3D cell sheet structure augments mesenchymal stem cell cytokine production

Abstract Mesenchymal stem cells (MSCs) secrete paracrine factors that play crucial roles during tissue regeneration. An increasing body of evidence suggests that this paracrine function is enhanced by MSC cultivation in three-dimensional (3D) tissue-like microenvironments. Toward this end, this stud...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sophia Bou-Ghannam, Kyungsook Kim, David W. Grainger, Teruo Okano
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f2eaceb8ac6043cdb8dc2f9fddbe681f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f2eaceb8ac6043cdb8dc2f9fddbe681f
record_format	dspace
spelling	oai:doaj.org-article:f2eaceb8ac6043cdb8dc2f9fddbe681f2021-12-02T14:27:56Z3D cell sheet structure augments mesenchymal stem cell cytokine production10.1038/s41598-021-87571-72045-2322https://doaj.org/article/f2eaceb8ac6043cdb8dc2f9fddbe681f2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87571-7https://doaj.org/toc/2045-2322Abstract Mesenchymal stem cells (MSCs) secrete paracrine factors that play crucial roles during tissue regeneration. An increasing body of evidence suggests that this paracrine function is enhanced by MSC cultivation in three-dimensional (3D) tissue-like microenvironments. Toward this end, this study explored scaffold-free cell sheet technology as a new 3D platform. MSCs cultivated on temperature-responsive culture dishes to a confluent 2D monolayer were harvested by temperature reduction from 37 to 20 °C that induces a surface wettability transition from hydrophobic to hydrophilic. Release of culture-adherent tension induced spontaneous cell sheet contraction, reducing the diameter 2.4-fold, and increasing the thickness 8.0-fold to render a 3D tissue-like construct with a 36% increase in tissue volume. This 2D-to-3D transition reorganized MSC actin cytoskeleton from aligned to multidirectional, corresponding to a cell morphological change from elongated in 2D monolayers to rounded in 3D cell sheets. 3D culture increased MSC gene expression of cell interaction proteins, β-catenin, integrin β1, and connexin 43, and of pro-tissue regenerative cytokines, vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), and interleukin-10 (IL-10), and increased VEGF secretion per MSC 2.1-fold relative to 2D cultures. Together, these findings demonstrate that MSC therapeutic potency can be enhanced by 3D cell sheet tissue structure.Sophia Bou-GhannamKyungsook KimDavid W. GraingerTeruo OkanoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sophia Bou-Ghannam Kyungsook Kim David W. Grainger Teruo Okano 3D cell sheet structure augments mesenchymal stem cell cytokine production
description	Abstract Mesenchymal stem cells (MSCs) secrete paracrine factors that play crucial roles during tissue regeneration. An increasing body of evidence suggests that this paracrine function is enhanced by MSC cultivation in three-dimensional (3D) tissue-like microenvironments. Toward this end, this study explored scaffold-free cell sheet technology as a new 3D platform. MSCs cultivated on temperature-responsive culture dishes to a confluent 2D monolayer were harvested by temperature reduction from 37 to 20 °C that induces a surface wettability transition from hydrophobic to hydrophilic. Release of culture-adherent tension induced spontaneous cell sheet contraction, reducing the diameter 2.4-fold, and increasing the thickness 8.0-fold to render a 3D tissue-like construct with a 36% increase in tissue volume. This 2D-to-3D transition reorganized MSC actin cytoskeleton from aligned to multidirectional, corresponding to a cell morphological change from elongated in 2D monolayers to rounded in 3D cell sheets. 3D culture increased MSC gene expression of cell interaction proteins, β-catenin, integrin β1, and connexin 43, and of pro-tissue regenerative cytokines, vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), and interleukin-10 (IL-10), and increased VEGF secretion per MSC 2.1-fold relative to 2D cultures. Together, these findings demonstrate that MSC therapeutic potency can be enhanced by 3D cell sheet tissue structure.
format	article
author	Sophia Bou-Ghannam Kyungsook Kim David W. Grainger Teruo Okano
author_facet	Sophia Bou-Ghannam Kyungsook Kim David W. Grainger Teruo Okano
author_sort	Sophia Bou-Ghannam
title	3D cell sheet structure augments mesenchymal stem cell cytokine production
title_short	3D cell sheet structure augments mesenchymal stem cell cytokine production
title_full	3D cell sheet structure augments mesenchymal stem cell cytokine production
title_fullStr	3D cell sheet structure augments mesenchymal stem cell cytokine production
title_full_unstemmed	3D cell sheet structure augments mesenchymal stem cell cytokine production
title_sort	3d cell sheet structure augments mesenchymal stem cell cytokine production
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f2eaceb8ac6043cdb8dc2f9fddbe681f
work_keys_str_mv	AT sophiaboughannam 3dcellsheetstructureaugmentsmesenchymalstemcellcytokineproduction AT kyungsookkim 3dcellsheetstructureaugmentsmesenchymalstemcellcytokineproduction AT davidwgrainger 3dcellsheetstructureaugmentsmesenchymalstemcellcytokineproduction AT teruookano 3dcellsheetstructureaugmentsmesenchymalstemcellcytokineproduction
_version_	1718391286023585792

3D cell sheet structure augments mesenchymal stem cell cytokine production

Ejemplares similares