Field-friendly serological tests for determination of M. leprae-specific antibodies

Abstract Early detection of leprosy is key to reduce the ongoing transmission. Antibodies directed against M. leprae PGL-I represent a useful biomarker for detecting multibacillary (MB) patients. Since efficient leprosy diagnosis requires field-friendly test conditions, we evaluated two rapid latera...

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Autores principales:	Anouk van Hooij, Elisa M. Tjon Kon Fat, Susan J. F. van den Eeden, Louis Wilson, Moises Batista da Silva, Claudio G. Salgado, John S. Spencer, Paul L. A. M. Corstjens, Annemieke Geluk
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f2a8a590d48c4ec883a2189ae5b3d5ef
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spelling	oai:doaj.org-article:f2a8a590d48c4ec883a2189ae5b3d5ef2021-12-02T15:18:52ZField-friendly serological tests for determination of M. leprae-specific antibodies10.1038/s41598-017-07803-72045-2322https://doaj.org/article/f2a8a590d48c4ec883a2189ae5b3d5ef2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07803-7https://doaj.org/toc/2045-2322Abstract Early detection of leprosy is key to reduce the ongoing transmission. Antibodies directed against M. leprae PGL-I represent a useful biomarker for detecting multibacillary (MB) patients. Since efficient leprosy diagnosis requires field-friendly test conditions, we evaluated two rapid lateral flow assays (LFA) for detection of Mycobacterium leprae-specific antibodies: the visual immunogold OnSite Leprosy Ab Rapid test [Gold-LFA] and the quantitative, luminescent up-converting phosphor anti-PGL-I test [UCP-LFA]. Test performance was assessed in independent cohorts originating from three endemic areas. In the Philippine cohort comprising patients with high bacillary indices (BI; average:4,9), 94%(n = 161) of MB patients were identified by UCP-LFA and 78%(n = 133) by Gold-LFA. In the Bangladeshi cohort, including mainly MB patients with low BI (average:1), 41%(n = 14) and 44%(n = 15) were detected by UCP-LFA and Gold-LFA, respectively. In the third cohort of schoolchildren from a leprosy hyperendemic region in Brazil, both tests detected 28%(n = 17) seropositivity. Both rapid tests corresponded well with BI(p < 0.0001), with a fairly higher sensitivity obtained with the UCP-LFA assay. However, due to the spectral character of leprosy, additional, cellular biomarkers are required to detect patients with low BIs. Therefore, the UCP-LFA platform, which allows multiplexing with differential biomarkers, offers more cutting-edge potential for diagnosis across the whole leprosy spectrum.Anouk van HooijElisa M. Tjon Kon FatSusan J. F. van den EedenLouis WilsonMoises Batista da SilvaClaudio G. SalgadoJohn S. SpencerPaul L. A. M. CorstjensAnnemieke GelukNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anouk van Hooij Elisa M. Tjon Kon Fat Susan J. F. van den Eeden Louis Wilson Moises Batista da Silva Claudio G. Salgado John S. Spencer Paul L. A. M. Corstjens Annemieke Geluk Field-friendly serological tests for determination of M. leprae-specific antibodies
description	Abstract Early detection of leprosy is key to reduce the ongoing transmission. Antibodies directed against M. leprae PGL-I represent a useful biomarker for detecting multibacillary (MB) patients. Since efficient leprosy diagnosis requires field-friendly test conditions, we evaluated two rapid lateral flow assays (LFA) for detection of Mycobacterium leprae-specific antibodies: the visual immunogold OnSite Leprosy Ab Rapid test [Gold-LFA] and the quantitative, luminescent up-converting phosphor anti-PGL-I test [UCP-LFA]. Test performance was assessed in independent cohorts originating from three endemic areas. In the Philippine cohort comprising patients with high bacillary indices (BI; average:4,9), 94%(n = 161) of MB patients were identified by UCP-LFA and 78%(n = 133) by Gold-LFA. In the Bangladeshi cohort, including mainly MB patients with low BI (average:1), 41%(n = 14) and 44%(n = 15) were detected by UCP-LFA and Gold-LFA, respectively. In the third cohort of schoolchildren from a leprosy hyperendemic region in Brazil, both tests detected 28%(n = 17) seropositivity. Both rapid tests corresponded well with BI(p < 0.0001), with a fairly higher sensitivity obtained with the UCP-LFA assay. However, due to the spectral character of leprosy, additional, cellular biomarkers are required to detect patients with low BIs. Therefore, the UCP-LFA platform, which allows multiplexing with differential biomarkers, offers more cutting-edge potential for diagnosis across the whole leprosy spectrum.
format	article
author	Anouk van Hooij Elisa M. Tjon Kon Fat Susan J. F. van den Eeden Louis Wilson Moises Batista da Silva Claudio G. Salgado John S. Spencer Paul L. A. M. Corstjens Annemieke Geluk
author_facet	Anouk van Hooij Elisa M. Tjon Kon Fat Susan J. F. van den Eeden Louis Wilson Moises Batista da Silva Claudio G. Salgado John S. Spencer Paul L. A. M. Corstjens Annemieke Geluk
author_sort	Anouk van Hooij
title	Field-friendly serological tests for determination of M. leprae-specific antibodies
title_short	Field-friendly serological tests for determination of M. leprae-specific antibodies
title_full	Field-friendly serological tests for determination of M. leprae-specific antibodies
title_fullStr	Field-friendly serological tests for determination of M. leprae-specific antibodies
title_full_unstemmed	Field-friendly serological tests for determination of M. leprae-specific antibodies
title_sort	field-friendly serological tests for determination of m. leprae-specific antibodies
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f2a8a590d48c4ec883a2189ae5b3d5ef
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Field-friendly serological tests for determination of M. leprae-specific antibodies

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