BASOPHILE ACTIVATION TEST FOR THE DIAGNOSTICS OF FUNGAL SENSITIZATION IN THE PATIENTS WITH CYSTIC FIBROSIS
Aspergillus fumigatus colonization in the patients with cystic fibrosis (CF) may cause sensitization against A. fumigatus and/or allergic bronchopulmonary aspergillosis (ABPA), which significantly worsens the course of underlying disease. At the present time, new diagnostic tests are searched for de...
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oai:doaj.org-article:f29a786a592f4676bdf0ba3fccca289e2021-11-18T08:03:49ZBASOPHILE ACTIVATION TEST FOR THE DIAGNOSTICS OF FUNGAL SENSITIZATION IN THE PATIENTS WITH CYSTIC FIBROSIS1563-06252313-741X10.15789/1563-0625-2019-5-919-928https://doaj.org/article/f29a786a592f4676bdf0ba3fccca289e2019-12-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1693https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAspergillus fumigatus colonization in the patients with cystic fibrosis (CF) may cause sensitization against A. fumigatus and/or allergic bronchopulmonary aspergillosis (ABPA), which significantly worsens the course of underlying disease. At the present time, new diagnostic tests are searched for detection of fungal sensitization in these patients. The aim of this work was to evaluate an opportunity of application of basophile activation test with A. fumigatus allergen in vitro using flow cytometry, aiming for identification of fungal sensitization in the CF patients. The study included 190 patients with CF aged 1 to 37 years. All the patients underwent common allergy screening (skin tests with fungal allergens, determination of serum levels of total IgE and specific IgE for the fungal allergens), and mycological examination (microscopy and culture of respiratory substrates). Computed tomography of the chest was performed upon clinical indications. The basophil activation test with the A. fumigatus allergen was performed in 10 CF patients with ABPA, and 10 CF patients without ABPA, in addition to the standard allergological examination. Frequency of sensitization to A. fumigatus in the patients with cystic fibrosis was 27%, the incidence of allergic bronchopulmonary aspergillosis was 5.7%. The number of eosinophils, total IgE and specific IgE levels in CF patients with ABPA were significantly higher than in CF patients without ABPA. In blood of the ABPA patients we have identified 68.5 (52.5-81.5%) of basophilic leukocytes activated by A. fumigatus allergen, with a stimulation index of 17.07 (10.30-27.70). In appropriate comparison group, the stimulation index did not exceed 1.5 (p = 0.000). Direct positive correlation between the levels of specific IgE to A. fumigatus and the number of basophils activated by A. fumigatus allergens was revealed (r = 0.77; р < 0.05). FVC values and the body mass index in CF patients with ABPA were significantly lower when compared with the patients without fungal sensitization. Introduction of the basophil activation test, along with standard techniques, may enable a more differentiated assessment of ABPA development in CF patients. Timely detection of associations between A. fumigatus sensitization and clinical status of CF patients will facilitate early and effective administration of specific therapy.Ya. I. KozlovaE. V. FrolovaA. E. UchevatkinaL. V. FilippovaO. V. AakT. S. BogomolovaYu. V. BorzovaV. R. MakhmutovaT. A. StepanenkoV. D. KuznetsovN. V. VasilyevaM. V. ShulginaN. N. KlimkoSPb RAACIarticlebasophil activation testaspergillus spp.aspergillus fumigatusallergic bronchopulmonary aspergillosiscystic fibrosisfungal sensitizationImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 21, Iss 5, Pp 919-928 (2019) |
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| collection |
DOAJ |
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RU |
| topic |
basophil activation test aspergillus spp. aspergillus fumigatus allergic bronchopulmonary aspergillosis cystic fibrosis fungal sensitization Immunologic diseases. Allergy RC581-607 |
| spellingShingle |
