Verteporfin inhibits growth of human glioma in vitro without light activation

Abstract Verteporfin (VP), a light-activated drug used in photodynamic therapy for the treatment of choroidal neovascular membranes, has also been shown to be an effective inhibitor of malignant cells. Recently, studies have demonstrated that, even without photo-activation, VP may still inhibit cert...

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Autores principales:	Ahmad Al-Moujahed, Katarzyna Brodowska, Tomasz P. Stryjewski, Nikolaos E. Efstathiou, Ioannis Vasilikos, Joanna Cichy, Joan W. Miller, Evangelos Gragoudas, Demetrios G. Vavvas
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f2b0e6444b7e4b96bbf25cb0fb97f1fa
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spelling	oai:doaj.org-article:f2b0e6444b7e4b96bbf25cb0fb97f1fa2021-12-02T12:32:43ZVerteporfin inhibits growth of human glioma in vitro without light activation10.1038/s41598-017-07632-82045-2322https://doaj.org/article/f2b0e6444b7e4b96bbf25cb0fb97f1fa2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07632-8https://doaj.org/toc/2045-2322Abstract Verteporfin (VP), a light-activated drug used in photodynamic therapy for the treatment of choroidal neovascular membranes, has also been shown to be an effective inhibitor of malignant cells. Recently, studies have demonstrated that, even without photo-activation, VP may still inhibit certain tumor cell lines, including ovarian cancer, hepatocarcinoma and retinoblastoma, through the inhibition of the YAP-TEAD complex. In this study, we examined the effects of VP without light activation on human glioma cell lines (LN229 and SNB19). Through western blot analysis, we identified that human glioma cells that were exposed to VP without light activation demonstrated a downregulation of YAP-TEAD-associated downstream signaling molecules, including c-myc, axl, CTGF, cyr61 and survivin and upregulation of the tumor growth inhibitor molecule p38 MAPK. In addition, we observed that expression of VEGFA and the pluripotent marker Oct-4 were also decreased. Verteporfin did not alter the Akt survival pathway or the mTor pathway but there was a modest increase in LC3-IIB, a marker of autophagosome biogenesis. This study suggests that verteporfin should be further explored as an adjuvant therapy for the treatment of glioblastoma.Ahmad Al-MoujahedKatarzyna BrodowskaTomasz P. StryjewskiNikolaos E. EfstathiouIoannis VasilikosJoanna CichyJoan W. MillerEvangelos GragoudasDemetrios G. VavvasNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ahmad Al-Moujahed Katarzyna Brodowska Tomasz P. Stryjewski Nikolaos E. Efstathiou Ioannis Vasilikos Joanna Cichy Joan W. Miller Evangelos Gragoudas Demetrios G. Vavvas Verteporfin inhibits growth of human glioma in vitro without light activation
description	Abstract Verteporfin (VP), a light-activated drug used in photodynamic therapy for the treatment of choroidal neovascular membranes, has also been shown to be an effective inhibitor of malignant cells. Recently, studies have demonstrated that, even without photo-activation, VP may still inhibit certain tumor cell lines, including ovarian cancer, hepatocarcinoma and retinoblastoma, through the inhibition of the YAP-TEAD complex. In this study, we examined the effects of VP without light activation on human glioma cell lines (LN229 and SNB19). Through western blot analysis, we identified that human glioma cells that were exposed to VP without light activation demonstrated a downregulation of YAP-TEAD-associated downstream signaling molecules, including c-myc, axl, CTGF, cyr61 and survivin and upregulation of the tumor growth inhibitor molecule p38 MAPK. In addition, we observed that expression of VEGFA and the pluripotent marker Oct-4 were also decreased. Verteporfin did not alter the Akt survival pathway or the mTor pathway but there was a modest increase in LC3-IIB, a marker of autophagosome biogenesis. This study suggests that verteporfin should be further explored as an adjuvant therapy for the treatment of glioblastoma.
format	article
author	Ahmad Al-Moujahed Katarzyna Brodowska Tomasz P. Stryjewski Nikolaos E. Efstathiou Ioannis Vasilikos Joanna Cichy Joan W. Miller Evangelos Gragoudas Demetrios G. Vavvas
author_facet	Ahmad Al-Moujahed Katarzyna Brodowska Tomasz P. Stryjewski Nikolaos E. Efstathiou Ioannis Vasilikos Joanna Cichy Joan W. Miller Evangelos Gragoudas Demetrios G. Vavvas
author_sort	Ahmad Al-Moujahed
title	Verteporfin inhibits growth of human glioma in vitro without light activation
title_short	Verteporfin inhibits growth of human glioma in vitro without light activation
title_full	Verteporfin inhibits growth of human glioma in vitro without light activation
title_fullStr	Verteporfin inhibits growth of human glioma in vitro without light activation
title_full_unstemmed	Verteporfin inhibits growth of human glioma in vitro without light activation
title_sort	verteporfin inhibits growth of human glioma in vitro without light activation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f2b0e6444b7e4b96bbf25cb0fb97f1fa
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Verteporfin inhibits growth of human glioma in vitro without light activation

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