Common oncogenic mutations are infrequent in oral squamous cell carcinoma of Asian origin.

<h4>Objectives</h4>The frequency of common oncogenic mutations and TP53 was determined in Asian oral squamous cell carcinoma (OSCC).<h4>Materials and methods</h4>The OncoCarta(™) panel v1.0 assay was used to characterize oncogenic mutations. In addition, exons 4-11 of the TP5...

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Autores principales:	Sharifah Nurain Syed Zanaruddin, Pei San Yee, Seen Yii Hor, Yink Heay Kong, Wan Maria Nabillah Wan Abd Ghani, Wan Mahadzir Wan Mustafa, Rosnah Binti Zain, Stephen S Prime, Zainal Ariff Abd Rahman, Sok-Ching Cheong
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f2520dd8911c4275bef15354eea1df31
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spelling	oai:doaj.org-article:f2520dd8911c4275bef15354eea1df312021-11-18T08:48:24ZCommon oncogenic mutations are infrequent in oral squamous cell carcinoma of Asian origin.1932-620310.1371/journal.pone.0080229https://doaj.org/article/f2520dd8911c4275bef15354eea1df312013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24224046/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Objectives</h4>The frequency of common oncogenic mutations and TP53 was determined in Asian oral squamous cell carcinoma (OSCC).<h4>Materials and methods</h4>The OncoCarta(™) panel v1.0 assay was used to characterize oncogenic mutations. In addition, exons 4-11 of the TP53 gene were sequenced. Statistical analyses were conducted to identify associations between mutations and selected clinico-pathological characteristics and risk habits.<h4>Results</h4>Oncogenic mutations were detected in PIK3CA (5.7%) and HRAS (2.4%). Mutations in TP53 were observed in 27.7% (31/112) of the OSCC specimens. Oncogenic mutations were found more frequently in non-smokers (p = 0.049) and TP53 truncating mutations were more common in patients with no risk habits (p = 0.019). Patients with mutations had worse overall survival compared to those with absence of mutations; and patients who harbored DNA binding domain (DBD) and L2/L3/LSH mutations showed a worse survival probability compared to those patients with wild type TP53. The majority of the oncogenic and TP53 mutations were G:C > A:T and A:T > G:C base transitions, regardless of the different risk habits.<h4>Conclusion</h4>Hotspot oncogenic mutations which are frequently present in common solid tumors are exceedingly rare in OSCC. Despite differences in risk habit exposure, the mutation frequency of PIK3CA and HRAS in Asian OSCC were similar to that reported in OSCC among Caucasians, whereas TP53 mutations rates were significantly lower. The lack of actionable hotspot mutations argue strongly for the need to comprehensively characterize gene mutations associated with OSCC for the development of new diagnostic and therapeutic tools.Sharifah Nurain Syed ZanaruddinPei San YeeSeen Yii HorYink Heay KongWan Maria Nabillah Wan Abd GhaniWan Mahadzir Wan MustafaRosnah Binti ZainStephen S PrimeZainal Ariff Abd RahmanSok-Ching CheongPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e80229 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sharifah Nurain Syed Zanaruddin Pei San Yee Seen Yii Hor Yink Heay Kong Wan Maria Nabillah Wan Abd Ghani Wan Mahadzir Wan Mustafa Rosnah Binti Zain Stephen S Prime Zainal Ariff Abd Rahman Sok-Ching Cheong Common oncogenic mutations are infrequent in oral squamous cell carcinoma of Asian origin.
description	<h4>Objectives</h4>The frequency of common oncogenic mutations and TP53 was determined in Asian oral squamous cell carcinoma (OSCC).<h4>Materials and methods</h4>The OncoCarta(™) panel v1.0 assay was used to characterize oncogenic mutations. In addition, exons 4-11 of the TP53 gene were sequenced. Statistical analyses were conducted to identify associations between mutations and selected clinico-pathological characteristics and risk habits.<h4>Results</h4>Oncogenic mutations were detected in PIK3CA (5.7%) and HRAS (2.4%). Mutations in TP53 were observed in 27.7% (31/112) of the OSCC specimens. Oncogenic mutations were found more frequently in non-smokers (p = 0.049) and TP53 truncating mutations were more common in patients with no risk habits (p = 0.019). Patients with mutations had worse overall survival compared to those with absence of mutations; and patients who harbored DNA binding domain (DBD) and L2/L3/LSH mutations showed a worse survival probability compared to those patients with wild type TP53. The majority of the oncogenic and TP53 mutations were G:C > A:T and A:T > G:C base transitions, regardless of the different risk habits.<h4>Conclusion</h4>Hotspot oncogenic mutations which are frequently present in common solid tumors are exceedingly rare in OSCC. Despite differences in risk habit exposure, the mutation frequency of PIK3CA and HRAS in Asian OSCC were similar to that reported in OSCC among Caucasians, whereas TP53 mutations rates were significantly lower. The lack of actionable hotspot mutations argue strongly for the need to comprehensively characterize gene mutations associated with OSCC for the development of new diagnostic and therapeutic tools.
format	article
author	Sharifah Nurain Syed Zanaruddin Pei San Yee Seen Yii Hor Yink Heay Kong Wan Maria Nabillah Wan Abd Ghani Wan Mahadzir Wan Mustafa Rosnah Binti Zain Stephen S Prime Zainal Ariff Abd Rahman Sok-Ching Cheong
author_facet	Sharifah Nurain Syed Zanaruddin Pei San Yee Seen Yii Hor Yink Heay Kong Wan Maria Nabillah Wan Abd Ghani Wan Mahadzir Wan Mustafa Rosnah Binti Zain Stephen S Prime Zainal Ariff Abd Rahman Sok-Ching Cheong
author_sort	Sharifah Nurain Syed Zanaruddin
title	Common oncogenic mutations are infrequent in oral squamous cell carcinoma of Asian origin.
title_short	Common oncogenic mutations are infrequent in oral squamous cell carcinoma of Asian origin.
title_full	Common oncogenic mutations are infrequent in oral squamous cell carcinoma of Asian origin.
title_fullStr	Common oncogenic mutations are infrequent in oral squamous cell carcinoma of Asian origin.
title_full_unstemmed	Common oncogenic mutations are infrequent in oral squamous cell carcinoma of Asian origin.
title_sort	common oncogenic mutations are infrequent in oral squamous cell carcinoma of asian origin.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/f2520dd8911c4275bef15354eea1df31
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Common oncogenic mutations are infrequent in oral squamous cell carcinoma of Asian origin.

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