Change of the Shape of the Dural Sac in the Laminectomy Model at Different Stages of the Reparation in the Experiment

Epidural fibrosis is a dynamic pathological process that develops and progresses over time and is a polyetiological and multifactorial complication in the postoperative period during spinal surgery.Aim of the study. To study the reparative process and the formation of epidural fibrosis and its effec...

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Autores principales:	O. A. Goldberg, A. P. Zhivotenko, L. G. Samoilova, V. A. Sorokovikov, I. A. Shurygina
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2021
Materias:	laminectomy morphometry dural sac epidural fibrosis dura mater reparation experiment Science Q
Acceso en línea:	https://doaj.org/article/f2eb14dd0dac447aaceb1c39dbc0af68
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spelling	oai:doaj.org-article:f2eb14dd0dac447aaceb1c39dbc0af682021-11-23T06:14:45ZChange of the Shape of the Dural Sac in the Laminectomy Model at Different Stages of the Reparation in the Experiment2541-94202587-959610.29413/ABS.2020-5.6.34https://doaj.org/article/f2eb14dd0dac447aaceb1c39dbc0af682021-01-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/2525https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596Epidural fibrosis is a dynamic pathological process that develops and progresses over time and is a polyetiological and multifactorial complication in the postoperative period during spinal surgery.Aim of the study. To study the reparative process and the formation of epidural fibrosis and its effect on the shape of the dural sac in the area of laminectomy at different periods of wound healing.Materials and methods. To assess the dynamics of the formation of epidural fibrosis and the involvement of the dura mater in this process, laminectomy was performed at the level of the LVI vertebra in 35 male Wistar rats at the age of 4–5 months. The animals were divided into 5 periods of withdrawal from the experiment on days 3, 7, 14, 21, and 28. The histological material was studied by staining with hematoxylin and eosin. To assess the deformation of the dural sac, the ratio of the diameter to the length of the canal was assessed on histological preparations in the laminectomy zone, and these measurements were carried out in a group of intact animals at the level of the LVI vertebra (n = 7). Results. During the observation process, a change in the ratio of the sizes of the dural sac has been observed. In the period from days 3 to 14, the anterior-posterior size of the dural sac progressively decreases, in parallel, the transverse size increases, which leads to a change in its shape. On follow-up, some form correction occurs. It is possible that the change in the shape of the dural sac in terms of up to 14 days is due to edema, plasma saturation of tissues, as well as the growth of connective tissue. The subsequent decrease in edema and the restructuring of the newly formed connective tissue leads to a correction of the shape of the dural sac.Conclusion. In the work, the dynamics of morphological changes in the zone of formation of epidural fibrosis during laminectomy in the experiment was assessed. The dynamics of changes in the lumen of the dural sac was established. The early involvement of the dura mater in the pathological process, its interest during the entire observation period was noted. Also, histological preparations show the involvement of the nerve fibers of the cauda equina in the pathological process from the early stages after surgery: vacuolization and destruction of nerve fibers, adhesion of fibers to each other and to the dura mater, as well as activation of endotheliocytes in the vasa nervorum.O. A. GoldbergA. P. ZhivotenkoL. G. SamoilovaV. A. SorokovikovI. A. ShuryginaScientific Сentre for Family Health and Human Reproduction Problemsarticlelaminectomymorphometrydural sacepidural fibrosisdura materreparationexperimentScienceQRUActa Biomedica Scientifica, Vol 5, Iss 6, Pp 259-264 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	laminectomy morphometry dural sac epidural fibrosis dura mater reparation experiment Science Q
spellingShingle	laminectomy morphometry dural sac epidural fibrosis dura mater reparation experiment Science Q O. A. Goldberg A. P. Zhivotenko L. G. Samoilova V. A. Sorokovikov I. A. Shurygina Change of the Shape of the Dural Sac in the Laminectomy Model at Different Stages of the Reparation in the Experiment
description	Epidural fibrosis is a dynamic pathological process that develops and progresses over time and is a polyetiological and multifactorial complication in the postoperative period during spinal surgery.Aim of the study. To study the reparative process and the formation of epidural fibrosis and its effect on the shape of the dural sac in the area of laminectomy at different periods of wound healing.Materials and methods. To assess the dynamics of the formation of epidural fibrosis and the involvement of the dura mater in this process, laminectomy was performed at the level of the LVI vertebra in 35 male Wistar rats at the age of 4–5 months. The animals were divided into 5 periods of withdrawal from the experiment on days 3, 7, 14, 21, and 28. The histological material was studied by staining with hematoxylin and eosin. To assess the deformation of the dural sac, the ratio of the diameter to the length of the canal was assessed on histological preparations in the laminectomy zone, and these measurements were carried out in a group of intact animals at the level of the LVI vertebra (n = 7). Results. During the observation process, a change in the ratio of the sizes of the dural sac has been observed. In the period from days 3 to 14, the anterior-posterior size of the dural sac progressively decreases, in parallel, the transverse size increases, which leads to a change in its shape. On follow-up, some form correction occurs. It is possible that the change in the shape of the dural sac in terms of up to 14 days is due to edema, plasma saturation of tissues, as well as the growth of connective tissue. The subsequent decrease in edema and the restructuring of the newly formed connective tissue leads to a correction of the shape of the dural sac.Conclusion. In the work, the dynamics of morphological changes in the zone of formation of epidural fibrosis during laminectomy in the experiment was assessed. The dynamics of changes in the lumen of the dural sac was established. The early involvement of the dura mater in the pathological process, its interest during the entire observation period was noted. Also, histological preparations show the involvement of the nerve fibers of the cauda equina in the pathological process from the early stages after surgery: vacuolization and destruction of nerve fibers, adhesion of fibers to each other and to the dura mater, as well as activation of endotheliocytes in the vasa nervorum.
format	article
author	O. A. Goldberg A. P. Zhivotenko L. G. Samoilova V. A. Sorokovikov I. A. Shurygina
author_facet	O. A. Goldberg A. P. Zhivotenko L. G. Samoilova V. A. Sorokovikov I. A. Shurygina
author_sort	O. A. Goldberg
title	Change of the Shape of the Dural Sac in the Laminectomy Model at Different Stages of the Reparation in the Experiment
title_short	Change of the Shape of the Dural Sac in the Laminectomy Model at Different Stages of the Reparation in the Experiment
title_full	Change of the Shape of the Dural Sac in the Laminectomy Model at Different Stages of the Reparation in the Experiment
title_fullStr	Change of the Shape of the Dural Sac in the Laminectomy Model at Different Stages of the Reparation in the Experiment
title_full_unstemmed	Change of the Shape of the Dural Sac in the Laminectomy Model at Different Stages of the Reparation in the Experiment
title_sort	change of the shape of the dural sac in the laminectomy model at different stages of the reparation in the experiment
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2021
url	https://doaj.org/article/f2eb14dd0dac447aaceb1c39dbc0af68
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Change of the Shape of the Dural Sac in the Laminectomy Model at Different Stages of the Reparation in the Experiment

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