Analysis of the relationship between bile duct and duodenal microbiota reveals that potential dysbacteriosis is the main cause of primary common bile duct stones

Bacteria play an important role in the formation of primary Common Bile Duct (CBD) stones. However, the composition and function of the microbiota of bile duct in patients with primary CBD stones remained to be explored. We utilized the 16S rRNA gene high-throughput sequencing technology to analyze...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhitang Lyu, Tingting Yu, Lichao Zhang, Xiaona Xu, Yijun Zhang, Jihong Li, Zhirong Li, Wei Zhang, Senlin Hou
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2021
Materias:	Primary common bile duct stones ERCP Dysbacteriosis Bile duct microbiota Duodenal microbiota 16S rRNA gene high-throughput sequencing Biotechnology TP248.13-248.65 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f26084fc5ae74b7fbee5bf7279d275eb
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f26084fc5ae74b7fbee5bf7279d275eb
record_format	dspace
spelling	oai:doaj.org-article:f26084fc5ae74b7fbee5bf7279d275eb2021-11-26T04:35:34ZAnalysis of the relationship between bile duct and duodenal microbiota reveals that potential dysbacteriosis is the main cause of primary common bile duct stones2405-805X10.1016/j.synbio.2021.11.002https://doaj.org/article/f26084fc5ae74b7fbee5bf7279d275eb2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2405805X21000703https://doaj.org/toc/2405-805XBacteria play an important role in the formation of primary Common Bile Duct (CBD) stones. However, the composition and function of the microbiota of bile duct in patients with primary CBD stones remained to be explored. We utilized the 16S rRNA gene high-throughput sequencing technology to analyze the microbial diversity and community composition of biliary and duodenal microbiota in 15 patients with primary CBD stones and 4 patients without biliary tract diseases. Alpha diversity analysis showed that the microbiota richness was similar in bile and intestinal fluid; Beta diversity analysis showed that there were differences in the composition between biliary microbiota and the duodenal microbiota, but the abundance of the main groups showed similarities. The composition of the biliary microbiota from gallstone patients was more complex, as was the duodenal microbiota. Proteobacteria and Firmicutes were the dominant bacteria at phylum level, accounting for at least 75% of the total reads in each subgroup. Pseudomonas and Escherichia−Shigella were the major genus among subgroups, but Escherichia-Shigella had increased abundance in duodenal microbiota with primary choledocholithiasis, which may play an important role in stone formation. It is noteworthy that Clostridiumsensu_stricto, Lachnospiraceae _UCG-008, Butyrivibrio and Roseburia which could produce short chain fatty acids (SCFAs), were significantly decreased in biliary microbiota with primary CBD stones (p < 0.05). Our study provided new insights into the compositional of normal biliary microbiota. The micro-ecology of biliary and duodenal in patients with stones is complex and closely related, and there is a potential for dysbacteriosis. The decrease in abundance of certain major acid-producing bacteria affects the health of the biliary tract and thus leads to the formation of stones.Zhitang LyuTingting YuLichao ZhangXiaona XuYijun ZhangJihong LiZhirong LiWei ZhangSenlin HouKeAi Communications Co., Ltd.articlePrimary common bile duct stonesERCPDysbacteriosisBile duct microbiotaDuodenal microbiota16S rRNA gene high-throughput sequencingBiotechnologyTP248.13-248.65Biology (General)QH301-705.5ENSynthetic and Systems Biotechnology, Vol 6, Iss 4, Pp 414-428 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Primary common bile duct stones ERCP Dysbacteriosis Bile duct microbiota Duodenal microbiota 16S rRNA gene high-throughput sequencing Biotechnology TP248.13-248.65 Biology (General) QH301-705.5
spellingShingle	Primary common bile duct stones ERCP Dysbacteriosis Bile duct microbiota Duodenal microbiota 16S rRNA gene high-throughput sequencing Biotechnology TP248.13-248.65 Biology (General) QH301-705.5 Zhitang Lyu Tingting Yu Lichao Zhang Xiaona Xu Yijun Zhang Jihong Li Zhirong Li Wei Zhang Senlin Hou Analysis of the relationship between bile duct and duodenal microbiota reveals that potential dysbacteriosis is the main cause of primary common bile duct stones
