Electric-field assisted 3D-fibrous bioceramic-based scaffolds for bone tissue regeneration: Fabrication, characterization, and in vitro cellular activities

Abstract Nano/microfibrous structure can induce high cellular activities because of the topological similarity of the extracellular matrix, and thus, are widely used in various tissue regenerative materials. However, the fabrication of a bioceramic (high weight percent)-based 3D microfibrous structu...

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Autores principales:	Minseong Kim, Hui-suk Yun, Geun Hyung Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f2aa0f085fe34f489b3e9ad6b9603a67
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spelling	oai:doaj.org-article:f2aa0f085fe34f489b3e9ad6b9603a672021-12-02T12:31:59ZElectric-field assisted 3D-fibrous bioceramic-based scaffolds for bone tissue regeneration: Fabrication, characterization, and in vitro cellular activities10.1038/s41598-017-03461-x2045-2322https://doaj.org/article/f2aa0f085fe34f489b3e9ad6b9603a672017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03461-xhttps://doaj.org/toc/2045-2322Abstract Nano/microfibrous structure can induce high cellular activities because of the topological similarity of the extracellular matrix, and thus, are widely used in various tissue regenerative materials. However, the fabrication of a bioceramic (high weight percent)-based 3D microfibrous structure is extremely difficult because of the low process-ability of bioceramics. In addition, three-dimensional (3D) microfibrous structure can induce more realistic cellular behavior when compared to that of 2D fibrous structure. Hence, the requirement of a 3D fibrous ceramic-based structure is an important issue in bioceramic scaffolds. In this study, a bioceramic (α-TCP)-based scaffold in which the weight fraction of the ceramic exceeded 70% was fabricated using an electrohydrodynamic printing (EHDP) process. The fabricated ceramic structure consisted of layer-by-layered struts entangled with polycaprolactone microfibers and the bioceramic phase. Various processing conditions (such as applied electric field, flow rate, nozzle size, and weight fraction of the bioceramic) were manipulated to obtain an optimal processing window. A 3D printed porous structure was used as a control, which had pore geometry similar to that of a structure fabricated using the EHDP process. Various physical and cellular activities using preosteoblasts (MC3T3-E1) helped confirm that the newly designed bioceramic scaffold demonstrated significantly high metabolic activity and mineralization.Minseong KimHui-suk YunGeun Hyung KimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Minseong Kim Hui-suk Yun Geun Hyung Kim Electric-field assisted 3D-fibrous bioceramic-based scaffolds for bone tissue regeneration: Fabrication, characterization, and in vitro cellular activities
description	Abstract Nano/microfibrous structure can induce high cellular activities because of the topological similarity of the extracellular matrix, and thus, are widely used in various tissue regenerative materials. However, the fabrication of a bioceramic (high weight percent)-based 3D microfibrous structure is extremely difficult because of the low process-ability of bioceramics. In addition, three-dimensional (3D) microfibrous structure can induce more realistic cellular behavior when compared to that of 2D fibrous structure. Hence, the requirement of a 3D fibrous ceramic-based structure is an important issue in bioceramic scaffolds. In this study, a bioceramic (α-TCP)-based scaffold in which the weight fraction of the ceramic exceeded 70% was fabricated using an electrohydrodynamic printing (EHDP) process. The fabricated ceramic structure consisted of layer-by-layered struts entangled with polycaprolactone microfibers and the bioceramic phase. Various processing conditions (such as applied electric field, flow rate, nozzle size, and weight fraction of the bioceramic) were manipulated to obtain an optimal processing window. A 3D printed porous structure was used as a control, which had pore geometry similar to that of a structure fabricated using the EHDP process. Various physical and cellular activities using preosteoblasts (MC3T3-E1) helped confirm that the newly designed bioceramic scaffold demonstrated significantly high metabolic activity and mineralization.
format	article
author	Minseong Kim Hui-suk Yun Geun Hyung Kim
author_facet	Minseong Kim Hui-suk Yun Geun Hyung Kim
author_sort	Minseong Kim
title	Electric-field assisted 3D-fibrous bioceramic-based scaffolds for bone tissue regeneration: Fabrication, characterization, and in vitro cellular activities
title_short	Electric-field assisted 3D-fibrous bioceramic-based scaffolds for bone tissue regeneration: Fabrication, characterization, and in vitro cellular activities
title_full	Electric-field assisted 3D-fibrous bioceramic-based scaffolds for bone tissue regeneration: Fabrication, characterization, and in vitro cellular activities
title_fullStr	Electric-field assisted 3D-fibrous bioceramic-based scaffolds for bone tissue regeneration: Fabrication, characterization, and in vitro cellular activities
title_full_unstemmed	Electric-field assisted 3D-fibrous bioceramic-based scaffolds for bone tissue regeneration: Fabrication, characterization, and in vitro cellular activities
title_sort	electric-field assisted 3d-fibrous bioceramic-based scaffolds for bone tissue regeneration: fabrication, characterization, and in vitro cellular activities
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f2aa0f085fe34f489b3e9ad6b9603a67
work_keys_str_mv	AT minseongkim electricfieldassisted3dfibrousbioceramicbasedscaffoldsforbonetissueregenerationfabricationcharacterizationandinvitrocellularactivities AT huisukyun electricfieldassisted3dfibrousbioceramicbasedscaffoldsforbonetissueregenerationfabricationcharacterizationandinvitrocellularactivities AT geunhyungkim electricfieldassisted3dfibrousbioceramicbasedscaffoldsforbonetissueregenerationfabricationcharacterizationandinvitrocellularactivities
_version_	1718394253380419584

Electric-field assisted 3D-fibrous bioceramic-based scaffolds for bone tissue regeneration: Fabrication, characterization, and in vitro cellular activities

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