Understanding improved osteoblast behavior on select nanoporous anodic alumina

Siyu Ni,1 Changyan Li,1 Shirong Ni,2 Ting Chen,1 Thomas J Webster3,4 1College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, People’s Republic of China; 2Department of Pathophysiology, Wenzhou Medical University, Wenzhou, People’s Republic o...

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Autores principales:	Ni S, Li C, Chen T, Webster TJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/f267e2df5c8d4f0ba8d40410396319b3
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spelling	oai:doaj.org-article:f267e2df5c8d4f0ba8d40410396319b32021-12-02T01:16:06ZUnderstanding improved osteoblast behavior on select nanoporous anodic alumina1178-2013https://doaj.org/article/f267e2df5c8d4f0ba8d40410396319b32014-07-01T00:00:00Zhttp://www.dovepress.com/understanding-improved-osteoblast-behavior-on-select-nanoporous-anodic-a17555https://doaj.org/toc/1178-2013 Siyu Ni,1 Changyan Li,1 Shirong Ni,2 Ting Chen,1 Thomas J Webster3,4 1College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, People’s Republic of China; 2Department of Pathophysiology, Wenzhou Medical University, Wenzhou, People’s Republic of China; 3Department of Chemical Engineering, College of Engineering, Northeastern University, Boston, MA, USA; 4Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Saudi Arabia Abstract: The aim of this study was to prepare different sized porous anodic alumina (PAA) and examine preosteoblast (MC3T3-E1) attachment and proliferation on such nanoporous surfaces. In this study, PAA with tunable pore sizes (25 nm, 50 nm, and 75 nm) were fabricated by a two-step anodizing procedure in oxalic acid. The surface morphology and elemental composition of PAA were characterized by field emission scanning electron microscopy and X-ray photoelectron spectroscopy analysis. The nanopore arrays on all of the PAA samples were highly regular. X-ray photoelectron spectroscopy analysis suggested that the chemistry of PAA and flat aluminum surfaces were similar. However, contact angles were significantly greater on all of the PAA compared to flat aluminum substrates, which consequently altered protein adsorption profiles. The attachment and proliferation of preosteoblasts were determined for up to 7 days in culture using field emission scanning electron microscopy and a Cell Counting Kit-8. Results showed that nanoporous surfaces did not enhance initial preosteoblast attachment, whereas preosteoblast proliferation dramatically increased when the PAA pore size was either 50 nm or 75 nm compared to all other samples (P<0.05). Thus, this study showed that one can alter surface energy of aluminum by modifying surface nano-roughness alone (and not changing chemistry) through an anodization process to improve osteoblast density, and, thus, should be further studied as a bioactive interface for orthopedic applications. Keywords: nanostructure, adhesion, proliferation, preosteoblast, orthopedicsNi SLi CNi SChen TWebster TJDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 3325-3334 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Ni S Li C Ni S Chen T Webster TJ Understanding improved osteoblast behavior on select nanoporous anodic alumina
description	Siyu Ni,1 Changyan Li,1 Shirong Ni,2 Ting Chen,1 Thomas J Webster3,4 1College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, People’s Republic of China; 2Department of Pathophysiology, Wenzhou Medical University, Wenzhou, People’s Republic of China; 3Department of Chemical Engineering, College of Engineering, Northeastern University, Boston, MA, USA; 4Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Saudi Arabia Abstract: The aim of this study was to prepare different sized porous anodic alumina (PAA) and examine preosteoblast (MC3T3-E1) attachment and proliferation on such nanoporous surfaces. In this study, PAA with tunable pore sizes (25 nm, 50 nm, and 75 nm) were fabricated by a two-step anodizing procedure in oxalic acid. The surface morphology and elemental composition of PAA were characterized by field emission scanning electron microscopy and X-ray photoelectron spectroscopy analysis. The nanopore arrays on all of the PAA samples were highly regular. X-ray photoelectron spectroscopy analysis suggested that the chemistry of PAA and flat aluminum surfaces were similar. However, contact angles were significantly greater on all of the PAA compared to flat aluminum substrates, which consequently altered protein adsorption profiles. The attachment and proliferation of preosteoblasts were determined for up to 7 days in culture using field emission scanning electron microscopy and a Cell Counting Kit-8. Results showed that nanoporous surfaces did not enhance initial preosteoblast attachment, whereas preosteoblast proliferation dramatically increased when the PAA pore size was either 50 nm or 75 nm compared to all other samples (P<0.05). Thus, this study showed that one can alter surface energy of aluminum by modifying surface nano-roughness alone (and not changing chemistry) through an anodization process to improve osteoblast density, and, thus, should be further studied as a bioactive interface for orthopedic applications. Keywords: nanostructure, adhesion, proliferation, preosteoblast, orthopedics
format	article
author	Ni S Li C Ni S Chen T Webster TJ
author_facet	Ni S Li C Ni S Chen T Webster TJ
author_sort	Ni S
title	Understanding improved osteoblast behavior on select nanoporous anodic alumina
title_short	Understanding improved osteoblast behavior on select nanoporous anodic alumina
title_full	Understanding improved osteoblast behavior on select nanoporous anodic alumina
title_fullStr	Understanding improved osteoblast behavior on select nanoporous anodic alumina
title_full_unstemmed	Understanding improved osteoblast behavior on select nanoporous anodic alumina
title_sort	understanding improved osteoblast behavior on select nanoporous anodic alumina
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/f267e2df5c8d4f0ba8d40410396319b3
work_keys_str_mv	AT nis understandingimprovedosteoblastbehavioronselectnanoporousanodicalumina AT lic understandingimprovedosteoblastbehavioronselectnanoporousanodicalumina AT nis understandingimprovedosteoblastbehavioronselectnanoporousanodicalumina AT chent understandingimprovedosteoblastbehavioronselectnanoporousanodicalumina AT webstertj understandingimprovedosteoblastbehavioronselectnanoporousanodicalumina
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Understanding improved osteoblast behavior on select nanoporous anodic alumina

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