The Distribution Characteristics and Applications for Maternal Cells on Chicken Egg Vitelline Membrane

Abstract The major components of vitelline membrane (VM) are ovomucin, VM outer (VMO) I and VMO II. At present, the distribution pattern of maternal cells on the VM has not been described in detail. In this study, the existence and distribution characteristics of maternal cells on VM were observed....

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Autores principales:	Quanlin Li, Wenbo Li, Xingzheng Li, Lulu Liu, Ying Zhang, Yuying Guo, Xia Chen, Guiyun Xu, Jiangxia Zheng
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f263ae49bc264fa09c9e8cd378ff5de8
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spelling	oai:doaj.org-article:f263ae49bc264fa09c9e8cd378ff5de82021-12-02T11:52:22ZThe Distribution Characteristics and Applications for Maternal Cells on Chicken Egg Vitelline Membrane10.1038/s41598-017-06996-12045-2322https://doaj.org/article/f263ae49bc264fa09c9e8cd378ff5de82017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06996-1https://doaj.org/toc/2045-2322Abstract The major components of vitelline membrane (VM) are ovomucin, VM outer (VMO) I and VMO II. At present, the distribution pattern of maternal cells on the VM has not been described in detail. In this study, the existence and distribution characteristics of maternal cells on VM were observed. There were more than 3.2 × 105 somatic cells on VM, which were uneven distributed. The calcein AM/PI staining of the maternal cells on the VM showed that the cells’ viability changed with the freshness of the eggs, and that the maternal cells gradually underwent apoptosis and became degraded. The results of morphology of different tissues indicated that the most of maternal cells on the VM were granulosa cells. Moreover, the karyotype of the cultured granulosa cells, which is the main source of cells on VM, were identified as the normal diploid karyotype of chicken. Furthermore, the VM DNA extracted from chickens and quails, which represent the eggs of different size, was adequate for further genetic analysis. The VM DNA was easily accessible and relatively constant, without cross-contamination. Therefore, the VM DNA could potentially be applied for the molecular traceability between eggs and chickens, and be beneficial in avian ecology research studies.Quanlin LiWenbo LiXingzheng LiLulu LiuYing ZhangYuying GuoXia ChenGuiyun XuJiangxia ZhengNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Quanlin Li Wenbo Li Xingzheng Li Lulu Liu Ying Zhang Yuying Guo Xia Chen Guiyun Xu Jiangxia Zheng The Distribution Characteristics and Applications for Maternal Cells on Chicken Egg Vitelline Membrane
description	Abstract The major components of vitelline membrane (VM) are ovomucin, VM outer (VMO) I and VMO II. At present, the distribution pattern of maternal cells on the VM has not been described in detail. In this study, the existence and distribution characteristics of maternal cells on VM were observed. There were more than 3.2 × 105 somatic cells on VM, which were uneven distributed. The calcein AM/PI staining of the maternal cells on the VM showed that the cells’ viability changed with the freshness of the eggs, and that the maternal cells gradually underwent apoptosis and became degraded. The results of morphology of different tissues indicated that the most of maternal cells on the VM were granulosa cells. Moreover, the karyotype of the cultured granulosa cells, which is the main source of cells on VM, were identified as the normal diploid karyotype of chicken. Furthermore, the VM DNA extracted from chickens and quails, which represent the eggs of different size, was adequate for further genetic analysis. The VM DNA was easily accessible and relatively constant, without cross-contamination. Therefore, the VM DNA could potentially be applied for the molecular traceability between eggs and chickens, and be beneficial in avian ecology research studies.
format	article
author	Quanlin Li Wenbo Li Xingzheng Li Lulu Liu Ying Zhang Yuying Guo Xia Chen Guiyun Xu Jiangxia Zheng
author_facet	Quanlin Li Wenbo Li Xingzheng Li Lulu Liu Ying Zhang Yuying Guo Xia Chen Guiyun Xu Jiangxia Zheng
author_sort	Quanlin Li
title	The Distribution Characteristics and Applications for Maternal Cells on Chicken Egg Vitelline Membrane
title_short	The Distribution Characteristics and Applications for Maternal Cells on Chicken Egg Vitelline Membrane
title_full	The Distribution Characteristics and Applications for Maternal Cells on Chicken Egg Vitelline Membrane
title_fullStr	The Distribution Characteristics and Applications for Maternal Cells on Chicken Egg Vitelline Membrane
title_full_unstemmed	The Distribution Characteristics and Applications for Maternal Cells on Chicken Egg Vitelline Membrane
title_sort	distribution characteristics and applications for maternal cells on chicken egg vitelline membrane
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f263ae49bc264fa09c9e8cd378ff5de8
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The Distribution Characteristics and Applications for Maternal Cells on Chicken Egg Vitelline Membrane

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