The zebrafish as a new model for the in vivo study of Shigella flexneri interaction with phagocytes and bacterial autophagy.

Autophagy, an ancient and highly conserved intracellular degradation process, is viewed as a critical component of innate immunity because of its ability to deliver cytosolic bacteria to the lysosome. However, the role of bacterial autophagy in vivo remains poorly understood. The zebrafish (Danio re...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Serge Mostowy, Laurent Boucontet, Maria J Mazon Moya, Andrea Sirianni, Pierre Boudinot, Michael Hollinshead, Pascale Cossart, Philippe Herbomel, Jean-Pierre Levraud, Emma Colucci-Guyon
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f2b25e2ba579463bad05187a7f4dab33
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f2b25e2ba579463bad05187a7f4dab33
record_format	dspace
spelling	oai:doaj.org-article:f2b25e2ba579463bad05187a7f4dab332021-11-18T06:07:41ZThe zebrafish as a new model for the in vivo study of Shigella flexneri interaction with phagocytes and bacterial autophagy.1553-73661553-737410.1371/journal.ppat.1003588https://doaj.org/article/f2b25e2ba579463bad05187a7f4dab332013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24039575/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Autophagy, an ancient and highly conserved intracellular degradation process, is viewed as a critical component of innate immunity because of its ability to deliver cytosolic bacteria to the lysosome. However, the role of bacterial autophagy in vivo remains poorly understood. The zebrafish (Danio rerio) has emerged as a vertebrate model for the study of infections because it is optically accessible at the larval stages when the innate immune system is already functional. Here, we have characterized the susceptibility of zebrafish larvae to Shigella flexneri, a paradigm for bacterial autophagy, and have used this model to study Shigella-phagocyte interactions in vivo. Depending on the dose, S. flexneri injected in zebrafish larvae were either cleared in a few days or resulted in a progressive and ultimately fatal infection. Using high resolution live imaging, we found that S. flexneri were rapidly engulfed by macrophages and neutrophils; moreover we discovered a scavenger role for neutrophils in eliminating infected dead macrophages and non-immune cell types that failed to control Shigella infection. We observed that intracellular S. flexneri could escape to the cytosol, induce septin caging and be targeted to autophagy in vivo. Depletion of p62 (sequestosome 1 or SQSTM1), an adaptor protein critical for bacterial autophagy in vitro, significantly increased bacterial burden and host susceptibility to infection. These results show the zebrafish larva as a new model for the study of S. flexneri interaction with phagocytes, and the manipulation of autophagy for anti-bacterial therapy in vivo.Serge MostowyLaurent BoucontetMaria J Mazon MoyaAndrea SirianniPierre BoudinotMichael HollinsheadPascale CossartPhilippe HerbomelJean-Pierre LevraudEmma Colucci-GuyonPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 9, Iss 9, p e1003588 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Serge Mostowy Laurent Boucontet Maria J Mazon Moya Andrea Sirianni Pierre Boudinot Michael Hollinshead Pascale Cossart Philippe Herbomel Jean-Pierre Levraud Emma Colucci-Guyon The zebrafish as a new model for the in vivo study of Shigella flexneri interaction with phagocytes and bacterial autophagy.
description	Autophagy, an ancient and highly conserved intracellular degradation process, is viewed as a critical component of innate immunity because of its ability to deliver cytosolic bacteria to the lysosome. However, the role of bacterial autophagy in vivo remains poorly understood. The zebrafish (Danio rerio) has emerged as a vertebrate model for the study of infections because it is optically accessible at the larval stages when the innate immune system is already functional. Here, we have characterized the susceptibility of zebrafish larvae to Shigella flexneri, a paradigm for bacterial autophagy, and have used this model to study Shigella-phagocyte interactions in vivo. Depending on the dose, S. flexneri injected in zebrafish larvae were either cleared in a few days or resulted in a progressive and ultimately fatal infection. Using high resolution live imaging, we found that S. flexneri were rapidly engulfed by macrophages and neutrophils; moreover we discovered a scavenger role for neutrophils in eliminating infected dead macrophages and non-immune cell types that failed to control Shigella infection. We observed that intracellular S. flexneri could escape to the cytosol, induce septin caging and be targeted to autophagy in vivo. Depletion of p62 (sequestosome 1 or SQSTM1), an adaptor protein critical for bacterial autophagy in vitro, significantly increased bacterial burden and host susceptibility to infection. These results show the zebrafish larva as a new model for the study of S. flexneri interaction with phagocytes, and the manipulation of autophagy for anti-bacterial therapy in vivo.
