Genotypic and Phenotypic Diversity of <named-content content-type="genus-species">Staphylococcus aureus</named-content> Isolates from Cystic Fibrosis Patient Lung Infections and Their Interactions with <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>

ABSTRACT Staphylococcus aureus has recently overtaken Pseudomonas aeruginosa as the most commonly recognized bacterial pathogen that infects the respiratory tracts of individuals with the genetic disease cystic fibrosis (CF) in the United States. Most studies of S. aureus in CF patient lung infectio...

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Autores principales:	Eryn E. Bernardy, Robert A. Petit, Vishnu Raghuram, Ashley M. Alexander, Timothy D. Read, Joanna B. Goldberg
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	Pseudomonas aeruginosa Staphylococcus aureus cystic fibrosis interspecies competition phylogenetic analysis Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/f25469ad4d33423088c203a751444efe
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spelling	oai:doaj.org-article:f25469ad4d33423088c203a751444efe2021-11-15T15:56:47ZGenotypic and Phenotypic Diversity of <named-content content-type="genus-species">Staphylococcus aureus</named-content> Isolates from Cystic Fibrosis Patient Lung Infections and Their Interactions with <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>10.1128/mBio.00735-202150-7511https://doaj.org/article/f25469ad4d33423088c203a751444efe2020-06-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00735-20https://doaj.org/toc/2150-7511ABSTRACT Staphylococcus aureus has recently overtaken Pseudomonas aeruginosa as the most commonly recognized bacterial pathogen that infects the respiratory tracts of individuals with the genetic disease cystic fibrosis (CF) in the United States. Most studies of S. aureus in CF patient lung infections have focused on a few isolates, often exclusively laboratory-adapted strains, and how they are killed by P. aeruginosa. Less is known about the diversity of S. aureus CF patient lung isolates in terms of both their virulence and their interaction with P. aeruginosa. To begin to address this gap, we recently sequenced 64 clinical S. aureus isolates and a reference isolate, JE2. Here, we analyzed the antibiotic resistance genotypes, sequence types, clonal complexes, spa types, agr types, and presence/absence of other known virulence factor genes of these isolates. We hypothesized that virulence phenotypes of S. aureus, namely, toxin production and the mucoid phenotype, would be lost in these isolates due to adaptation in the CF patient lung. In contrast to these expectations, we found that most isolates can lyse both rabbit and sheep blood (67.7%) and produce polysaccharide (69.2%), suggesting that these phenotypes were not lost during adaptation to the CF lung. We also identified three distinct phenotypic groups of S. aureus based on their survival in the presence of nonmucoid P. aeruginosa laboratory strain PAO1 and its mucoid derivative. Altogether, our work provides greater insight into the diversity of S. aureus isolates from CF patients, specifically the distribution of important virulence factors and their interaction with P. aeruginosa, all of which have implications in patient health. IMPORTANCE Staphylococcus aureus is now the most frequently detected recognized pathogen in the lungs of individuals who have cystic fibrosis (CF) in the United States, followed closely by Pseudomonas aeruginosa. When these pathogens are found to coinfect the CF lung, patients have a significantly worse prognosis. While P. aeruginosa has been rigorously studied in the context of bacterial pathogenesis in CF, less is known about S. aureus. Here, we present an in-depth study of 64 S. aureus clinical isolates from CF patients, for which we investigated genetic diversity utilizing whole-genome sequencing, virulence phenotypes, and interactions with P. aeruginosa. We found that S. aureus isolated from CF lungs are phylogenetically diverse; most retain known virulence factors and vary in their interactions with P. aeruginosa (i.e., they range from being highly sensitive to P. aeruginosa to completely tolerant to it). Deepening our understanding of how S. aureus responds to its environment and other microbes in the CF lung will enable future development of effective treatments and preventative measures against these formidable infections.Eryn E. BernardyRobert A. PetitVishnu RaghuramAshley M. AlexanderTimothy D. ReadJoanna B. GoldbergAmerican Society for MicrobiologyarticlePseudomonas aeruginosaStaphylococcus aureuscystic fibrosisinterspecies competitionphylogenetic analysisMicrobiologyQR1-502ENmBio, Vol 11, Iss 3 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pseudomonas aeruginosa Staphylococcus aureus cystic fibrosis interspecies competition phylogenetic analysis Microbiology QR1-502
spellingShingle	Pseudomonas aeruginosa Staphylococcus aureus cystic fibrosis interspecies competition phylogenetic analysis Microbiology QR1-502 Eryn E. Bernardy Robert A. Petit Vishnu Raghuram Ashley M. Alexander Timothy D. Read Joanna B. Goldberg Genotypic and Phenotypic Diversity of <named-content content-type="genus-species">Staphylococcus aureus</named-content> Isolates from Cystic Fibrosis Patient Lung Infections and Their Interactions with <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
