Comprehensive assessment of specific antibodies on infectious activity of tick-borne encephalitis virus

Vaccines for prophylactic immunization provide the most reliable and effective protection against the vast majority of infectious diseases. Tick-borne encephalitis (TBE) represents a high-priority medical issue at the territory of the Eurasian continent. Of great importance is assessing a role of di...

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Autores principales:	G. N. Leonova, O. S. Majstrovskaya, V. A. Lubova, N. B. Sanina
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2019
Materias:	tick-borne encephalitis protective antibody titer dynamics of virus neutralization in vitro ex vivo in vivo Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/f2a5815719094119b5f44c795f01ed13
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spelling	oai:doaj.org-article:f2a5815719094119b5f44c795f01ed132021-11-22T07:09:53ZComprehensive assessment of specific antibodies on infectious activity of tick-borne encephalitis virus2220-76192313-739810.15789/2220-7619-2019-3-4-559-567https://doaj.org/article/f2a5815719094119b5f44c795f01ed132019-11-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/802https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Vaccines for prophylactic immunization provide the most reliable and effective protection against the vast majority of infectious diseases. Tick-borne encephalitis (TBE) represents a high-priority medical issue at the territory of the Eurasian continent. Of great importance is assessing a role of distinct antibody titers especially low titers, observed quite often in vaccinated individuals, sometimes posing obstacles in determining a threshold of seropositivity as well as the level of specific protection against TBE virus. We aimed at obtaining data to assess antiviral activity of virus-specific antibodies with distinct titer levels based on the in vitro, ex vivo and in vivo experimental studies with a highly virulent Far-Eastern strain of tick-borne encephalitis virus. The in vitro, ex vivo and in vivo comprehensive experimental studies with a highly virulent Far-Eastern strain of tick-borne encephalitis virus (TBEV) were conducted and the dynamics of antiviral activity of virus-specific antibodies at variable titers (1:100–1:3200) was measured (timeframe ranged within 1–96 hours p.i.) to provide a rationale for evaluating the antiviral immune response. It was found that the in vitro experiments demonstrated that the IgG at 1:100 titer exerted a weak anti-TBEV neutralizing effect at all time-points examined. The IgG 1:400 titer caused a 2 log PFU/mL decline in TBEV Dal strain yield at 72 h post-infection, whereas at 1:3200 titer it completely suppressed TBEV replication throughout the observation period. The ex vivo experiments with blood serum obtained from vaccinated subjects demonstrating a range of TBEV antibody titers (sera from vaccinated individuals with varying anti-TBEV antibody titers) and in vivo (outinbred white mice) experiments revealed a delayed virus elimination for antibody titers at 1:100 and 1:200 as well as rapid virus elimination (1–2 days p.i.) for antibody titers greater than 1:400. Thus, antibody titer at 1:400 may be considered as the universal anti-TBEV protection threshold. In order to properly conclude regarding the revaccination schedule it is advised to start with testing blood serum for durability of anti-TBEV immune response. Subjects with TBEV antibody titers at 1:100 and 1:200 should be strongly recommended to undergo a mandatory revaccination. Such an approach is believed to be the most effective way toward enhancing efficacy of vaccine-mediated protection against TBE.G. N. LeonovaO. S. MajstrovskayaV. A. LubovaN. B. SaninaSankt-Peterburg : NIIÈM imeni Pasteraarticletick-borne encephalitisprotective antibody titerdynamics of virus neutralization in vitroex vivoin vivoInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 9, Iss 3-4, Pp 559-567 (2019)
institution	DOAJ
collection	DOAJ
language	RU
topic	tick-borne encephalitis protective antibody titer dynamics of virus neutralization in vitro ex vivo in vivo Infectious and parasitic diseases RC109-216
spellingShingle	tick-borne encephalitis protective antibody titer dynamics of virus neutralization in vitro ex vivo in vivo Infectious and parasitic diseases RC109-216 G. N. Leonova O. S. Majstrovskaya V. A. Lubova N. B. Sanina Comprehensive assessment of specific antibodies on infectious activity of tick-borne encephalitis virus
description	Vaccines for prophylactic immunization provide the most reliable and effective protection against the vast majority of infectious diseases. Tick-borne encephalitis (TBE) represents a high-priority medical issue at the territory of the Eurasian continent. Of great importance is assessing a role of distinct antibody titers especially low titers, observed quite often in vaccinated individuals, sometimes posing obstacles in determining a threshold of seropositivity as well as the level of specific protection against TBE virus. We aimed at obtaining data to assess antiviral activity of virus-specific antibodies with distinct titer levels based on the in vitro, ex vivo and in vivo experimental studies with a highly virulent Far-Eastern strain of tick-borne encephalitis virus. The in vitro, ex vivo and in vivo comprehensive experimental studies with a highly virulent Far-Eastern strain of tick-borne encephalitis virus (TBEV) were conducted and the dynamics of antiviral activity of virus-specific antibodies at variable titers (1:100–1:3200) was measured (timeframe ranged within 1–96 hours p.i.) to provide a rationale for evaluating the antiviral immune response. It was found that the in vitro experiments demonstrated that the IgG at 1:100 titer exerted a weak anti-TBEV neutralizing effect at all time-points examined. The IgG 1:400 titer caused a 2 log PFU/mL decline in TBEV Dal strain yield at 72 h post-infection, whereas at 1:3200 titer it completely suppressed TBEV replication throughout the observation period. The ex vivo experiments with blood serum obtained from vaccinated subjects demonstrating a range of TBEV antibody titers (sera from vaccinated individuals with varying anti-TBEV antibody titers) and in vivo (outinbred white mice) experiments revealed a delayed virus elimination for antibody titers at 1:100 and 1:200 as well as rapid virus elimination (1–2 days p.i.) for antibody titers greater than 1:400. Thus, antibody titer at 1:400 may be considered as the universal anti-TBEV protection threshold. In order to properly conclude regarding the revaccination schedule it is advised to start with testing blood serum for durability of anti-TBEV immune response. Subjects with TBEV antibody titers at 1:100 and 1:200 should be strongly recommended to undergo a mandatory revaccination. Such an approach is believed to be the most effective way toward enhancing efficacy of vaccine-mediated protection against TBE.
format	article
author	G. N. Leonova O. S. Majstrovskaya V. A. Lubova N. B. Sanina
author_facet	G. N. Leonova O. S. Majstrovskaya V. A. Lubova N. B. Sanina
author_sort	G. N. Leonova
title	Comprehensive assessment of specific antibodies on infectious activity of tick-borne encephalitis virus
title_short	Comprehensive assessment of specific antibodies on infectious activity of tick-borne encephalitis virus
title_full	Comprehensive assessment of specific antibodies on infectious activity of tick-borne encephalitis virus
title_fullStr	Comprehensive assessment of specific antibodies on infectious activity of tick-borne encephalitis virus
title_full_unstemmed	Comprehensive assessment of specific antibodies on infectious activity of tick-borne encephalitis virus
title_sort	comprehensive assessment of specific antibodies on infectious activity of tick-borne encephalitis virus
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2019
url	https://doaj.org/article/f2a5815719094119b5f44c795f01ed13
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Comprehensive assessment of specific antibodies on infectious activity of tick-borne encephalitis virus

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