The role of melanocortin 1 receptor on melanogenesis pathway in skin color differentiation of red tilapia

The skin color differentiation and variation are the main problems limiting the development of red tilapia commercial culture. The specific function and regulation mechanism of mc1r gene in fish pigmentation are still poorly understood. In this study, we investigated the mc1r sequence characteristic...

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Autores principales:	Lan-mei Wang, Bing-jie Jiang, Wen-bin Zhu, Jian-jun Fu, Ming-kun Luo, Wei Liu, Zai-jie Dong
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Red tilapia Melanocortin 1 recepto (mc1r) Skin color differentiation and variation Gene expression Localization ɑ-MSH Aquaculture. Fisheries. Angling SH1-691
Acceso en línea:	https://doaj.org/article/f25ea3d78d70441b9c8f47178b1f1e46
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spelling	oai:doaj.org-article:f25ea3d78d70441b9c8f47178b1f1e462021-11-20T05:07:19ZThe role of melanocortin 1 receptor on melanogenesis pathway in skin color differentiation of red tilapia2352-513410.1016/j.aqrep.2021.100946https://doaj.org/article/f25ea3d78d70441b9c8f47178b1f1e462022-02-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S235251342100363Xhttps://doaj.org/toc/2352-5134The skin color differentiation and variation are the main problems limiting the development of red tilapia commercial culture. The specific function and regulation mechanism of mc1r gene in fish pigmentation are still poorly understood. In this study, we investigated the mc1r sequence characteristics, expression profiles, localization and melanocortins (MSH)-mc1r role on melanogenesis pathway of red tilapia. The mc1r ORF of red tilapia is1056 bp, corresponding to 351 amino acid, which belongs to a family of G-protein-coupled receptors. Quantitative real-time PCR demonstrated that mc1r mRNA was mainly expressed in the brain and dorsal skin tissues of PB (pink with scattered black spots) fish. The mc1r gene was found to be expressed during early development stages of red tilapia. The mc1r mRNA expressions were high in the 48 h post-hatching (48hph), 24hph and cleavage stages. Immunofluorescence analysis revealed that Mc1r was concentrated mainly in the cytoplasm and intercellular substance of skin epidermis. Injection with caudal vein of ɑ-MSH at 2.5 mg g−1 resulted in significantly higher tyrosinase activity and melanin level in the dorsal and ventral skin of red tilapia. The expression of mc1r and tyr mRNA in the skin of red tilapia increased after ɑ-MSH injection. These results suggested that mc1r plays an important role in fish melanogenesis pathway through binding to α-MSH.Lan-mei WangBing-jie JiangWen-bin ZhuJian-jun FuMing-kun LuoWei LiuZai-jie DongElsevierarticleRed tilapiaMelanocortin 1 recepto (mc1r)Skin color differentiation and variationGene expressionLocalizationɑ-MSHAquaculture. Fisheries. AnglingSH1-691ENAquaculture Reports, Vol 22, Iss , Pp 100946- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Red tilapia Melanocortin 1 recepto (mc1r) Skin color differentiation and variation Gene expression Localization ɑ-MSH Aquaculture. Fisheries. Angling SH1-691
spellingShingle	Red tilapia Melanocortin 1 recepto (mc1r) Skin color differentiation and variation Gene expression Localization ɑ-MSH Aquaculture. Fisheries. Angling SH1-691 Lan-mei Wang Bing-jie Jiang Wen-bin Zhu Jian-jun Fu Ming-kun Luo Wei Liu Zai-jie Dong The role of melanocortin 1 receptor on melanogenesis pathway in skin color differentiation of red tilapia
description	The skin color differentiation and variation are the main problems limiting the development of red tilapia commercial culture. The specific function and regulation mechanism of mc1r gene in fish pigmentation are still poorly understood. In this study, we investigated the mc1r sequence characteristics, expression profiles, localization and melanocortins (MSH)-mc1r role on melanogenesis pathway of red tilapia. The mc1r ORF of red tilapia is1056 bp, corresponding to 351 amino acid, which belongs to a family of G-protein-coupled receptors. Quantitative real-time PCR demonstrated that mc1r mRNA was mainly expressed in the brain and dorsal skin tissues of PB (pink with scattered black spots) fish. The mc1r gene was found to be expressed during early development stages of red tilapia. The mc1r mRNA expressions were high in the 48 h post-hatching (48hph), 24hph and cleavage stages. Immunofluorescence analysis revealed that Mc1r was concentrated mainly in the cytoplasm and intercellular substance of skin epidermis. Injection with caudal vein of ɑ-MSH at 2.5 mg g−1 resulted in significantly higher tyrosinase activity and melanin level in the dorsal and ventral skin of red tilapia. The expression of mc1r and tyr mRNA in the skin of red tilapia increased after ɑ-MSH injection. These results suggested that mc1r plays an important role in fish melanogenesis pathway through binding to α-MSH.
format	article
author	Lan-mei Wang Bing-jie Jiang Wen-bin Zhu Jian-jun Fu Ming-kun Luo Wei Liu Zai-jie Dong
author_facet	Lan-mei Wang Bing-jie Jiang Wen-bin Zhu Jian-jun Fu Ming-kun Luo Wei Liu Zai-jie Dong
author_sort	Lan-mei Wang
title	The role of melanocortin 1 receptor on melanogenesis pathway in skin color differentiation of red tilapia
title_short	The role of melanocortin 1 receptor on melanogenesis pathway in skin color differentiation of red tilapia
title_full	The role of melanocortin 1 receptor on melanogenesis pathway in skin color differentiation of red tilapia
title_fullStr	The role of melanocortin 1 receptor on melanogenesis pathway in skin color differentiation of red tilapia
title_full_unstemmed	The role of melanocortin 1 receptor on melanogenesis pathway in skin color differentiation of red tilapia
title_sort	role of melanocortin 1 receptor on melanogenesis pathway in skin color differentiation of red tilapia
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/f25ea3d78d70441b9c8f47178b1f1e46
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The role of melanocortin 1 receptor on melanogenesis pathway in skin color differentiation of red tilapia

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