description	ABSTRACT Staphylococcus aureus has recently overtaken Pseudomonas aeruginosa as the most commonly recognized bacterial pathogen that infects the respiratory tracts of individuals with the genetic disease cystic fibrosis (CF) in the United States. Most studies of S. aureus in CF patient lung infections have focused on a few isolates, often exclusively laboratory-adapted strains, and how they are killed by P. aeruginosa. Less is known about the diversity of S. aureus CF patient lung isolates in terms of both their virulence and their interaction with P. aeruginosa. To begin to address this gap, we recently sequenced 64 clinical S. aureus isolates and a reference isolate, JE2. Here, we analyzed the antibiotic resistance genotypes, sequence types, clonal complexes, spa types, agr types, and presence/absence of other known virulence factor genes of these isolates. We hypothesized that virulence phenotypes of S. aureus, namely, toxin production and the mucoid phenotype, would be lost in these isolates due to adaptation in the CF patient lung. In contrast to these expectations, we found that most isolates can lyse both rabbit and sheep blood (67.7%) and produce polysaccharide (69.2%), suggesting that these phenotypes were not lost during adaptation to the CF lung. We also identified three distinct phenotypic groups of S. aureus based on their survival in the presence of nonmucoid P. aeruginosa laboratory strain PAO1 and its mucoid derivative. Altogether, our work provides greater insight into the diversity of S. aureus isolates from CF patients, specifically the distribution of important virulence factors and their interaction with P. aeruginosa, all of which have implications in patient health. IMPORTANCE Staphylococcus aureus is now the most frequently detected recognized pathogen in the lungs of individuals who have cystic fibrosis (CF) in the United States, followed closely by Pseudomonas aeruginosa. When these pathogens are found to coinfect the CF lung, patients have a significantly worse prognosis. While P. aeruginosa has been rigorously studied in the context of bacterial pathogenesis in CF, less is known about S. aureus. Here, we present an in-depth study of 64 S. aureus clinical isolates from CF patients, for which we investigated genetic diversity utilizing whole-genome sequencing, virulence phenotypes, and interactions with P. aeruginosa. We found that S. aureus isolated from CF lungs are phylogenetically diverse; most retain known virulence factors and vary in their interactions with P. aeruginosa (i.e., they range from being highly sensitive to P. aeruginosa to completely tolerant to it). Deepening our understanding of how S. aureus responds to its environment and other microbes in the CF lung will enable future development of effective treatments and preventative measures against these formidable infections.
format	article
author	Eryn E. Bernardy Robert A. Petit Vishnu Raghuram Ashley M. Alexander Timothy D. Read Joanna B. Goldberg
author_facet	Eryn E. Bernardy Robert A. Petit Vishnu Raghuram Ashley M. Alexander Timothy D. Read Joanna B. Goldberg
author_sort	Eryn E. Bernardy
title	Genotypic and Phenotypic Diversity of <named-content content-type="genus-species">Staphylococcus aureus</named-content> Isolates from Cystic Fibrosis Patient Lung Infections and Their Interactions with <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
title_short	Genotypic and Phenotypic Diversity of <named-content content-type="genus-species">Staphylococcus aureus</named-content> Isolates from Cystic Fibrosis Patient Lung Infections and Their Interactions with <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
title_full	Genotypic and Phenotypic Diversity of <named-content content-type="genus-species">Staphylococcus aureus</named-content> Isolates from Cystic Fibrosis Patient Lung Infections and Their Interactions with <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
title_fullStr	Genotypic and Phenotypic Diversity of <named-content content-type="genus-species">Staphylococcus aureus</named-content> Isolates from Cystic Fibrosis Patient Lung Infections and Their Interactions with <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
title_full_unstemmed	Genotypic and Phenotypic Diversity of <named-content content-type="genus-species">Staphylococcus aureus</named-content> Isolates from Cystic Fibrosis Patient Lung Infections and Their Interactions with <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
title_sort	genotypic and phenotypic diversity of <named-content content-type="genus-species">staphylococcus aureus</named-content> isolates from cystic fibrosis patient lung infections and their interactions with <named-content content-type="genus-species">pseudomonas aeruginosa</named-content>
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/f25469ad4d33423088c203a751444efe
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Genotypic and Phenotypic Diversity of <named-content content-type="genus-species">Staphylococcus aureus</named-content> Isolates from Cystic Fibrosis Patient Lung Infections and Their Interactions with <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>

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