description	Bacteria play an important role in the formation of primary Common Bile Duct (CBD) stones. However, the composition and function of the microbiota of bile duct in patients with primary CBD stones remained to be explored. We utilized the 16S rRNA gene high-throughput sequencing technology to analyze the microbial diversity and community composition of biliary and duodenal microbiota in 15 patients with primary CBD stones and 4 patients without biliary tract diseases. Alpha diversity analysis showed that the microbiota richness was similar in bile and intestinal fluid; Beta diversity analysis showed that there were differences in the composition between biliary microbiota and the duodenal microbiota, but the abundance of the main groups showed similarities. The composition of the biliary microbiota from gallstone patients was more complex, as was the duodenal microbiota. Proteobacteria and Firmicutes were the dominant bacteria at phylum level, accounting for at least 75% of the total reads in each subgroup. Pseudomonas and Escherichia−Shigella were the major genus among subgroups, but Escherichia-Shigella had increased abundance in duodenal microbiota with primary choledocholithiasis, which may play an important role in stone formation. It is noteworthy that Clostridiumsensu_stricto, Lachnospiraceae _UCG-008, Butyrivibrio and Roseburia which could produce short chain fatty acids (SCFAs), were significantly decreased in biliary microbiota with primary CBD stones (p < 0.05). Our study provided new insights into the compositional of normal biliary microbiota. The micro-ecology of biliary and duodenal in patients with stones is complex and closely related, and there is a potential for dysbacteriosis. The decrease in abundance of certain major acid-producing bacteria affects the health of the biliary tract and thus leads to the formation of stones.
format	article
author	Zhitang Lyu Tingting Yu Lichao Zhang Xiaona Xu Yijun Zhang Jihong Li Zhirong Li Wei Zhang Senlin Hou
author_facet	Zhitang Lyu Tingting Yu Lichao Zhang Xiaona Xu Yijun Zhang Jihong Li Zhirong Li Wei Zhang Senlin Hou
author_sort	Zhitang Lyu
title	Analysis of the relationship between bile duct and duodenal microbiota reveals that potential dysbacteriosis is the main cause of primary common bile duct stones
title_short	Analysis of the relationship between bile duct and duodenal microbiota reveals that potential dysbacteriosis is the main cause of primary common bile duct stones
title_full	Analysis of the relationship between bile duct and duodenal microbiota reveals that potential dysbacteriosis is the main cause of primary common bile duct stones
title_fullStr	Analysis of the relationship between bile duct and duodenal microbiota reveals that potential dysbacteriosis is the main cause of primary common bile duct stones
title_full_unstemmed	Analysis of the relationship between bile duct and duodenal microbiota reveals that potential dysbacteriosis is the main cause of primary common bile duct stones
title_sort	analysis of the relationship between bile duct and duodenal microbiota reveals that potential dysbacteriosis is the main cause of primary common bile duct stones
publisher	KeAi Communications Co., Ltd.
publishDate	2021
url	https://doaj.org/article/f26084fc5ae74b7fbee5bf7279d275eb
work_keys_str_mv	AT zhitanglyu analysisoftherelationshipbetweenbileductandduodenalmicrobiotarevealsthatpotentialdysbacteriosisisthemaincauseofprimarycommonbileductstones AT tingtingyu analysisoftherelationshipbetweenbileductandduodenalmicrobiotarevealsthatpotentialdysbacteriosisisthemaincauseofprimarycommonbileductstones AT lichaozhang analysisoftherelationshipbetweenbileductandduodenalmicrobiotarevealsthatpotentialdysbacteriosisisthemaincauseofprimarycommonbileductstones AT xiaonaxu analysisoftherelationshipbetweenbileductandduodenalmicrobiotarevealsthatpotentialdysbacteriosisisthemaincauseofprimarycommonbileductstones AT yijunzhang analysisoftherelationshipbetweenbileductandduodenalmicrobiotarevealsthatpotentialdysbacteriosisisthemaincauseofprimarycommonbileductstones AT jihongli analysisoftherelationshipbetweenbileductandduodenalmicrobiotarevealsthatpotentialdysbacteriosisisthemaincauseofprimarycommonbileductstones AT zhirongli analysisoftherelationshipbetweenbileductandduodenalmicrobiotarevealsthatpotentialdysbacteriosisisthemaincauseofprimarycommonbileductstones AT weizhang analysisoftherelationshipbetweenbileductandduodenalmicrobiotarevealsthatpotentialdysbacteriosisisthemaincauseofprimarycommonbileductstones AT senlinhou analysisoftherelationshipbetweenbileductandduodenalmicrobiotarevealsthatpotentialdysbacteriosisisthemaincauseofprimarycommonbileductstones
_version_	1718409877133459456

Analysis of the relationship between bile duct and duodenal microbiota reveals that potential dysbacteriosis is the main cause of primary common bile duct stones

Ejemplares similares