JMJD3 and NF-κB-dependent activation of Notch1 gene is required for keratinocyte migration during skin wound healing

Abstract It has been shown that epigenetic regulation plays an important role in skin wound healing. We previously found that histone H3K27me3 demethylase JMJD3 regulates inflammation and cell migration in keratinocyte wound healing. In this study, we identified Notch1 as a direct target of JMJD3 an...

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Autores principales:	Jungtae Na, Jee Yoon Shin, Hayan Jeong, Jee Youn Lee, Beom Joon Kim, Won Sun Kim, Tae Young Yune, Bong-Gun Ju
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f2cde2a3be66455082ceaf75657918e5
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spelling	oai:doaj.org-article:f2cde2a3be66455082ceaf75657918e52021-12-02T12:31:48ZJMJD3 and NF-κB-dependent activation of Notch1 gene is required for keratinocyte migration during skin wound healing10.1038/s41598-017-06750-72045-2322https://doaj.org/article/f2cde2a3be66455082ceaf75657918e52017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06750-7https://doaj.org/toc/2045-2322Abstract It has been shown that epigenetic regulation plays an important role in skin wound healing. We previously found that histone H3K27me3 demethylase JMJD3 regulates inflammation and cell migration in keratinocyte wound healing. In this study, we identified Notch1 as a direct target of JMJD3 and NF-κB in wounded keratinocytes using in vitro cell and in vivo animal models. We found that Notch1 is up-regulated in the wound edge and its expression is dependent on JMJD3 and NF-κB in wounded keratinocytes. We also found that Notch1 activates the expression of RhoU and PLAU gene, which are critical regulators of cell migration. Consistently, depletion or inactivation of Notch1 resulted in decreased filopodia formation, increased focal adhesion and actin stress fiber, leading to reduced keratinocyte migration and skin wound healing. Thus, our findings provide the molecular mechanism involving JMJD3/NF-κB-Notch pathway in keratinocyte wound healing.Jungtae NaJee Yoon ShinHayan JeongJee Youn LeeBeom Joon KimWon Sun KimTae Young YuneBong-Gun JuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jungtae Na Jee Yoon Shin Hayan Jeong Jee Youn Lee Beom Joon Kim Won Sun Kim Tae Young Yune Bong-Gun Ju JMJD3 and NF-κB-dependent activation of Notch1 gene is required for keratinocyte migration during skin wound healing
description	Abstract It has been shown that epigenetic regulation plays an important role in skin wound healing. We previously found that histone H3K27me3 demethylase JMJD3 regulates inflammation and cell migration in keratinocyte wound healing. In this study, we identified Notch1 as a direct target of JMJD3 and NF-κB in wounded keratinocytes using in vitro cell and in vivo animal models. We found that Notch1 is up-regulated in the wound edge and its expression is dependent on JMJD3 and NF-κB in wounded keratinocytes. We also found that Notch1 activates the expression of RhoU and PLAU gene, which are critical regulators of cell migration. Consistently, depletion or inactivation of Notch1 resulted in decreased filopodia formation, increased focal adhesion and actin stress fiber, leading to reduced keratinocyte migration and skin wound healing. Thus, our findings provide the molecular mechanism involving JMJD3/NF-κB-Notch pathway in keratinocyte wound healing.
format	article
author	Jungtae Na Jee Yoon Shin Hayan Jeong Jee Youn Lee Beom Joon Kim Won Sun Kim Tae Young Yune Bong-Gun Ju
author_facet	Jungtae Na Jee Yoon Shin Hayan Jeong Jee Youn Lee Beom Joon Kim Won Sun Kim Tae Young Yune Bong-Gun Ju
author_sort	Jungtae Na
title	JMJD3 and NF-κB-dependent activation of Notch1 gene is required for keratinocyte migration during skin wound healing
title_short	JMJD3 and NF-κB-dependent activation of Notch1 gene is required for keratinocyte migration during skin wound healing
title_full	JMJD3 and NF-κB-dependent activation of Notch1 gene is required for keratinocyte migration during skin wound healing
title_fullStr	JMJD3 and NF-κB-dependent activation of Notch1 gene is required for keratinocyte migration during skin wound healing
title_full_unstemmed	JMJD3 and NF-κB-dependent activation of Notch1 gene is required for keratinocyte migration during skin wound healing
title_sort	jmjd3 and nf-κb-dependent activation of notch1 gene is required for keratinocyte migration during skin wound healing
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f2cde2a3be66455082ceaf75657918e5
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JMJD3 and NF-κB-dependent activation of Notch1 gene is required for keratinocyte migration during skin wound healing

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