Electroacupuncture Ameliorates Depressive-Like Behaviors in Poststroke Rats via Activating the tPA/BDNF/TrkB Pathway

Hao Dong,1,* Yan-Qiang Qin,2,* Ying-Chun Sun,2,* Hai-Jiang Yao,2 Xian-Kuan Cheng,2 Yan Yu,1 Shou-Si Lu1 1Beijing Bo’ai Hospital, China Rehabilitation Research Center, China Rehabilitation Science Institute, Beijing, People’s Republic of China; 2Treatment Center of TCM, Beijing Bo...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Dong H, Qin YQ, Sun YC, Yao HJ, Cheng XK, Yu Y, Lu SS
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	electroacupuncture poststroke depression depressive-like behaviors tpa/bdnf/trkb chronic unpredictable mild stimulation Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/f2573050a82645339d34bcc4e4f94701
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f2573050a82645339d34bcc4e4f94701
record_format	dspace
spelling	oai:doaj.org-article:f2573050a82645339d34bcc4e4f947012021-12-02T14:21:25ZElectroacupuncture Ameliorates Depressive-Like Behaviors in Poststroke Rats via Activating the tPA/BDNF/TrkB Pathway1178-2021https://doaj.org/article/f2573050a82645339d34bcc4e4f947012021-04-01T00:00:00Zhttps://www.dovepress.com/electroacupuncture-ameliorates-depressive-like-behaviors-in-poststroke-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Hao Dong,1,* Yan-Qiang Qin,2,* Ying-Chun Sun,2,* Hai-Jiang Yao,2 Xian-Kuan Cheng,2 Yan Yu,1 Shou-Si Lu1 1Beijing Bo’ai Hospital, China Rehabilitation Research Center, China Rehabilitation Science Institute, Beijing, People’s Republic of China; 2Treatment Center of TCM, Beijing Bo’ai Hospital, China Rehabilitation Research Center, China Rehabilitation Science Institute, School of Rehabilitation Medicine, Capital Medical University, Beijing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yan-Qiang Qin; Ying-Chun SunTreatment Center of TCM, Beijing Bo’ai Hospital, China Rehabilitation Research Center, China Rehabilitation Science Institute, No. 10 Jiaomen North Road, Beijing, 100068, People’s Republic of ChinaTel +86 18612251241Email yanqiangqin@126.com; syccrrc@sina.comBackground: Electroacupuncture (EA) is a form of physical therapy that has been widely used in clinical practice in China. Post-stroke depression (PSD) is the most common neuropsychiatric complication after stroke. EA has been shown to have beneficial effects on PSD patients. However, the potential mechanism underlying the protective effects of EA on PSD remains unclear. Here, we investigated whether tissue plasminogen activator (tPA)/brain-derived neurotrophic factor (BDNF)/tyrosine kinase receptor B (TrkB) signaling pathway participates in the therapeutic effects of EA in a rat PSD model.Methods: Experimental PSD was induced by combining middle cerebral artery occlusion (MCAO) with chronic unpredictable mild stimulation (CUMS) in adult male rats. Bodyweight gain, neurological score, sucrose preference, and open field test were determined at 0, 7, 14, and 35 days after completing MCAO. The protein expressions of tPA, precursor BDNF (proBDNF), mature BDNF (mBDNF), and TrkB were measured by immunofluorescence and Western blot analysis. The tPA inhibitor plasminogen inhibitor-1 (PAI-1) was used to explore whether tPA plays a crucial role in the protective effects of EA on PSD.Results: Compared with the sham rats, the PSD rats showed decreased bodyweight, deteriorated neurological score, and significant depressive-like behaviors. EA remarkably reversed bodyweight loss, neurological deficit, and depressive-like behaviors in PSD rats. Immunofluorescence staining and Western blot analysis showed that PSD-induced decreased expression of tPA, mBDNF, and TrkB were prevented by EA. Furthermore, we found that the effects of EA against PSD-induced depressive-like behaviors were abolished by PAI-1, the specific inhibitor of tPA.Conclusion: Our results suggest that the improvement in depressive-like behaviors induced by EA is likely achieved via activation of the tPA/BDNF/TrkB pathway.Keywords: electroacupuncture, poststroke depression, depressive-like behaviors, tPA/BDNF/TrkB, chronic unpredictable mild stimulationDong HQin YQSun YCYao HJCheng XKYu YLu SSDove Medical Pressarticleelectroacupuncturepoststroke depressiondepressive-like behaviorstpa/bdnf/trkbchronic unpredictable mild stimulationNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 17, Pp 1057-1067 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	electroacupuncture poststroke depression depressive-like behaviors tpa/bdnf/trkb chronic unpredictable mild stimulation Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	electroacupuncture poststroke depression depressive-like behaviors tpa/bdnf/trkb chronic unpredictable mild stimulation Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Dong H Qin YQ Sun YC Yao HJ Cheng XK Yu Y Lu SS Electroacupuncture Ameliorates Depressive-Like Behaviors in Poststroke Rats via Activating the tPA/BDNF/TrkB Pathway
