Peripheral Neuropathy Presents Similar Symptoms and Pathological Changes in Both High-Fat Diet and Pharmacologically Induced Pre- and Diabetic Mouse Models

The objective of the study was to compare the effects of experimentally induced type 1 or type 2 diabetes (T1D or T2D) on the functional, structural and biochemical properties of mouse peripheral nerves. Eight-week-old C57BL/6 mice were randomly assigned into three groups, including the control (CTR...

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Autores principales:	Julia Jaroslawska, Agnieszka Korytko, Kamila Zglejc-Waszak, Tomasz Antonowski, Andrzej S. Pomianowski, Krzysztof Wasowicz, Joanna Wojtkiewicz, Judyta K. Juranek
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	diabetic peripheral neuropathy sciatic nerve diabetic mouse models streptozotocin high-fat diet Science Q
Acceso en línea:	https://doaj.org/article/f256b20a853941649fc3d8db76f58359
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spelling	oai:doaj.org-article:f256b20a853941649fc3d8db76f583592021-11-25T18:11:42ZPeripheral Neuropathy Presents Similar Symptoms and Pathological Changes in Both High-Fat Diet and Pharmacologically Induced Pre- and Diabetic Mouse Models10.3390/life111112672075-1729https://doaj.org/article/f256b20a853941649fc3d8db76f583592021-11-01T00:00:00Zhttps://www.mdpi.com/2075-1729/11/11/1267https://doaj.org/toc/2075-1729The objective of the study was to compare the effects of experimentally induced type 1 or type 2 diabetes (T1D or T2D) on the functional, structural and biochemical properties of mouse peripheral nerves. Eight-week-old C57BL/6 mice were randomly assigned into three groups, including the control (CTRL, chow-fed), STZ (streptozotocin (STZ)-injected), and HFD (high-fat diet (HFD)-fed) group. After 18-weeks of experimental treatment, HFD mice had higher body weights and elevated levels of plasma lipids, while STZ mice developed hyperglycemia. STZ-treated mice, after an extended period of untreated diabetes, developed motor and sensory nerve conduction-velocity deficits. Moreover, relative to control fibers, pre- and diabetic axons were lower in number and irregular in shape. Animals from both treatment groups manifested a pronounced overexpression of nNOS and a reduced expression of SOD1 proteins in the sciatic nerve, indicating oxidative–nitrosative stress and ineffective antioxidant protection in the peripheral nervous system of these mice. Collectively, STZ- and HFD-treated mice revealed similar characteristics of peripheral nerve damage, including a number of morphological and electrophysiological pathologies in the sciatic nerve. While hyperglycemia is a large component of diabetic neuropathy pathogenesis, the non-hyperglycemic effects of diabetes, including dyslipidemia, may also be of importance in the development of this condition.Julia JaroslawskaAgnieszka KorytkoKamila Zglejc-WaszakTomasz AntonowskiAndrzej S. PomianowskiKrzysztof WasowiczJoanna WojtkiewiczJudyta K. JuranekMDPI AGarticlediabetic peripheral neuropathysciatic nervediabetic mouse modelsstreptozotocinhigh-fat dietScienceQENLife, Vol 11, Iss 1267, p 1267 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	diabetic peripheral neuropathy sciatic nerve diabetic mouse models streptozotocin high-fat diet Science Q
spellingShingle	diabetic peripheral neuropathy sciatic nerve diabetic mouse models streptozotocin high-fat diet Science Q Julia Jaroslawska Agnieszka Korytko Kamila Zglejc-Waszak Tomasz Antonowski Andrzej S. Pomianowski Krzysztof Wasowicz Joanna Wojtkiewicz Judyta K. Juranek Peripheral Neuropathy Presents Similar Symptoms and Pathological Changes in Both High-Fat Diet and Pharmacologically Induced Pre- and Diabetic Mouse Models
description	The objective of the study was to compare the effects of experimentally induced type 1 or type 2 diabetes (T1D or T2D) on the functional, structural and biochemical properties of mouse peripheral nerves. Eight-week-old C57BL/6 mice were randomly assigned into three groups, including the control (CTRL, chow-fed), STZ (streptozotocin (STZ)-injected), and HFD (high-fat diet (HFD)-fed) group. After 18-weeks of experimental treatment, HFD mice had higher body weights and elevated levels of plasma lipids, while STZ mice developed hyperglycemia. STZ-treated mice, after an extended period of untreated diabetes, developed motor and sensory nerve conduction-velocity deficits. Moreover, relative to control fibers, pre- and diabetic axons were lower in number and irregular in shape. Animals from both treatment groups manifested a pronounced overexpression of nNOS and a reduced expression of SOD1 proteins in the sciatic nerve, indicating oxidative–nitrosative stress and ineffective antioxidant protection in the peripheral nervous system of these mice. Collectively, STZ- and HFD-treated mice revealed similar characteristics of peripheral nerve damage, including a number of morphological and electrophysiological pathologies in the sciatic nerve. While hyperglycemia is a large component of diabetic neuropathy pathogenesis, the non-hyperglycemic effects of diabetes, including dyslipidemia, may also be of importance in the development of this condition.
format	article
author	Julia Jaroslawska Agnieszka Korytko Kamila Zglejc-Waszak Tomasz Antonowski Andrzej S. Pomianowski Krzysztof Wasowicz Joanna Wojtkiewicz Judyta K. Juranek
author_facet	Julia Jaroslawska Agnieszka Korytko Kamila Zglejc-Waszak Tomasz Antonowski Andrzej S. Pomianowski Krzysztof Wasowicz Joanna Wojtkiewicz Judyta K. Juranek
author_sort	Julia Jaroslawska
title	Peripheral Neuropathy Presents Similar Symptoms and Pathological Changes in Both High-Fat Diet and Pharmacologically Induced Pre- and Diabetic Mouse Models
title_short	Peripheral Neuropathy Presents Similar Symptoms and Pathological Changes in Both High-Fat Diet and Pharmacologically Induced Pre- and Diabetic Mouse Models
title_full	Peripheral Neuropathy Presents Similar Symptoms and Pathological Changes in Both High-Fat Diet and Pharmacologically Induced Pre- and Diabetic Mouse Models
title_fullStr	Peripheral Neuropathy Presents Similar Symptoms and Pathological Changes in Both High-Fat Diet and Pharmacologically Induced Pre- and Diabetic Mouse Models
title_full_unstemmed	Peripheral Neuropathy Presents Similar Symptoms and Pathological Changes in Both High-Fat Diet and Pharmacologically Induced Pre- and Diabetic Mouse Models
title_sort	peripheral neuropathy presents similar symptoms and pathological changes in both high-fat diet and pharmacologically induced pre- and diabetic mouse models
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f256b20a853941649fc3d8db76f58359
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Peripheral Neuropathy Presents Similar Symptoms and Pathological Changes in Both High-Fat Diet and Pharmacologically Induced Pre- and Diabetic Mouse Models

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