LINC01006 regulates the proliferation, migration and invasion of hepatocellular carcinoma cells through regulating miR-433-3p/CBX3 axis

Introduction and objectives: LINC01006 has been verified to be correlated with several cancer types, whereas its biological function in hepatocellular carcinoma (HCC) is still elusive. This study aimed to elucidate the specific regulatory mechanism of LINC01006 in the tumorigenesis of HCC. Materials...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yaobo Song, Shuang Wang, Xiangming Cheng
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Hepatocellular carcinoma Viability Migration Long intergenic non-protein coding RNA 01006 MicroRNA-433-3p Chromobox protein homolog 3 Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/f28b97bc175f43bfa19f3e29fe06d4b6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f28b97bc175f43bfa19f3e29fe06d4b6
record_format	dspace
spelling	oai:doaj.org-article:f28b97bc175f43bfa19f3e29fe06d4b62021-11-18T04:46:01ZLINC01006 regulates the proliferation, migration and invasion of hepatocellular carcinoma cells through regulating miR-433-3p/CBX3 axis1665-268110.1016/j.aohep.2021.100343https://doaj.org/article/f28b97bc175f43bfa19f3e29fe06d4b62021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1665268121000429https://doaj.org/toc/1665-2681Introduction and objectives: LINC01006 has been verified to be correlated with several cancer types, whereas its biological function in hepatocellular carcinoma (HCC) is still elusive. This study aimed to elucidate the specific regulatory mechanism of LINC01006 in the tumorigenesis of HCC. Materials and methods: The expression of LINC01006, miR-433-3p and CBX3 in HCC tissues and cells was assessed by qRT-PCR or Western blot. MTT, wound-healing, and transwell assays were used to evaluate the effects of LINC01006 on cell viability, migration, and invasion in vitro. A mouse xenograft model was established for in vivo assays. The relations among LINC01006, miR-433-3p, and CBX3 were analyzed by MS2-RNA immunoprecipitation (RIP) and Dual-luciferase reporter (DLR) assays. Results: The expression of LINC01006 was up-regulated in HCC tissues and cells. LINC01006 knockdown inhibited the viability, wound healing rate, and invasive cell number of HeP3B and SK-HeP-1 cells, and decreased the tumor volume and weight in a mouse xenograft model. MiR-433-3p was a target of LINC01006, and LINC01006 overexpression inhibited the viability, wound healing rate, and invasive cell number of HeP3B and SK-HeP-1 cells. In addition, CBX3 was a target of miR-433-3p, which was negatively regulated by miR-433-3p. CBX3 overexpression and miR-433-3p inhibition reversed the inhibiting effects of LINC01006 knockdown on the viability, migration, and invasion of HeP3B cells. Conclusions: Silencing of LINC01006 inhibited the viability, migration, and invasion of HCC cells through regulating miR-433-3p/CBX3 axis.Yaobo SongShuang WangXiangming ChengElsevierarticleHepatocellular carcinomaViabilityMigrationLong intergenic non-protein coding RNA 01006MicroRNA-433-3pChromobox protein homolog 3Specialties of internal medicineRC581-951ENAnnals of Hepatology, Vol 25, Iss , Pp 100343- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Hepatocellular carcinoma Viability Migration Long intergenic non-protein coding RNA 01006 MicroRNA-433-3p Chromobox protein homolog 3 Specialties of internal medicine RC581-951
spellingShingle	Hepatocellular carcinoma Viability Migration Long intergenic non-protein coding RNA 01006 MicroRNA-433-3p Chromobox protein homolog 3 Specialties of internal medicine RC581-951 Yaobo Song Shuang Wang Xiangming Cheng LINC01006 regulates the proliferation, migration and invasion of hepatocellular carcinoma cells through regulating miR-433-3p/CBX3 axis
description	Introduction and objectives: LINC01006 has been verified to be correlated with several cancer types, whereas its biological function in hepatocellular carcinoma (HCC) is still elusive. This study aimed to elucidate the specific regulatory mechanism of LINC01006 in the tumorigenesis of HCC. Materials and methods: The expression of LINC01006, miR-433-3p and CBX3 in HCC tissues and cells was assessed by qRT-PCR or Western blot. MTT, wound-healing, and transwell assays were used to evaluate the effects of LINC01006 on cell viability, migration, and invasion in vitro. A mouse xenograft model was established for in vivo assays. The relations among LINC01006, miR-433-3p, and CBX3 were analyzed by MS2-RNA immunoprecipitation (RIP) and Dual-luciferase reporter (DLR) assays. Results: The expression of LINC01006 was up-regulated in HCC tissues and cells. LINC01006 knockdown inhibited the viability, wound healing rate, and invasive cell number of HeP3B and SK-HeP-1 cells, and decreased the tumor volume and weight in a mouse xenograft model. MiR-433-3p was a target of LINC01006, and LINC01006 overexpression inhibited the viability, wound healing rate, and invasive cell number of HeP3B and SK-HeP-1 cells. In addition, CBX3 was a target of miR-433-3p, which was negatively regulated by miR-433-3p. CBX3 overexpression and miR-433-3p inhibition reversed the inhibiting effects of LINC01006 knockdown on the viability, migration, and invasion of HeP3B cells. Conclusions: Silencing of LINC01006 inhibited the viability, migration, and invasion of HCC cells through regulating miR-433-3p/CBX3 axis.
format	article
author	Yaobo Song Shuang Wang Xiangming Cheng
author_facet	Yaobo Song Shuang Wang Xiangming Cheng
author_sort	Yaobo Song
title	LINC01006 regulates the proliferation, migration and invasion of hepatocellular carcinoma cells through regulating miR-433-3p/CBX3 axis
title_short	LINC01006 regulates the proliferation, migration and invasion of hepatocellular carcinoma cells through regulating miR-433-3p/CBX3 axis
title_full	LINC01006 regulates the proliferation, migration and invasion of hepatocellular carcinoma cells through regulating miR-433-3p/CBX3 axis
title_fullStr	LINC01006 regulates the proliferation, migration and invasion of hepatocellular carcinoma cells through regulating miR-433-3p/CBX3 axis
title_full_unstemmed	LINC01006 regulates the proliferation, migration and invasion of hepatocellular carcinoma cells through regulating miR-433-3p/CBX3 axis
title_sort	linc01006 regulates the proliferation, migration and invasion of hepatocellular carcinoma cells through regulating mir-433-3p/cbx3 axis
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/f28b97bc175f43bfa19f3e29fe06d4b6
work_keys_str_mv	AT yaobosong linc01006regulatestheproliferationmigrationandinvasionofhepatocellularcarcinomacellsthroughregulatingmir4333pcbx3axis AT shuangwang linc01006regulatestheproliferationmigrationandinvasionofhepatocellularcarcinomacellsthroughregulatingmir4333pcbx3axis AT xiangmingcheng linc01006regulatestheproliferationmigrationandinvasionofhepatocellularcarcinomacellsthroughregulatingmir4333pcbx3axis
_version_	1718425074971705344

LINC01006 regulates the proliferation, migration and invasion of hepatocellular carcinoma cells through regulating miR-433-3p/CBX3 axis

Ejemplares similares