Transgene-free Genome Editing in Plants

Genome editing is widely used across plant species to generate and study the impact of functional mutations in crop improvement. However, transgene integration in plant genomes raises important legislative concerns regarding genetically modified organisms. Several strategies have been developed to r...

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Autores principales:	Xiaoyong Gu, Lijing Liu, Huawei Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	genome editing CRISPR/Cas9 transgene-free transgene integration editor delivery Biotechnology TP248.13-248.65 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/f2bdb101f5b34330842f02761cde7255
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id	oai:doaj.org-article:f2bdb101f5b34330842f02761cde7255
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spelling	oai:doaj.org-article:f2bdb101f5b34330842f02761cde72552021-12-02T11:43:01ZTransgene-free Genome Editing in Plants2673-343910.3389/fgeed.2021.805317https://doaj.org/article/f2bdb101f5b34330842f02761cde72552021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fgeed.2021.805317/fullhttps://doaj.org/toc/2673-3439Genome editing is widely used across plant species to generate and study the impact of functional mutations in crop improvement. However, transgene integration in plant genomes raises important legislative concerns regarding genetically modified organisms. Several strategies have been developed to remove or prevent the integration of gene editor constructs, which can be divided into three major categories: 1) elimination of transgenic sequences via genetic segregation; 2) transient editor expression from DNA vectors; and 3) DNA-independent editor delivery, including RNA or preassembled Cas9 protein-gRNA ribonucleoproteins (RNPs). Here, we summarize the main strategies employed to date and discuss the advantages and disadvantages of using these different tools. We hope that our work can provide important information concerning the value of alternative genome editing strategies to advance crop breeding.Xiaoyong GuLijing LiuHuawei ZhangFrontiers Media S.A.articlegenome editingCRISPR/Cas9transgene-freetransgene integrationeditor deliveryBiotechnologyTP248.13-248.65GeneticsQH426-470ENFrontiers in Genome Editing, Vol 3 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	genome editing CRISPR/Cas9 transgene-free transgene integration editor delivery Biotechnology TP248.13-248.65 Genetics QH426-470
spellingShingle	genome editing CRISPR/Cas9 transgene-free transgene integration editor delivery Biotechnology TP248.13-248.65 Genetics QH426-470 Xiaoyong Gu Lijing Liu Huawei Zhang Transgene-free Genome Editing in Plants
description	Genome editing is widely used across plant species to generate and study the impact of functional mutations in crop improvement. However, transgene integration in plant genomes raises important legislative concerns regarding genetically modified organisms. Several strategies have been developed to remove or prevent the integration of gene editor constructs, which can be divided into three major categories: 1) elimination of transgenic sequences via genetic segregation; 2) transient editor expression from DNA vectors; and 3) DNA-independent editor delivery, including RNA or preassembled Cas9 protein-gRNA ribonucleoproteins (RNPs). Here, we summarize the main strategies employed to date and discuss the advantages and disadvantages of using these different tools. We hope that our work can provide important information concerning the value of alternative genome editing strategies to advance crop breeding.
format	article
author	Xiaoyong Gu Lijing Liu Huawei Zhang
author_facet	Xiaoyong Gu Lijing Liu Huawei Zhang
author_sort	Xiaoyong Gu
title	Transgene-free Genome Editing in Plants
title_short	Transgene-free Genome Editing in Plants
title_full	Transgene-free Genome Editing in Plants
title_fullStr	Transgene-free Genome Editing in Plants
title_full_unstemmed	Transgene-free Genome Editing in Plants
title_sort	transgene-free genome editing in plants
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/f2bdb101f5b34330842f02761cde7255
work_keys_str_mv	AT xiaoyonggu transgenefreegenomeeditinginplants AT lijingliu transgenefreegenomeeditinginplants AT huaweizhang transgenefreegenomeeditinginplants
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Transgene-free Genome Editing in Plants

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