Inactivation efficacy of atmospheric air plasma and airborne acoustic ultrasound against bacterial biofilms

Abstract Biofilms are complex microbial communities that present serious contamination risks to our environment and health. In this study, atmospheric air plasma and airborne acoustic ultrasound technology were applied to inactivate Escherichia coli and Listeria innocua biofilms. Both technologies w...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Apurva D. Patange, Jeremy C. Simpson, James F. Curtin, Catherine M. Burgess, P. J. Cullen, Brijesh K. Tiwari
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f254772cb4d14fde8f0f557f977d2873
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f254772cb4d14fde8f0f557f977d2873
record_format	dspace
spelling	oai:doaj.org-article:f254772cb4d14fde8f0f557f977d28732021-12-02T13:23:50ZInactivation efficacy of atmospheric air plasma and airborne acoustic ultrasound against bacterial biofilms10.1038/s41598-021-81977-z2045-2322https://doaj.org/article/f254772cb4d14fde8f0f557f977d28732021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81977-zhttps://doaj.org/toc/2045-2322Abstract Biofilms are complex microbial communities that present serious contamination risks to our environment and health. In this study, atmospheric air plasma and airborne acoustic ultrasound technology were applied to inactivate Escherichia coli and Listeria innocua biofilms. Both technologies were efficient in controlling, or completely inactivating, the target bacterial biofilms. Viability and metabolic assays, along with microscopy analysis, revealed that atmospheric air plasma and airborne acoustic ultrasound damaged both the bacterial biofilm cells and its structural integrity. Scanning electron microscopy images highlighted the disruption of the biofilms and pore formation in bacterial cells exposed to both the plasma and acoustic treatments. Elevated reactive oxygen and nitrogen species in bacterial cells treated with atmospheric air plasma, demonstrated their primary role in the observed bacterial inactivation process. Our findings provide potential antimicrobial strategies to combat bacterial biofilms in the food and healthcare sectors.Apurva D. PatangeJeremy C. SimpsonJames F. CurtinCatherine M. BurgessP. J. CullenBrijesh K. TiwariNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Apurva D. Patange Jeremy C. Simpson James F. Curtin Catherine M. Burgess P. J. Cullen Brijesh K. Tiwari Inactivation efficacy of atmospheric air plasma and airborne acoustic ultrasound against bacterial biofilms
description	Abstract Biofilms are complex microbial communities that present serious contamination risks to our environment and health. In this study, atmospheric air plasma and airborne acoustic ultrasound technology were applied to inactivate Escherichia coli and Listeria innocua biofilms. Both technologies were efficient in controlling, or completely inactivating, the target bacterial biofilms. Viability and metabolic assays, along with microscopy analysis, revealed that atmospheric air plasma and airborne acoustic ultrasound damaged both the bacterial biofilm cells and its structural integrity. Scanning electron microscopy images highlighted the disruption of the biofilms and pore formation in bacterial cells exposed to both the plasma and acoustic treatments. Elevated reactive oxygen and nitrogen species in bacterial cells treated with atmospheric air plasma, demonstrated their primary role in the observed bacterial inactivation process. Our findings provide potential antimicrobial strategies to combat bacterial biofilms in the food and healthcare sectors.
format	article
author	Apurva D. Patange Jeremy C. Simpson James F. Curtin Catherine M. Burgess P. J. Cullen Brijesh K. Tiwari
author_facet	Apurva D. Patange Jeremy C. Simpson James F. Curtin Catherine M. Burgess P. J. Cullen Brijesh K. Tiwari
author_sort	Apurva D. Patange
title	Inactivation efficacy of atmospheric air plasma and airborne acoustic ultrasound against bacterial biofilms
title_short	Inactivation efficacy of atmospheric air plasma and airborne acoustic ultrasound against bacterial biofilms
title_full	Inactivation efficacy of atmospheric air plasma and airborne acoustic ultrasound against bacterial biofilms
title_fullStr	Inactivation efficacy of atmospheric air plasma and airborne acoustic ultrasound against bacterial biofilms
title_full_unstemmed	Inactivation efficacy of atmospheric air plasma and airborne acoustic ultrasound against bacterial biofilms
title_sort	inactivation efficacy of atmospheric air plasma and airborne acoustic ultrasound against bacterial biofilms
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f254772cb4d14fde8f0f557f977d2873
work_keys_str_mv	AT apurvadpatange inactivationefficacyofatmosphericairplasmaandairborneacousticultrasoundagainstbacterialbiofilms AT jeremycsimpson inactivationefficacyofatmosphericairplasmaandairborneacousticultrasoundagainstbacterialbiofilms AT jamesfcurtin inactivationefficacyofatmosphericairplasmaandairborneacousticultrasoundagainstbacterialbiofilms AT catherinemburgess inactivationefficacyofatmosphericairplasmaandairborneacousticultrasoundagainstbacterialbiofilms AT pjcullen inactivationefficacyofatmosphericairplasmaandairborneacousticultrasoundagainstbacterialbiofilms AT brijeshktiwari inactivationefficacyofatmosphericairplasmaandairborneacousticultrasoundagainstbacterialbiofilms
_version_	1718393188129964032

Inactivation efficacy of atmospheric air plasma and airborne acoustic ultrasound against bacterial biofilms

Ejemplares similares