Co-infections observed in SARS-CoV-2 positive patients using a rapid diagnostic test

Abstract Rapid diagnostic tests are tools of paramount impact both for improving patient care and in antimicrobial management programs. Particularly in the case of respiratory infections, it is of great importance to quickly confirm/exclude the involvement of pathogens, be they bacteria or viruses,...

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Autores principales:	Carla Fontana, Marco Favaro, Silvia Minelli, Maria Cristina Bossa, Anna Altieri
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f2cd9b3246054c159a8889d7e35e2b7c
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spelling	oai:doaj.org-article:f2cd9b3246054c159a8889d7e35e2b7c2021-12-02T15:07:54ZCo-infections observed in SARS-CoV-2 positive patients using a rapid diagnostic test10.1038/s41598-021-95772-32045-2322https://doaj.org/article/f2cd9b3246054c159a8889d7e35e2b7c2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95772-3https://doaj.org/toc/2045-2322Abstract Rapid diagnostic tests are tools of paramount impact both for improving patient care and in antimicrobial management programs. Particularly in the case of respiratory infections, it is of great importance to quickly confirm/exclude the involvement of pathogens, be they bacteria or viruses, while obtaining information about the presence/absence of a genetic target of resistance to modulate antibiotic therapy. In this paper, we present our experiences with the use of the Biofire® FilmArray® Pneumonia Panel Plus (FAPP; bioMérieux; Marcy l’Etoile, France) to assess coinfection in COVID-19 patients. A total of 152 respiratory samples from consecutive patients were examined, and 93 (61%) were found to be FAPP positive, with the detection of bacteria and/or viruses. The patients were 93 males and 59 females with an average age of 65 years who were admitted to our hospital due to moderate/severe acute respiratory symptoms. Among the positive samples were 52 from sputum (SPU) and 41 from bronchoalveolar lavage (BAL). The most representative species was S. aureus (most isolates were mecA positive; 30/44, 62%), followed by gram-negative pathogens such as P. aeruginosa, K. pneumoniae, and A. baumannii. Evidence of a virus was rare. Cultures performed from BAL and SPU samples gave poor results. Most of the discrepant negative cultures were those in which FAPP detected pathogens with a microbial count ≤ 105 CFU/mL. H. influenzae was one of the most common pathogens lost by the conventional method. Despite the potential limitations of FAPP, which detects a defined number of pathogens, its advantages of rapid detection combined with predictive information regarding the antimicrobial resistance of pathogens through the detection of some relevant markers of resistance could be very useful for establishing empirical targeted therapy for the treatment of patients with respiratory failure. In the COVID era, we understand the importance of using antibiotics wisely to curb the phenomenon of antibiotic resistance.Carla FontanaMarco FavaroSilvia MinelliMaria Cristina BossaAnna AltieriNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Carla Fontana Marco Favaro Silvia Minelli Maria Cristina Bossa Anna Altieri Co-infections observed in SARS-CoV-2 positive patients using a rapid diagnostic test
description	Abstract Rapid diagnostic tests are tools of paramount impact both for improving patient care and in antimicrobial management programs. Particularly in the case of respiratory infections, it is of great importance to quickly confirm/exclude the involvement of pathogens, be they bacteria or viruses, while obtaining information about the presence/absence of a genetic target of resistance to modulate antibiotic therapy. In this paper, we present our experiences with the use of the Biofire® FilmArray® Pneumonia Panel Plus (FAPP; bioMérieux; Marcy l’Etoile, France) to assess coinfection in COVID-19 patients. A total of 152 respiratory samples from consecutive patients were examined, and 93 (61%) were found to be FAPP positive, with the detection of bacteria and/or viruses. The patients were 93 males and 59 females with an average age of 65 years who were admitted to our hospital due to moderate/severe acute respiratory symptoms. Among the positive samples were 52 from sputum (SPU) and 41 from bronchoalveolar lavage (BAL). The most representative species was S. aureus (most isolates were mecA positive; 30/44, 62%), followed by gram-negative pathogens such as P. aeruginosa, K. pneumoniae, and A. baumannii. Evidence of a virus was rare. Cultures performed from BAL and SPU samples gave poor results. Most of the discrepant negative cultures were those in which FAPP detected pathogens with a microbial count ≤ 105 CFU/mL. H. influenzae was one of the most common pathogens lost by the conventional method. Despite the potential limitations of FAPP, which detects a defined number of pathogens, its advantages of rapid detection combined with predictive information regarding the antimicrobial resistance of pathogens through the detection of some relevant markers of resistance could be very useful for establishing empirical targeted therapy for the treatment of patients with respiratory failure. In the COVID era, we understand the importance of using antibiotics wisely to curb the phenomenon of antibiotic resistance.
format	article
author	Carla Fontana Marco Favaro Silvia Minelli Maria Cristina Bossa Anna Altieri
author_facet	Carla Fontana Marco Favaro Silvia Minelli Maria Cristina Bossa Anna Altieri
author_sort	Carla Fontana
title	Co-infections observed in SARS-CoV-2 positive patients using a rapid diagnostic test
title_short	Co-infections observed in SARS-CoV-2 positive patients using a rapid diagnostic test
title_full	Co-infections observed in SARS-CoV-2 positive patients using a rapid diagnostic test
title_fullStr	Co-infections observed in SARS-CoV-2 positive patients using a rapid diagnostic test
title_full_unstemmed	Co-infections observed in SARS-CoV-2 positive patients using a rapid diagnostic test
title_sort	co-infections observed in sars-cov-2 positive patients using a rapid diagnostic test
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f2cd9b3246054c159a8889d7e35e2b7c
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Co-infections observed in SARS-CoV-2 positive patients using a rapid diagnostic test

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