Embryonic developmental arrest in the annual killifish Austrolebias charrua: A proteomic approach to diapause III.

Diapause is a reversible developmental arrest faced by many organisms in harsh environments. Annual killifish present this mechanism in three possible stages of development. Killifish are freshwater teleosts from Africa and America that live in ephemeral ponds, which dry up in the dry season. The ju...

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Autores principales:	Cora Chalar, Graciela Clivio, Jimena Montagne, Alicia Costábile, Analía Lima, Nicolás G Papa, Nibia Berois, María José Arezo
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f2ec7d0219f342c8b74617ed12045d5f
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spelling	oai:doaj.org-article:f2ec7d0219f342c8b74617ed12045d5f2021-12-02T20:03:56ZEmbryonic developmental arrest in the annual killifish Austrolebias charrua: A proteomic approach to diapause III.1932-620310.1371/journal.pone.0251820https://doaj.org/article/f2ec7d0219f342c8b74617ed12045d5f2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0251820https://doaj.org/toc/1932-6203Diapause is a reversible developmental arrest faced by many organisms in harsh environments. Annual killifish present this mechanism in three possible stages of development. Killifish are freshwater teleosts from Africa and America that live in ephemeral ponds, which dry up in the dry season. The juvenile and adult populations die, and the embryos remain buried in the bottom mud until the next rainy season. Thus, species survival is entirely embryo-dependent, and they are perhaps the most remarkable extremophile organisms among vertebrates. The aim of the present study was to gather information about embryonic diapauses with the use of a "shotgun" proteomics approach in diapause III and prehatching Austrolebias charrua embryos. Our results provide insight into the molecular mechanisms of diapause III. Data are available via ProteomeXchange with identifier PXD025196. We detected a diapause-dependent change in a large group of proteins involved in different functions, such as metabolic pathways and stress tolerance, as well as proteins related to DNA repair and epigenetic modifications. Furthermore, we observed a diapause-associated switch in cytoskeletal proteins. This first glance into global protein expression differences between prehatching and diapause III could provide clues regarding the induction/maintenance of this developmental arrest in A. charrua embryos. There appears to be no single mechanism underlying diapause and the present data expand our knowledge of the molecular basis of diapause regulation. This information will be useful for future comparative approaches among different diapauses in annual killifish and/or other organisms that experience developmental arrest.Cora ChalarGraciela ClivioJimena MontagneAlicia CostábileAnalía LimaNicolás G PapaNibia BeroisMaría José ArezoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0251820 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cora Chalar Graciela Clivio Jimena Montagne Alicia Costábile Analía Lima Nicolás G Papa Nibia Berois María José Arezo Embryonic developmental arrest in the annual killifish Austrolebias charrua: A proteomic approach to diapause III.
description	Diapause is a reversible developmental arrest faced by many organisms in harsh environments. Annual killifish present this mechanism in three possible stages of development. Killifish are freshwater teleosts from Africa and America that live in ephemeral ponds, which dry up in the dry season. The juvenile and adult populations die, and the embryos remain buried in the bottom mud until the next rainy season. Thus, species survival is entirely embryo-dependent, and they are perhaps the most remarkable extremophile organisms among vertebrates. The aim of the present study was to gather information about embryonic diapauses with the use of a "shotgun" proteomics approach in diapause III and prehatching Austrolebias charrua embryos. Our results provide insight into the molecular mechanisms of diapause III. Data are available via ProteomeXchange with identifier PXD025196. We detected a diapause-dependent change in a large group of proteins involved in different functions, such as metabolic pathways and stress tolerance, as well as proteins related to DNA repair and epigenetic modifications. Furthermore, we observed a diapause-associated switch in cytoskeletal proteins. This first glance into global protein expression differences between prehatching and diapause III could provide clues regarding the induction/maintenance of this developmental arrest in A. charrua embryos. There appears to be no single mechanism underlying diapause and the present data expand our knowledge of the molecular basis of diapause regulation. This information will be useful for future comparative approaches among different diapauses in annual killifish and/or other organisms that experience developmental arrest.
format	article
author	Cora Chalar Graciela Clivio Jimena Montagne Alicia Costábile Analía Lima Nicolás G Papa Nibia Berois María José Arezo
author_facet	Cora Chalar Graciela Clivio Jimena Montagne Alicia Costábile Analía Lima Nicolás G Papa Nibia Berois María José Arezo
author_sort	Cora Chalar
title	Embryonic developmental arrest in the annual killifish Austrolebias charrua: A proteomic approach to diapause III.
title_short	Embryonic developmental arrest in the annual killifish Austrolebias charrua: A proteomic approach to diapause III.
title_full	Embryonic developmental arrest in the annual killifish Austrolebias charrua: A proteomic approach to diapause III.
title_fullStr	Embryonic developmental arrest in the annual killifish Austrolebias charrua: A proteomic approach to diapause III.
title_full_unstemmed	Embryonic developmental arrest in the annual killifish Austrolebias charrua: A proteomic approach to diapause III.
title_sort	embryonic developmental arrest in the annual killifish austrolebias charrua: a proteomic approach to diapause iii.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/f2ec7d0219f342c8b74617ed12045d5f
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Embryonic developmental arrest in the annual killifish Austrolebias charrua: A proteomic approach to diapause III.

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