membranes that enclose the chromatin, nucleoskeletal components and suspending nucleoplasm. The functional connections of this network integrate external stimuli into cell signals, including physical forces to mechanical responses of the nucleus. Canonically, the morphological characteristics of the nucleus, as shape and size, have served for pathologists to stratify and diagnose cancer patients; however, novel biophysical techniques must exploit physical parameters to improve cancer diagnosis. By using multiple particle tracking (MPT) technique on chromatin granules, we designed a SURF (Speeded Up Robust Features)-based algorithm to study the mechanical properties of isolated nuclei and in living cells. We have determined the apparent shear stiffness, viscosity and optical density of the nucleus, and how the chromatin structure influences on these biophysical values. Moreover, we used our MPT-SURF analysis to study the apparent mechanical properties of isolated nuclei from patients of acute lymphoblastic leukemia. We found that leukemia cells exhibited mechanical differences compared to normal lymphocytes. Interestingly, isolated nuclei from high-risk leukemia cells showed increased viscosity than their counterparts from normal lymphocytes, whilst nuclei from relapsed-patient's cells presented higher density than those from normal lymphocytes or standard- and high-risk leukemia cells. Taken together, here we presented how MPT-SURF analysis of nuclear chromatin granules defines nuclear mechanical phenotypic features, which might be clinically relevant.
format	article
author	Diego Herráez-Aguilar Elena Madrazo Horacio López-Menéndez Manuel Ramírez Francisco Monroy Javier Redondo-Muñoz
author_facet	Diego Herráez-Aguilar Elena Madrazo Horacio López-Menéndez Manuel Ramírez Francisco Monroy Javier Redondo-Muñoz
author_sort	Diego Herráez-Aguilar
title	Multiple particle tracking analysis in isolated nuclei reveals the mechanical phenotype of leukemia cells
title_short	Multiple particle tracking analysis in isolated nuclei reveals the mechanical phenotype of leukemia cells
title_full	Multiple particle tracking analysis in isolated nuclei reveals the mechanical phenotype of leukemia cells
title_fullStr	Multiple particle tracking analysis in isolated nuclei reveals the mechanical phenotype of leukemia cells
title_full_unstemmed	Multiple particle tracking analysis in isolated nuclei reveals the mechanical phenotype of leukemia cells
title_sort	multiple particle tracking analysis in isolated nuclei reveals the mechanical phenotype of leukemia cells
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/f251fc62450d4b088f3d2148df2eeecc
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Multiple particle tracking analysis in isolated nuclei reveals the mechanical phenotype of leukemia cells

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