format	article
author	Serge Mostowy Laurent Boucontet Maria J Mazon Moya Andrea Sirianni Pierre Boudinot Michael Hollinshead Pascale Cossart Philippe Herbomel Jean-Pierre Levraud Emma Colucci-Guyon
author_facet	Serge Mostowy Laurent Boucontet Maria J Mazon Moya Andrea Sirianni Pierre Boudinot Michael Hollinshead Pascale Cossart Philippe Herbomel Jean-Pierre Levraud Emma Colucci-Guyon
author_sort	Serge Mostowy
title	The zebrafish as a new model for the in vivo study of Shigella flexneri interaction with phagocytes and bacterial autophagy.
title_short	The zebrafish as a new model for the in vivo study of Shigella flexneri interaction with phagocytes and bacterial autophagy.
title_full	The zebrafish as a new model for the in vivo study of Shigella flexneri interaction with phagocytes and bacterial autophagy.
title_fullStr	The zebrafish as a new model for the in vivo study of Shigella flexneri interaction with phagocytes and bacterial autophagy.
title_full_unstemmed	The zebrafish as a new model for the in vivo study of Shigella flexneri interaction with phagocytes and bacterial autophagy.
title_sort	zebrafish as a new model for the in vivo study of shigella flexneri interaction with phagocytes and bacterial autophagy.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/f2b25e2ba579463bad05187a7f4dab33
work_keys_str_mv	AT sergemostowy thezebrafishasanewmodelfortheinvivostudyofshigellaflexneriinteractionwithphagocytesandbacterialautophagy AT laurentboucontet thezebrafishasanewmodelfortheinvivostudyofshigellaflexneriinteractionwithphagocytesandbacterialautophagy AT mariajmazonmoya thezebrafishasanewmodelfortheinvivostudyofshigellaflexneriinteractionwithphagocytesandbacterialautophagy AT andreasirianni thezebrafishasanewmodelfortheinvivostudyofshigellaflexneriinteractionwithphagocytesandbacterialautophagy AT pierreboudinot thezebrafishasanewmodelfortheinvivostudyofshigellaflexneriinteractionwithphagocytesandbacterialautophagy AT michaelhollinshead thezebrafishasanewmodelfortheinvivostudyofshigellaflexneriinteractionwithphagocytesandbacterialautophagy AT pascalecossart thezebrafishasanewmodelfortheinvivostudyofshigellaflexneriinteractionwithphagocytesandbacterialautophagy AT philippeherbomel thezebrafishasanewmodelfortheinvivostudyofshigellaflexneriinteractionwithphagocytesandbacterialautophagy AT jeanpierrelevraud thezebrafishasanewmodelfortheinvivostudyofshigellaflexneriinteractionwithphagocytesandbacterialautophagy AT emmacolucciguyon thezebrafishasanewmodelfortheinvivostudyofshigellaflexneriinteractionwithphagocytesandbacterialautophagy AT sergemostowy zebrafishasanewmodelfortheinvivostudyofshigellaflexneriinteractionwithphagocytesandbacterialautophagy AT laurentboucontet zebrafishasanewmodelfortheinvivostudyofshigellaflexneriinteractionwithphagocytesandbacterialautophagy AT mariajmazonmoya zebrafishasanewmodelfortheinvivostudyofshigellaflexneriinteractionwithphagocytesandbacterialautophagy AT andreasirianni zebrafishasanewmodelfortheinvivostudyofshigellaflexneriinteractionwithphagocytesandbacterialautophagy AT pierreboudinot zebrafishasanewmodelfortheinvivostudyofshigellaflexneriinteractionwithphagocytesandbacterialautophagy AT michaelhollinshead zebrafishasanewmodelfortheinvivostudyofshigellaflexneriinteractionwithphagocytesandbacterialautophagy AT pascalecossart zebrafishasanewmodelfortheinvivostudyofshigellaflexneriinteractionwithphagocytesandbacterialautophagy AT philippeherbomel zebrafishasanewmodelfortheinvivostudyofshigellaflexneriinteractionwithphagocytesandbacterialautophagy AT jeanpierrelevraud zebrafishasanewmodelfortheinvivostudyofshigellaflexneriinteractionwithphagocytesandbacterialautophagy AT emmacolucciguyon zebrafishasanewmodelfortheinvivostudyofshigellaflexneriinteractionwithphagocytesandbacterialautophagy
_version_	1718424577525153792

The zebrafish as a new model for the in vivo study of Shigella flexneri interaction with phagocytes and bacterial autophagy.

Ejemplares similares