basophil activation test aspergillus spp. aspergillus fumigatus allergic bronchopulmonary aspergillosis cystic fibrosis fungal sensitization Immunologic diseases. Allergy RC581-607 Ya. I. Kozlova E. V. Frolova A. E. Uchevatkina L. V. Filippova O. V. Aak T. S. Bogomolova Yu. V. Borzova V. R. Makhmutova T. A. Stepanenko V. D. Kuznetsov N. V. Vasilyeva M. V. Shulgina N. N. Klimko BASOPHILE ACTIVATION TEST FOR THE DIAGNOSTICS OF FUNGAL SENSITIZATION IN THE PATIENTS WITH CYSTIC FIBROSIS |
| description |
Aspergillus fumigatus colonization in the patients with cystic fibrosis (CF) may cause sensitization against A. fumigatus and/or allergic bronchopulmonary aspergillosis (ABPA), which significantly worsens the course of underlying disease. At the present time, new diagnostic tests are searched for detection of fungal sensitization in these patients. The aim of this work was to evaluate an opportunity of application of basophile activation test with A. fumigatus allergen in vitro using flow cytometry, aiming for identification of fungal sensitization in the CF patients. The study included 190 patients with CF aged 1 to 37 years. All the patients underwent common allergy screening (skin tests with fungal allergens, determination of serum levels of total IgE and specific IgE for the fungal allergens), and mycological examination (microscopy and culture of respiratory substrates). Computed tomography of the chest was performed upon clinical indications. The basophil activation test with the A. fumigatus allergen was performed in 10 CF patients with ABPA, and 10 CF patients without ABPA, in addition to the standard allergological examination. Frequency of sensitization to A. fumigatus in the patients with cystic fibrosis was 27%, the incidence of allergic bronchopulmonary aspergillosis was 5.7%. The number of eosinophils, total IgE and specific IgE levels in CF patients with ABPA were significantly higher than in CF patients without ABPA. In blood of the ABPA patients we have identified 68.5 (52.5-81.5%) of basophilic leukocytes activated by A. fumigatus allergen, with a stimulation index of 17.07 (10.30-27.70). In appropriate comparison group, the stimulation index did not exceed 1.5 (p = 0.000). Direct positive correlation between the levels of specific IgE to A. fumigatus and the number of basophils activated by A. fumigatus allergens was revealed (r = 0.77; р < 0.05). FVC values and the body mass index in CF patients with ABPA were significantly lower when compared with the patients without fungal sensitization. Introduction of the basophil activation test, along with standard techniques, may enable a more differentiated assessment of ABPA development in CF patients. Timely detection of associations between A. fumigatus sensitization and clinical status of CF patients will facilitate early and effective administration of specific therapy. |
| format |
article |
| author |
Ya. I. Kozlova E. V. Frolova A. E. Uchevatkina L. V. Filippova O. V. Aak T. S. Bogomolova Yu. V. Borzova V. R. Makhmutova T. A. Stepanenko V. D. Kuznetsov N. V. Vasilyeva M. V. Shulgina N. N. Klimko |
| author_facet |
Ya. I. Kozlova E. V. Frolova A. E. Uchevatkina L. V. Filippova O. V. Aak T. S. Bogomolova Yu. V. Borzova V. R. Makhmutova T. A. Stepanenko V. D. Kuznetsov N. V. Vasilyeva M. V. Shulgina N. N. Klimko |
| author_sort |
Ya. I. Kozlova |
| title |
BASOPHILE ACTIVATION TEST FOR THE DIAGNOSTICS OF FUNGAL SENSITIZATION IN THE PATIENTS WITH CYSTIC FIBROSIS |
| title_short |
BASOPHILE ACTIVATION TEST FOR THE DIAGNOSTICS OF FUNGAL SENSITIZATION IN THE PATIENTS WITH CYSTIC FIBROSIS |
| title_full |
BASOPHILE ACTIVATION TEST FOR THE DIAGNOSTICS OF FUNGAL SENSITIZATION IN THE PATIENTS WITH CYSTIC FIBROSIS |
| title_fullStr |
BASOPHILE ACTIVATION TEST FOR THE DIAGNOSTICS OF FUNGAL SENSITIZATION IN THE PATIENTS WITH CYSTIC FIBROSIS |
| title_full_unstemmed |
BASOPHILE ACTIVATION TEST FOR THE DIAGNOSTICS OF FUNGAL SENSITIZATION IN THE PATIENTS WITH CYSTIC FIBROSIS |
| title_sort |
basophile activation test for the diagnostics of fungal sensitization in the patients with cystic fibrosis |
| publisher |
SPb RAACI |
| publishDate |
2019 |
| url |
https://doaj.org/article/f29a786a592f4676bdf0ba3fccca289e |
| work_keys_str_mv |
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| _version_ |
1718422328330682368 |