Free radical scavenging in vitro and biological activity of diphenyl diselenide-loaded nanocapsules: DPDS-NCS antioxidant and toxicological effects

Sílvio Terra Stefanello,1 Fernando Dobrachinski,1 Nélson Rodrigues de Carvalho,1 Guilherme Pires Amaral,1 Rômulo Pillon Barcelos,1 Vitor Antunes Oliveira,1 Cláudia Sirlene Oliveira,1 Camila Ferrazza Alves Giordani,2 Maria Ester Pereira,1 Oscar Endrigo Dorne...

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Autores principales:	Stefanello ST, Dobrachinski F, Carvalho NR, Amaral GP, Barcelos RP, Oliveira VA, Oliveira CS, Giordani CFA, Pereira ME, Rodrigues OED, Soares FAA
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/f254f747fdd344afa66e8dcfbfc87214
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spelling	oai:doaj.org-article:f254f747fdd344afa66e8dcfbfc872142021-12-02T05:57:51ZFree radical scavenging in vitro and biological activity of diphenyl diselenide-loaded nanocapsules: DPDS-NCS antioxidant and toxicological effects1178-2013https://doaj.org/article/f254f747fdd344afa66e8dcfbfc872142015-09-01T00:00:00Zhttps://www.dovepress.com/free-radical-scavenging-in-vitro-and-biological-activity-of-diphenyl-d-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Sílvio Terra Stefanello,1 Fernando Dobrachinski,1 Nélson Rodrigues de Carvalho,1 Guilherme Pires Amaral,1 Rômulo Pillon Barcelos,1 Vitor Antunes Oliveira,1 Cláudia Sirlene Oliveira,1 Camila Ferrazza Alves Giordani,2 Maria Ester Pereira,1 Oscar Endrigo Dorneles Rodrigues,2 Félix Alexandre Antunes Soares1 1Departamento de Bioquímica e Biologia Molecular, 2Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, Brazil Abstract: Selenium compounds, such as diphenyl diselenide (DPDS), have been shown to exhibit biological activity, including antioxidant effects. However, the use of DPDS in pharmacology is limited due to in vivo pro-oxidative effects. In addition, studies have shown that DPDS-loaded nanocapsules (DPDS-NCS) have greater bioavailability than free DPDS in mice. Accordingly, the aim of this study was to investigate the antioxidant properties of DPDS-NCS in vitro and biological activity in mice. Our in vitro results suggested that DPDS-NCS significantly reduced the production of reactive oxygen species and Fe(II)-induced lipid peroxidation (LPO) in brain. The administration of DPDS-NCS did not result in death or change the levels of endogenous reduced or oxidized glutathione after 72 hours of exposure. Moreover, ex vivo assays demonstrated that DPDS-NCS significantly decreased the LPO and reactive oxygen species levels in the brain. In addition, the highest dose of DPDS-NCS significantly reduced Fe(II)- and sodium nitroprusside-induced LPO in the brain and Fe(II)-induced LPO in the liver. Also, δ-aminolevulinate acid dehydratase within the brain was inhibited only in the highest dose of DPDS-NCS. In conclusion, our data demonstrated that DPDS-NCS exhibited low toxicity in mice and have significant antioxidant characteristics, indicating that nanoencapsulation is a safer method of DPDS administration. Keywords: diphenyl diselenide, DPDS-NCS, lipid peroxidation, antioxidant properties, δ-ALA-DStefanello STDobrachinski FCarvalho NRAmaral GPBarcelos RPOliveira VAOliveira CSGiordani CFAPereira MERodrigues OEDSoares FAADove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 5663-5670 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Stefanello ST Dobrachinski F Carvalho NR Amaral GP Barcelos RP Oliveira VA Oliveira CS Giordani CFA Pereira ME Rodrigues OED Soares FAA Free radical scavenging in vitro and biological activity of diphenyl diselenide-loaded nanocapsules: DPDS-NCS antioxidant and toxicological effects
description	Sílvio Terra Stefanello,1 Fernando Dobrachinski,1 Nélson Rodrigues de Carvalho,1 Guilherme Pires Amaral,1 Rômulo Pillon Barcelos,1 Vitor Antunes Oliveira,1 Cláudia Sirlene Oliveira,1 Camila Ferrazza Alves Giordani,2 Maria Ester Pereira,1 Oscar Endrigo Dorneles Rodrigues,2 Félix Alexandre Antunes Soares1 1Departamento de Bioquímica e Biologia Molecular, 2Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, Brazil Abstract: Selenium compounds, such as diphenyl diselenide (DPDS), have been shown to exhibit biological activity, including antioxidant effects. However, the use of DPDS in pharmacology is limited due to in vivo pro-oxidative effects. In addition, studies have shown that DPDS-loaded nanocapsules (DPDS-NCS) have greater bioavailability than free DPDS in mice. Accordingly, the aim of this study was to investigate the antioxidant properties of DPDS-NCS in vitro and biological activity in mice. Our in vitro results suggested that DPDS-NCS significantly reduced the production of reactive oxygen species and Fe(II)-induced lipid peroxidation (LPO) in brain. The administration of DPDS-NCS did not result in death or change the levels of endogenous reduced or oxidized glutathione after 72 hours of exposure. Moreover, ex vivo assays demonstrated that DPDS-NCS significantly decreased the LPO and reactive oxygen species levels in the brain. In addition, the highest dose of DPDS-NCS significantly reduced Fe(II)- and sodium nitroprusside-induced LPO in the brain and Fe(II)-induced LPO in the liver. Also, δ-aminolevulinate acid dehydratase within the brain was inhibited only in the highest dose of DPDS-NCS. In conclusion, our data demonstrated that DPDS-NCS exhibited low toxicity in mice and have significant antioxidant characteristics, indicating that nanoencapsulation is a safer method of DPDS administration. Keywords: diphenyl diselenide, DPDS-NCS, lipid peroxidation, antioxidant properties, δ-ALA-D
format	article
author	Stefanello ST Dobrachinski F Carvalho NR Amaral GP Barcelos RP Oliveira VA Oliveira CS Giordani CFA Pereira ME Rodrigues OED Soares FAA
author_facet	Stefanello ST Dobrachinski F Carvalho NR Amaral GP Barcelos RP Oliveira VA Oliveira CS Giordani CFA Pereira ME Rodrigues OED Soares FAA
author_sort	Stefanello ST
title	Free radical scavenging in vitro and biological activity of diphenyl diselenide-loaded nanocapsules: DPDS-NCS antioxidant and toxicological effects
title_short	Free radical scavenging in vitro and biological activity of diphenyl diselenide-loaded nanocapsules: DPDS-NCS antioxidant and toxicological effects
title_full	Free radical scavenging in vitro and biological activity of diphenyl diselenide-loaded nanocapsules: DPDS-NCS antioxidant and toxicological effects
title_fullStr	Free radical scavenging in vitro and biological activity of diphenyl diselenide-loaded nanocapsules: DPDS-NCS antioxidant and toxicological effects
title_full_unstemmed	Free radical scavenging in vitro and biological activity of diphenyl diselenide-loaded nanocapsules: DPDS-NCS antioxidant and toxicological effects
title_sort	free radical scavenging in vitro and biological activity of diphenyl diselenide-loaded nanocapsules: dpds-ncs antioxidant and toxicological effects
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/f254f747fdd344afa66e8dcfbfc87214
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Free radical scavenging in vitro and biological activity of diphenyl diselenide-loaded nanocapsules: DPDS-NCS antioxidant and toxicological effects

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