Effect of Light and p-Coumaric Acid on the Growth and Expression of Genes Related to Oxidative Stress in Brettanomyces bruxellensis LAMAP2480

Brettanomyces bruxellensis is considered the most significant contaminant yeast in the wine industry since it causes a deterioration in the organoleptic properties of the wine and significant economic losses. This deterioration is due to the production of volatile phenols from hydroxycinnamic acids....

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Autores principales:	Daniela Catrileo, Sandra Moreira, María Angélica Ganga, Liliana Godoy
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	B. bruxellensis light intensity oxidative stress ROS p-coumaric acid Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/f2a5e521fc15469fb6b9740545175efc
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spelling	oai:doaj.org-article:f2a5e521fc15469fb6b9740545175efc2021-12-01T01:45:28ZEffect of Light and p-Coumaric Acid on the Growth and Expression of Genes Related to Oxidative Stress in Brettanomyces bruxellensis LAMAP24801664-302X10.3389/fmicb.2021.747868https://doaj.org/article/f2a5e521fc15469fb6b9740545175efc2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.747868/fullhttps://doaj.org/toc/1664-302XBrettanomyces bruxellensis is considered the most significant contaminant yeast in the wine industry since it causes a deterioration in the organoleptic properties of the wine and significant economic losses. This deterioration is due to the production of volatile phenols from hydroxycinnamic acids. These compounds possess antimicrobial properties; however, B. bruxellensis can resist this effect because it metabolizes them into less toxic ones. Recent studies have reported that B. bruxellensis grows under different stress conditions, including p-coumaric acid (pCA) but effective methods for its control have not been found yet. Since that in other yeasts, such as Saccharomyces cerevisiae, it has been described that light affects its growth, and we evaluated whether the light would have a similar effect on B. bruxellensis. The results show that at light intensities of 2,500 and 4,000 lux in the absence of pCA, B. bruxellensis LAMAP2480 does not grow in the culture medium; however, when the medium contains this acid, the yeast adapts to both factors of stress managing to grow. The expression of genes related to oxidative stress in B. bruxellensis LAMAP2480, such as SOD1, GCN4, and ESBP6, showed a higher relative expression when the yeast was exposed to 2,500 lux compared to 4,000 lux, agreeing with the growth curves. This suggests that a higher expression of the genes studied would be related to stress-protective effects by pCA.Daniela CatrileoSandra MoreiraMaría Angélica GangaLiliana GodoyFrontiers Media S.A.articleB. bruxellensislight intensityoxidative stressROSp-coumaric acidMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	B. bruxellensis light intensity oxidative stress ROS p-coumaric acid Microbiology QR1-502
spellingShingle	B. bruxellensis light intensity oxidative stress ROS p-coumaric acid Microbiology QR1-502 Daniela Catrileo Sandra Moreira María Angélica Ganga Liliana Godoy Effect of Light and p-Coumaric Acid on the Growth and Expression of Genes Related to Oxidative Stress in Brettanomyces bruxellensis LAMAP2480
description	Brettanomyces bruxellensis is considered the most significant contaminant yeast in the wine industry since it causes a deterioration in the organoleptic properties of the wine and significant economic losses. This deterioration is due to the production of volatile phenols from hydroxycinnamic acids. These compounds possess antimicrobial properties; however, B. bruxellensis can resist this effect because it metabolizes them into less toxic ones. Recent studies have reported that B. bruxellensis grows under different stress conditions, including p-coumaric acid (pCA) but effective methods for its control have not been found yet. Since that in other yeasts, such as Saccharomyces cerevisiae, it has been described that light affects its growth, and we evaluated whether the light would have a similar effect on B. bruxellensis. The results show that at light intensities of 2,500 and 4,000 lux in the absence of pCA, B. bruxellensis LAMAP2480 does not grow in the culture medium; however, when the medium contains this acid, the yeast adapts to both factors of stress managing to grow. The expression of genes related to oxidative stress in B. bruxellensis LAMAP2480, such as SOD1, GCN4, and ESBP6, showed a higher relative expression when the yeast was exposed to 2,500 lux compared to 4,000 lux, agreeing with the growth curves. This suggests that a higher expression of the genes studied would be related to stress-protective effects by pCA.
format	article
author	Daniela Catrileo Sandra Moreira María Angélica Ganga Liliana Godoy
author_facet	Daniela Catrileo Sandra Moreira María Angélica Ganga Liliana Godoy
author_sort	Daniela Catrileo
title	Effect of Light and p-Coumaric Acid on the Growth and Expression of Genes Related to Oxidative Stress in Brettanomyces bruxellensis LAMAP2480
title_short	Effect of Light and p-Coumaric Acid on the Growth and Expression of Genes Related to Oxidative Stress in Brettanomyces bruxellensis LAMAP2480
title_full	Effect of Light and p-Coumaric Acid on the Growth and Expression of Genes Related to Oxidative Stress in Brettanomyces bruxellensis LAMAP2480
title_fullStr	Effect of Light and p-Coumaric Acid on the Growth and Expression of Genes Related to Oxidative Stress in Brettanomyces bruxellensis LAMAP2480
title_full_unstemmed	Effect of Light and p-Coumaric Acid on the Growth and Expression of Genes Related to Oxidative Stress in Brettanomyces bruxellensis LAMAP2480
title_sort	effect of light and p-coumaric acid on the growth and expression of genes related to oxidative stress in brettanomyces bruxellensis lamap2480
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/f2a5e521fc15469fb6b9740545175efc
work_keys_str_mv	AT danielacatrileo effectoflightandpcoumaricacidonthegrowthandexpressionofgenesrelatedtooxidativestressinbrettanomycesbruxellensislamap2480 AT sandramoreira effectoflightandpcoumaricacidonthegrowthandexpressionofgenesrelatedtooxidativestressinbrettanomycesbruxellensislamap2480 AT mariaangelicaganga effectoflightandpcoumaricacidonthegrowthandexpressionofgenesrelatedtooxidativestressinbrettanomycesbruxellensislamap2480 AT lilianagodoy effectoflightandpcoumaricacidonthegrowthandexpressionofgenesrelatedtooxidativestressinbrettanomycesbruxellensislamap2480
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Effect of Light and p-Coumaric Acid on the Growth and Expression of Genes Related to Oxidative Stress in Brettanomyces bruxellensis LAMAP2480

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