description	Hao Dong,1,* Yan-Qiang Qin,2,* Ying-Chun Sun,2,* Hai-Jiang Yao,2 Xian-Kuan Cheng,2 Yan Yu,1 Shou-Si Lu1 1Beijing Bo’ai Hospital, China Rehabilitation Research Center, China Rehabilitation Science Institute, Beijing, People’s Republic of China; 2Treatment Center of TCM, Beijing Bo’ai Hospital, China Rehabilitation Research Center, China Rehabilitation Science Institute, School of Rehabilitation Medicine, Capital Medical University, Beijing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yan-Qiang Qin; Ying-Chun SunTreatment Center of TCM, Beijing Bo’ai Hospital, China Rehabilitation Research Center, China Rehabilitation Science Institute, No. 10 Jiaomen North Road, Beijing, 100068, People’s Republic of ChinaTel +86 18612251241Email yanqiangqin@126.com; syccrrc@sina.comBackground: Electroacupuncture (EA) is a form of physical therapy that has been widely used in clinical practice in China. Post-stroke depression (PSD) is the most common neuropsychiatric complication after stroke. EA has been shown to have beneficial effects on PSD patients. However, the potential mechanism underlying the protective effects of EA on PSD remains unclear. Here, we investigated whether tissue plasminogen activator (tPA)/brain-derived neurotrophic factor (BDNF)/tyrosine kinase receptor B (TrkB) signaling pathway participates in the therapeutic effects of EA in a rat PSD model.Methods: Experimental PSD was induced by combining middle cerebral artery occlusion (MCAO) with chronic unpredictable mild stimulation (CUMS) in adult male rats. Bodyweight gain, neurological score, sucrose preference, and open field test were determined at 0, 7, 14, and 35 days after completing MCAO. The protein expressions of tPA, precursor BDNF (proBDNF), mature BDNF (mBDNF), and TrkB were measured by immunofluorescence and Western blot analysis. The tPA inhibitor plasminogen inhibitor-1 (PAI-1) was used to explore whether tPA plays a crucial role in the protective effects of EA on PSD.Results: Compared with the sham rats, the PSD rats showed decreased bodyweight, deteriorated neurological score, and significant depressive-like behaviors. EA remarkably reversed bodyweight loss, neurological deficit, and depressive-like behaviors in PSD rats. Immunofluorescence staining and Western blot analysis showed that PSD-induced decreased expression of tPA, mBDNF, and TrkB were prevented by EA. Furthermore, we found that the effects of EA against PSD-induced depressive-like behaviors were abolished by PAI-1, the specific inhibitor of tPA.Conclusion: Our results suggest that the improvement in depressive-like behaviors induced by EA is likely achieved via activation of the tPA/BDNF/TrkB pathway.Keywords: electroacupuncture, poststroke depression, depressive-like behaviors, tPA/BDNF/TrkB, chronic unpredictable mild stimulation
format	article
author	Dong H Qin YQ Sun YC Yao HJ Cheng XK Yu Y Lu SS
author_facet	Dong H Qin YQ Sun YC Yao HJ Cheng XK Yu Y Lu SS
author_sort	Dong H
title	Electroacupuncture Ameliorates Depressive-Like Behaviors in Poststroke Rats via Activating the tPA/BDNF/TrkB Pathway
title_short	Electroacupuncture Ameliorates Depressive-Like Behaviors in Poststroke Rats via Activating the tPA/BDNF/TrkB Pathway
title_full	Electroacupuncture Ameliorates Depressive-Like Behaviors in Poststroke Rats via Activating the tPA/BDNF/TrkB Pathway
title_fullStr	Electroacupuncture Ameliorates Depressive-Like Behaviors in Poststroke Rats via Activating the tPA/BDNF/TrkB Pathway
title_full_unstemmed	Electroacupuncture Ameliorates Depressive-Like Behaviors in Poststroke Rats via Activating the tPA/BDNF/TrkB Pathway
title_sort	electroacupuncture ameliorates depressive-like behaviors in poststroke rats via activating the tpa/bdnf/trkb pathway
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/f2573050a82645339d34bcc4e4f94701
work_keys_str_mv	AT dongh electroacupunctureamelioratesdepressivelikebehaviorsinpoststrokeratsviaactivatingthetpabdnftrkbpathway AT qinyq electroacupunctureamelioratesdepressivelikebehaviorsinpoststrokeratsviaactivatingthetpabdnftrkbpathway AT sunyc electroacupunctureamelioratesdepressivelikebehaviorsinpoststrokeratsviaactivatingthetpabdnftrkbpathway AT yaohj electroacupunctureamelioratesdepressivelikebehaviorsinpoststrokeratsviaactivatingthetpabdnftrkbpathway AT chengxk electroacupunctureamelioratesdepressivelikebehaviorsinpoststrokeratsviaactivatingthetpabdnftrkbpathway AT yuy electroacupunctureamelioratesdepressivelikebehaviorsinpoststrokeratsviaactivatingthetpabdnftrkbpathway AT luss electroacupunctureamelioratesdepressivelikebehaviorsinpoststrokeratsviaactivatingthetpabdnftrkbpathway
_version_	1718391582535712768

Electroacupuncture Ameliorates Depressive-Like Behaviors in Poststroke Rats via Activating the tPA/BDNF/TrkB Pathway

Ejemplares similares