SERS-based quantitative detection of ovarian cancer prognostic factor haptoglobin

Jayakumar Perumal,1 Ghayathri Balasundaram,1 Aniza P Mahyuddin,2 Mahesh Choolani,2 Malini Olivo1,3 1Bio-Optical Imaging Group, Singapore Bioimaging Consortium, Agency for Science Technology and Research (A*STAR), Helios, Singapore; 2Departments of Obstetrics and Gynecology and Surgery, Yong Loo Li...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Perumal J, Balasundaram G, Mahyuddin AP, Choolani M, Olivo M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/f261b55edc7a4c07b52035331835ba49
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f261b55edc7a4c07b52035331835ba49
record_format	dspace
spelling	oai:doaj.org-article:f261b55edc7a4c07b52035331835ba492021-12-02T05:57:51ZSERS-based quantitative detection of ovarian cancer prognostic factor haptoglobin1178-2013https://doaj.org/article/f261b55edc7a4c07b52035331835ba492015-03-01T00:00:00Zhttp://www.dovepress.com/sers-based-quantitative-detection-of-ovarian-cancer-prognostic-factor--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Jayakumar Perumal,1 Ghayathri Balasundaram,1 Aniza P Mahyuddin,2 Mahesh Choolani,2 Malini Olivo1,3 1Bio-Optical Imaging Group, Singapore Bioimaging Consortium, Agency for Science Technology and Research (A*STAR), Helios, Singapore; 2Departments of Obstetrics and Gynecology and Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore; 3School of Physics, National University Ireland, Galway, Ireland Abstract: Surface-enhanced Raman spectroscopy (SERS) is increasingly being used for biosensing because of its high sensitivity and low detection limit, which are made possible by the unique Raman ‘fingerprint’ spectra from the biomolecules. Here we propose a novel SERS method for the fast, sensitive, and reliable quantitative analysis of haptoglobin (Hp), an acute phase plasma glycoprotein that is widely gaining application as a prognostic ovarian cancer biomarker. We exploited the peroxidase activity of the hemoglobin–haptoglobin (Hb–Hp) complex formed by the selective and specific binding of Hp to free Hb to catalyze the reaction of 3,3',5,5'-tetramethylbenzidine (TMB) substrate and hydrogen peroxide to result in the final product of strongly SERS-active TMB2+. We observed a linear increase in the SERS signal of TMB2+ with increasing concentrations of Hb–Hp complex from 50 nM to 34 µM. Based on this concentration-dependent SERS spectrum, we quantified Hp in clinical samples. We observed that our inference about the prognosis of the disease coincided with the histology data and that our method was much more sensitive than the enzyme-linked immunosorbent assay method. Keywords: SERS-based biosensing, hemoglobin–haptoglobin complex, ovarian cancer, biomarker detection, 3,3',5,5'-tetramethylbenzidine, peroxidase active substratesPerumal JBalasundaram GMahyuddin APChoolani MOlivo MDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 1831-1840 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Perumal J Balasundaram G Mahyuddin AP Choolani M Olivo M SERS-based quantitative detection of ovarian cancer prognostic factor haptoglobin
description	Jayakumar Perumal,1 Ghayathri Balasundaram,1 Aniza P Mahyuddin,2 Mahesh Choolani,2 Malini Olivo1,3 1Bio-Optical Imaging Group, Singapore Bioimaging Consortium, Agency for Science Technology and Research (A*STAR), Helios, Singapore; 2Departments of Obstetrics and Gynecology and Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore; 3School of Physics, National University Ireland, Galway, Ireland Abstract: Surface-enhanced Raman spectroscopy (SERS) is increasingly being used for biosensing because of its high sensitivity and low detection limit, which are made possible by the unique Raman ‘fingerprint’ spectra from the biomolecules. Here we propose a novel SERS method for the fast, sensitive, and reliable quantitative analysis of haptoglobin (Hp), an acute phase plasma glycoprotein that is widely gaining application as a prognostic ovarian cancer biomarker. We exploited the peroxidase activity of the hemoglobin–haptoglobin (Hb–Hp) complex formed by the selective and specific binding of Hp to free Hb to catalyze the reaction of 3,3',5,5'-tetramethylbenzidine (TMB) substrate and hydrogen peroxide to result in the final product of strongly SERS-active TMB2+. We observed a linear increase in the SERS signal of TMB2+ with increasing concentrations of Hb–Hp complex from 50 nM to 34 µM. Based on this concentration-dependent SERS spectrum, we quantified Hp in clinical samples. We observed that our inference about the prognosis of the disease coincided with the histology data and that our method was much more sensitive than the enzyme-linked immunosorbent assay method. Keywords: SERS-based biosensing, hemoglobin–haptoglobin complex, ovarian cancer, biomarker detection, 3,3',5,5'-tetramethylbenzidine, peroxidase active substrates
format	article
author	Perumal J Balasundaram G Mahyuddin AP Choolani M Olivo M
author_facet	Perumal J Balasundaram G Mahyuddin AP Choolani M Olivo M
author_sort	Perumal J
title	SERS-based quantitative detection of ovarian cancer prognostic factor haptoglobin
title_short	SERS-based quantitative detection of ovarian cancer prognostic factor haptoglobin
title_full	SERS-based quantitative detection of ovarian cancer prognostic factor haptoglobin
title_fullStr	SERS-based quantitative detection of ovarian cancer prognostic factor haptoglobin
title_full_unstemmed	SERS-based quantitative detection of ovarian cancer prognostic factor haptoglobin
title_sort	sers-based quantitative detection of ovarian cancer prognostic factor haptoglobin
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/f261b55edc7a4c07b52035331835ba49
work_keys_str_mv	AT perumalj sersbasedquantitativedetectionofovariancancerprognosticfactorhaptoglobin AT balasundaramg sersbasedquantitativedetectionofovariancancerprognosticfactorhaptoglobin AT mahyuddinap sersbasedquantitativedetectionofovariancancerprognosticfactorhaptoglobin AT choolanim sersbasedquantitativedetectionofovariancancerprognosticfactorhaptoglobin AT olivom sersbasedquantitativedetectionofovariancancerprognosticfactorhaptoglobin
_version_	1718400147540410368

SERS-based quantitative detection of ovarian cancer prognostic factor haptoglobin

Ejemplares similares