Clinical and Molecular Epidemiology of Human Parainfluenza Viruses 1–4 in Children from Viet Nam

Abstract HPIVs are serologically and genetically grouped into four species that account for up to 10% of all hospitalizations due to acute respiratory infection in children under the age of five. Genetic and epidemiological data for the four HPIVs derived from two pediatric cohorts in Viet Nam are p...

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Autores principales:	Martin Linster, Lien Anh Ha Do, Ngo Ngoc Quang Minh, Yihui Chen, Zhu Zhe, Tran Anh Tuan, Ha Manh Tuan, Yvonne C. F. Su, H. Rogier van Doorn, Mahesh Moorthy, Gavin J. D. Smith
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f2a49baff42948cabfdeda8b51957ca1
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spelling	oai:doaj.org-article:f2a49baff42948cabfdeda8b51957ca12021-12-02T16:07:50ZClinical and Molecular Epidemiology of Human Parainfluenza Viruses 1–4 in Children from Viet Nam10.1038/s41598-018-24767-42045-2322https://doaj.org/article/f2a49baff42948cabfdeda8b51957ca12018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-24767-4https://doaj.org/toc/2045-2322Abstract HPIVs are serologically and genetically grouped into four species that account for up to 10% of all hospitalizations due to acute respiratory infection in children under the age of five. Genetic and epidemiological data for the four HPIVs derived from two pediatric cohorts in Viet Nam are presented. Respiratory samples were screened for HPIV1–4 by real-time PCR. Demographic and clinical data of patients infected with different HPIV were compared. We used a hemi-nested PCR approach to generate viral genome sequences from HPIV-positive samples and conducted a comprehensive phylogenetic analysis. In total, 170 samples tested positive for HPIV. HPIV3 was most commonly detected in our cohort and 80 co-detections of HPIV with other respiratory viruses were found. Phylogenetic analyses suggest local endemic circulation as well as punctuated introductions of new HPIV lineages. Viral gene flow analysis revealed that Viet Nam is a net importer of viral genetic diversity. Epidemiological analyses imply similar disease severity for all HPIV species. HPIV sequences from Viet Nam formed local clusters and were interspersed with sequences from diverse geographic regions. Combined, this new knowledge will help to investigate global HPIV circulation patterns in more detail and ultimately define more suitable vaccine strains.Martin LinsterLien Anh Ha DoNgo Ngoc Quang MinhYihui ChenZhu ZheTran Anh TuanHa Manh TuanYvonne C. F. SuH. Rogier van DoornMahesh MoorthyGavin J. D. SmithNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-8 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Martin Linster Lien Anh Ha Do Ngo Ngoc Quang Minh Yihui Chen Zhu Zhe Tran Anh Tuan Ha Manh Tuan Yvonne C. F. Su H. Rogier van Doorn Mahesh Moorthy Gavin J. D. Smith Clinical and Molecular Epidemiology of Human Parainfluenza Viruses 1–4 in Children from Viet Nam
description	Abstract HPIVs are serologically and genetically grouped into four species that account for up to 10% of all hospitalizations due to acute respiratory infection in children under the age of five. Genetic and epidemiological data for the four HPIVs derived from two pediatric cohorts in Viet Nam are presented. Respiratory samples were screened for HPIV1–4 by real-time PCR. Demographic and clinical data of patients infected with different HPIV were compared. We used a hemi-nested PCR approach to generate viral genome sequences from HPIV-positive samples and conducted a comprehensive phylogenetic analysis. In total, 170 samples tested positive for HPIV. HPIV3 was most commonly detected in our cohort and 80 co-detections of HPIV with other respiratory viruses were found. Phylogenetic analyses suggest local endemic circulation as well as punctuated introductions of new HPIV lineages. Viral gene flow analysis revealed that Viet Nam is a net importer of viral genetic diversity. Epidemiological analyses imply similar disease severity for all HPIV species. HPIV sequences from Viet Nam formed local clusters and were interspersed with sequences from diverse geographic regions. Combined, this new knowledge will help to investigate global HPIV circulation patterns in more detail and ultimately define more suitable vaccine strains.
format	article
author	Martin Linster Lien Anh Ha Do Ngo Ngoc Quang Minh Yihui Chen Zhu Zhe Tran Anh Tuan Ha Manh Tuan Yvonne C. F. Su H. Rogier van Doorn Mahesh Moorthy Gavin J. D. Smith
author_facet	Martin Linster Lien Anh Ha Do Ngo Ngoc Quang Minh Yihui Chen Zhu Zhe Tran Anh Tuan Ha Manh Tuan Yvonne C. F. Su H. Rogier van Doorn Mahesh Moorthy Gavin J. D. Smith
author_sort	Martin Linster
title	Clinical and Molecular Epidemiology of Human Parainfluenza Viruses 1–4 in Children from Viet Nam
title_short	Clinical and Molecular Epidemiology of Human Parainfluenza Viruses 1–4 in Children from Viet Nam
title_full	Clinical and Molecular Epidemiology of Human Parainfluenza Viruses 1–4 in Children from Viet Nam
title_fullStr	Clinical and Molecular Epidemiology of Human Parainfluenza Viruses 1–4 in Children from Viet Nam
title_full_unstemmed	Clinical and Molecular Epidemiology of Human Parainfluenza Viruses 1–4 in Children from Viet Nam
title_sort	clinical and molecular epidemiology of human parainfluenza viruses 1–4 in children from viet nam
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/f2a49baff42948cabfdeda8b51957ca1
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Clinical and Molecular Epidemiology of Human Parainfluenza Viruses 1–4 in Children from Viet Nam

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