Two-colour single-molecule photoinduced electron transfer fluorescence imaging microscopy of chaperone dynamics

Revealing mechanisms of complex protein machines requires simultaneous exploration of multiple structural coordinates. Here the authors report two-colour fluorescence microscopy combined with photoinduced electron transfer probes to simultaneously detect two structural coordinates in single protein...

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Autores principales: Jonathan Schubert, Andrea Schulze, Chrisostomos Prodromou, Hannes Neuweiler
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/f2d844cfcf424f0482c2c3ef43b998c7
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Sumario:Revealing mechanisms of complex protein machines requires simultaneous exploration of multiple structural coordinates. Here the authors report two-colour fluorescence microscopy combined with photoinduced electron transfer probes to simultaneously detect two structural coordinates in single protein molecules.