Distinct DNA exit and packaging portals in the virus Acanthamoeba polyphaga mimivirus.

Icosahedral double-stranded DNA viruses use a single portal for genome delivery and packaging. The extensive structural similarity revealed by such portals in diverse viruses, as well as their invariable positioning at a unique icosahedral vertex, led to the consensus that a particular, highly conse...

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Autores principales:	Nathan Zauberman, Yael Mutsafi, Daniel Ben Halevy, Eyal Shimoni, Eugenia Klein, Chuan Xiao, Siyang Sun, Abraham Minsky
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f29ccfd8009d47679e1c06a52d9418e2
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spelling	oai:doaj.org-article:f29ccfd8009d47679e1c06a52d9418e22021-11-25T05:33:21ZDistinct DNA exit and packaging portals in the virus Acanthamoeba polyphaga mimivirus.1544-91731545-788510.1371/journal.pbio.0060114https://doaj.org/article/f29ccfd8009d47679e1c06a52d9418e22008-05-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18479185/pdf/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Icosahedral double-stranded DNA viruses use a single portal for genome delivery and packaging. The extensive structural similarity revealed by such portals in diverse viruses, as well as their invariable positioning at a unique icosahedral vertex, led to the consensus that a particular, highly conserved vertex-portal architecture is essential for viral DNA translocations. Here we present an exception to this paradigm by demonstrating that genome delivery and packaging in the virus Acanthamoeba polyphaga mimivirus occur through two distinct portals. By using high-resolution techniques, including electron tomography and cryo-scanning electron microscopy, we show that Mimivirus genome delivery entails a large-scale conformational change of the capsid, whereby five icosahedral faces open up. This opening, which occurs at a unique vertex of the capsid that we coined the "stargate", allows for the formation of a massive membrane conduit through which the viral DNA is released. A transient aperture centered at an icosahedral face distal to the DNA delivery site acts as a non-vertex DNA packaging portal. In conjunction with comparative genomic studies, our observations imply a viral packaging pathway akin to bacterial DNA segregation, which might be shared by diverse internal membrane-containing viruses.Nathan ZaubermanYael MutsafiDaniel Ben HalevyEyal ShimoniEugenia KleinChuan XiaoSiyang SunAbraham MinskyPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 6, Iss 5, p e114 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Nathan Zauberman Yael Mutsafi Daniel Ben Halevy Eyal Shimoni Eugenia Klein Chuan Xiao Siyang Sun Abraham Minsky Distinct DNA exit and packaging portals in the virus Acanthamoeba polyphaga mimivirus.
description	Icosahedral double-stranded DNA viruses use a single portal for genome delivery and packaging. The extensive structural similarity revealed by such portals in diverse viruses, as well as their invariable positioning at a unique icosahedral vertex, led to the consensus that a particular, highly conserved vertex-portal architecture is essential for viral DNA translocations. Here we present an exception to this paradigm by demonstrating that genome delivery and packaging in the virus Acanthamoeba polyphaga mimivirus occur through two distinct portals. By using high-resolution techniques, including electron tomography and cryo-scanning electron microscopy, we show that Mimivirus genome delivery entails a large-scale conformational change of the capsid, whereby five icosahedral faces open up. This opening, which occurs at a unique vertex of the capsid that we coined the "stargate", allows for the formation of a massive membrane conduit through which the viral DNA is released. A transient aperture centered at an icosahedral face distal to the DNA delivery site acts as a non-vertex DNA packaging portal. In conjunction with comparative genomic studies, our observations imply a viral packaging pathway akin to bacterial DNA segregation, which might be shared by diverse internal membrane-containing viruses.
format	article
author	Nathan Zauberman Yael Mutsafi Daniel Ben Halevy Eyal Shimoni Eugenia Klein Chuan Xiao Siyang Sun Abraham Minsky
author_facet	Nathan Zauberman Yael Mutsafi Daniel Ben Halevy Eyal Shimoni Eugenia Klein Chuan Xiao Siyang Sun Abraham Minsky
author_sort	Nathan Zauberman
title	Distinct DNA exit and packaging portals in the virus Acanthamoeba polyphaga mimivirus.
title_short	Distinct DNA exit and packaging portals in the virus Acanthamoeba polyphaga mimivirus.
title_full	Distinct DNA exit and packaging portals in the virus Acanthamoeba polyphaga mimivirus.
title_fullStr	Distinct DNA exit and packaging portals in the virus Acanthamoeba polyphaga mimivirus.
title_full_unstemmed	Distinct DNA exit and packaging portals in the virus Acanthamoeba polyphaga mimivirus.
title_sort	distinct dna exit and packaging portals in the virus acanthamoeba polyphaga mimivirus.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/f29ccfd8009d47679e1c06a52d9418e2
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Distinct DNA exit and packaging portals in the virus Acanthamoeba polyphaga mimivirus.

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