Assembly of Schizosaccharomyces cryophilus chromosomes and their comparative genomic analyses revealed principles of genome evolution of the haploid fission yeasts

Abstract The fission yeast clade, which has a distinct life history from other yeasts, can provide important clues about evolutionary changes. To reveal these changes the large S. cryophilus supercontigs were assembled into chromosomes using synteny relationships and the conserved pericentromeric, s...

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Autores principales:	Lajos Ács-Szabó, László Attila Papp, Zsuzsa Antunovics, Matthias Sipiczki, Ida Miklós
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Octospori Subtelomeric Genes rDNA Arrays Distinct Life History Synteny Relationships Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f2b4a1d21d06442099a7c95dff7e4a88
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spelling	oai:doaj.org-article:f2b4a1d21d06442099a7c95dff7e4a882021-12-02T15:07:46ZAssembly of Schizosaccharomyces cryophilus chromosomes and their comparative genomic analyses revealed principles of genome evolution of the haploid fission yeasts10.1038/s41598-018-32525-92045-2322https://doaj.org/article/f2b4a1d21d06442099a7c95dff7e4a882018-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-32525-9https://doaj.org/toc/2045-2322Abstract The fission yeast clade, which has a distinct life history from other yeasts, can provide important clues about evolutionary changes. To reveal these changes the large S. cryophilus supercontigs were assembled into chromosomes using synteny relationships and the conserved pericentromeric, subtelomeric genes. Togetherness of the supercontigs was confirmed by PCR. Investigation of the gene order revealed localisation of the rDNA arrays, more than 300 new conserved orthologues and proved that S. cryophilus supercontigs were mosaics of collinear blocks. PFGE analysis showed that size of the S. cryophilus chromosomes differ from the S. pombe chromosomes. Comparative genomic analyses of the newly assembled chromosomes confirmed that the closest relative of S. cryophilus was S. octosporus not just in sequence similarity but also in a structural way, and revealed that preservation of the conserved regions did not arise from the lower number of chromosomal rearrangements. Translocations were more typical in the closely related species, while the number of inversions increased with the phylogenetic distances. Our data suggested that sites of the chromosomal rearrangements were not random and often associated with repetitive sequences, structural- and nucleotide evolution might correlate. Chromosomal rearrangements of the fission yeasts compared to other lineages were also discussed.Lajos Ács-SzabóLászló Attila PappZsuzsa AntunovicsMatthias SipiczkiIda MiklósNature PortfolioarticleOctosporiSubtelomeric GenesrDNA ArraysDistinct Life HistorySynteny RelationshipsMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Octospori Subtelomeric Genes rDNA Arrays Distinct Life History Synteny Relationships Medicine R Science Q
spellingShingle	Octospori Subtelomeric Genes rDNA Arrays Distinct Life History Synteny Relationships Medicine R Science Q Lajos Ács-Szabó László Attila Papp Zsuzsa Antunovics Matthias Sipiczki Ida Miklós Assembly of Schizosaccharomyces cryophilus chromosomes and their comparative genomic analyses revealed principles of genome evolution of the haploid fission yeasts
description	Abstract The fission yeast clade, which has a distinct life history from other yeasts, can provide important clues about evolutionary changes. To reveal these changes the large S. cryophilus supercontigs were assembled into chromosomes using synteny relationships and the conserved pericentromeric, subtelomeric genes. Togetherness of the supercontigs was confirmed by PCR. Investigation of the gene order revealed localisation of the rDNA arrays, more than 300 new conserved orthologues and proved that S. cryophilus supercontigs were mosaics of collinear blocks. PFGE analysis showed that size of the S. cryophilus chromosomes differ from the S. pombe chromosomes. Comparative genomic analyses of the newly assembled chromosomes confirmed that the closest relative of S. cryophilus was S. octosporus not just in sequence similarity but also in a structural way, and revealed that preservation of the conserved regions did not arise from the lower number of chromosomal rearrangements. Translocations were more typical in the closely related species, while the number of inversions increased with the phylogenetic distances. Our data suggested that sites of the chromosomal rearrangements were not random and often associated with repetitive sequences, structural- and nucleotide evolution might correlate. Chromosomal rearrangements of the fission yeasts compared to other lineages were also discussed.
format	article
author	Lajos Ács-Szabó László Attila Papp Zsuzsa Antunovics Matthias Sipiczki Ida Miklós
author_facet	Lajos Ács-Szabó László Attila Papp Zsuzsa Antunovics Matthias Sipiczki Ida Miklós
author_sort	Lajos Ács-Szabó
title	Assembly of Schizosaccharomyces cryophilus chromosomes and their comparative genomic analyses revealed principles of genome evolution of the haploid fission yeasts
title_short	Assembly of Schizosaccharomyces cryophilus chromosomes and their comparative genomic analyses revealed principles of genome evolution of the haploid fission yeasts
title_full	Assembly of Schizosaccharomyces cryophilus chromosomes and their comparative genomic analyses revealed principles of genome evolution of the haploid fission yeasts
title_fullStr	Assembly of Schizosaccharomyces cryophilus chromosomes and their comparative genomic analyses revealed principles of genome evolution of the haploid fission yeasts
title_full_unstemmed	Assembly of Schizosaccharomyces cryophilus chromosomes and their comparative genomic analyses revealed principles of genome evolution of the haploid fission yeasts
title_sort	assembly of schizosaccharomyces cryophilus chromosomes and their comparative genomic analyses revealed principles of genome evolution of the haploid fission yeasts
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/f2b4a1d21d06442099a7c95dff7e4a88
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Assembly of Schizosaccharomyces cryophilus chromosomes and their comparative genomic analyses revealed principles of genome evolution of the haploid fission yeasts

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