Airborne particulate matter in an iron mining city: Characterization, cell uptake and cytotoxicity effects of nanoparticles from PM2.5, PM10 and PM20 on human lung cells

Airborne particulate matter (PM) is dispersed into the atmosphere and can internalize in the lungs during the respiratory process by passive exposure. Itabira city (Minas Gerais, Brazil) is a mining city with relevant PM emissions derived from iron exploitation, transport, and processing. In the pre...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mariana Morozesk, Iara da Costa Souza, Marisa Narciso Fernandes, Daniel Cristian Ferreira Soares
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Air quality Emerging contaminants Nanotoxicology Risk assessment Flow cytometry Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/f26132c22d494420b8c92554aa2bfd5f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f26132c22d494420b8c92554aa2bfd5f
record_format	dspace
spelling	oai:doaj.org-article:f26132c22d494420b8c92554aa2bfd5f2021-12-02T05:04:13ZAirborne particulate matter in an iron mining city: Characterization, cell uptake and cytotoxicity effects of nanoparticles from PM2.5, PM10 and PM20 on human lung cells2666-765710.1016/j.envadv.2021.100125https://doaj.org/article/f26132c22d494420b8c92554aa2bfd5f2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S266676572100096Xhttps://doaj.org/toc/2666-7657Airborne particulate matter (PM) is dispersed into the atmosphere and can internalize in the lungs during the respiratory process by passive exposure. Itabira city (Minas Gerais, Brazil) is a mining city with relevant PM emissions derived from iron exploitation, transport, and processing. In the present study, the PM2.5, PM10, and PM20 characterization from iron ore and its internalization and cytotoxicity effects were evaluated in the MRC-5 human lung cell line. PMs were characterized using scanning electron microscopy with EDS and X-ray analysis and, then, dispersed in water and analyzed by Dynamic Light Scattering and Nanosight techniques. Flow cytometry was applied to evaluate PM cellular uptake on MRC-5 cells and death induction. The results demonstrate that the PM10 mass collected were higher than those reported by the local governmental agencies and the PMs are mainly constituted by hematite and quartz. EDS analysis showed the presence of Fe, Si, Mg, Al, Zi, Ca, and emerging metallic contaminants, such as Ba and Ti, in environmental samples. Analyzed PM samples dissociate into nanoparticles (< 200 nm) in aqueous medium, demonstrating that the PM comprises aggregated nanoparticles that can be internalized in cells, leading to a decrease in cell viability. Thus, reducing PM exposure may be considered as a potential tool for reducing metal contaminants in the air and respiratory diseases. In view of the health and environmental problems caused by airborne PM pollution, this study brings new discussions aiming to improve safety standards and future investigations in this field worldwide.Mariana MorozeskIara da Costa SouzaMarisa Narciso FernandesDaniel Cristian Ferreira SoaresElsevierarticleAir qualityEmerging contaminantsNanotoxicologyRisk assessmentFlow cytometryEnvironmental sciencesGE1-350ENEnvironmental Advances, Vol 6, Iss , Pp 100125- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Air quality Emerging contaminants Nanotoxicology Risk assessment Flow cytometry Environmental sciences GE1-350
spellingShingle	Air quality Emerging contaminants Nanotoxicology Risk assessment Flow cytometry Environmental sciences GE1-350 Mariana Morozesk Iara da Costa Souza Marisa Narciso Fernandes Daniel Cristian Ferreira Soares Airborne particulate matter in an iron mining city: Characterization, cell uptake and cytotoxicity effects of nanoparticles from PM2.5, PM10 and PM20 on human lung cells
description	Airborne particulate matter (PM) is dispersed into the atmosphere and can internalize in the lungs during the respiratory process by passive exposure. Itabira city (Minas Gerais, Brazil) is a mining city with relevant PM emissions derived from iron exploitation, transport, and processing. In the present study, the PM2.5, PM10, and PM20 characterization from iron ore and its internalization and cytotoxicity effects were evaluated in the MRC-5 human lung cell line. PMs were characterized using scanning electron microscopy with EDS and X-ray analysis and, then, dispersed in water and analyzed by Dynamic Light Scattering and Nanosight techniques. Flow cytometry was applied to evaluate PM cellular uptake on MRC-5 cells and death induction. The results demonstrate that the PM10 mass collected were higher than those reported by the local governmental agencies and the PMs are mainly constituted by hematite and quartz. EDS analysis showed the presence of Fe, Si, Mg, Al, Zi, Ca, and emerging metallic contaminants, such as Ba and Ti, in environmental samples. Analyzed PM samples dissociate into nanoparticles (< 200 nm) in aqueous medium, demonstrating that the PM comprises aggregated nanoparticles that can be internalized in cells, leading to a decrease in cell viability. Thus, reducing PM exposure may be considered as a potential tool for reducing metal contaminants in the air and respiratory diseases. In view of the health and environmental problems caused by airborne PM pollution, this study brings new discussions aiming to improve safety standards and future investigations in this field worldwide.
format	article
author	Mariana Morozesk Iara da Costa Souza Marisa Narciso Fernandes Daniel Cristian Ferreira Soares
author_facet	Mariana Morozesk Iara da Costa Souza Marisa Narciso Fernandes Daniel Cristian Ferreira Soares
author_sort	Mariana Morozesk
title	Airborne particulate matter in an iron mining city: Characterization, cell uptake and cytotoxicity effects of nanoparticles from PM2.5, PM10 and PM20 on human lung cells
title_short	Airborne particulate matter in an iron mining city: Characterization, cell uptake and cytotoxicity effects of nanoparticles from PM2.5, PM10 and PM20 on human lung cells
title_full	Airborne particulate matter in an iron mining city: Characterization, cell uptake and cytotoxicity effects of nanoparticles from PM2.5, PM10 and PM20 on human lung cells
title_fullStr	Airborne particulate matter in an iron mining city: Characterization, cell uptake and cytotoxicity effects of nanoparticles from PM2.5, PM10 and PM20 on human lung cells
title_full_unstemmed	Airborne particulate matter in an iron mining city: Characterization, cell uptake and cytotoxicity effects of nanoparticles from PM2.5, PM10 and PM20 on human lung cells
title_sort	airborne particulate matter in an iron mining city: characterization, cell uptake and cytotoxicity effects of nanoparticles from pm2.5, pm10 and pm20 on human lung cells
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/f26132c22d494420b8c92554aa2bfd5f
work_keys_str_mv	AT marianamorozesk airborneparticulatematterinanironminingcitycharacterizationcelluptakeandcytotoxicityeffectsofnanoparticlesfrompm25pm10andpm20onhumanlungcells AT iaradacostasouza airborneparticulatematterinanironminingcitycharacterizationcelluptakeandcytotoxicityeffectsofnanoparticlesfrompm25pm10andpm20onhumanlungcells AT marisanarcisofernandes airborneparticulatematterinanironminingcitycharacterizationcelluptakeandcytotoxicityeffectsofnanoparticlesfrompm25pm10andpm20onhumanlungcells AT danielcristianferreirasoares airborneparticulatematterinanironminingcitycharacterizationcelluptakeandcytotoxicityeffectsofnanoparticlesfrompm25pm10andpm20onhumanlungcells
_version_	1718400688529080320

Airborne particulate matter in an iron mining city: Characterization, cell uptake and cytotoxicity effects of nanoparticles from PM2.5, PM10 and PM20 on human lung cells

Ejemplares similares