Exploring the Structures and Functions of Macromolecular SLX4-Nuclease Complexes in Genome Stability

All organisms depend on the ability of cells to accurately duplicate and segregate DNA into progeny. However, DNA is frequently damaged by factors in the environment and from within cells. One of the most dangerous lesions is a DNA double-strand break. Unrepaired breaks are a major driving force for...

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Autores principales:	Brandon J. Payliss, Ayushi Patel, Anneka C. Sheppard, Haley D. M. Wyatt
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	SLX1-SLX4 MUS81-EME1 XPF-ERCC1 SLX4IP structure-selective endonuclease homologous recombination (HR) Genetics QH426-470
Acceso en línea:	https://doaj.org/article/f2f19c7c5e4f4094a492af45ec813ef0
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spelling	oai:doaj.org-article:f2f19c7c5e4f4094a492af45ec813ef02021-11-04T06:43:11ZExploring the Structures and Functions of Macromolecular SLX4-Nuclease Complexes in Genome Stability1664-802110.3389/fgene.2021.784167https://doaj.org/article/f2f19c7c5e4f4094a492af45ec813ef02021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fgene.2021.784167/fullhttps://doaj.org/toc/1664-8021All organisms depend on the ability of cells to accurately duplicate and segregate DNA into progeny. However, DNA is frequently damaged by factors in the environment and from within cells. One of the most dangerous lesions is a DNA double-strand break. Unrepaired breaks are a major driving force for genome instability. Cells contain sophisticated DNA repair networks to counteract the harmful effects of genotoxic agents, thus safeguarding genome integrity. Homologous recombination is a high-fidelity, template-dependent DNA repair pathway essential for the accurate repair of DNA nicks, gaps and double-strand breaks. Accurate homologous recombination depends on the ability of cells to remove branched DNA structures that form during repair, which is achieved through the opposing actions of helicases and structure-selective endonucleases. This review focuses on a structure-selective endonuclease called SLX1-SLX4 and the macromolecular endonuclease complexes that assemble on the SLX4 scaffold. First, we discuss recent developments that illuminate the structure and biochemical properties of this somewhat atypical structure-selective endonuclease. We then summarize the multifaceted roles that are fulfilled by human SLX1-SLX4 and its associated endonucleases in homologous recombination and genome stability. Finally, we discuss recent work on SLX4-binding proteins that may represent integral components of these macromolecular nuclease complexes, emphasizing the structure and function of a protein called SLX4IP.Brandon J. PaylissAyushi PatelAnneka C. SheppardHaley D. M. WyattHaley D. M. WyattFrontiers Media S.A.articleSLX1-SLX4MUS81-EME1XPF-ERCC1SLX4IPstructure-selective endonucleasehomologous recombination (HR)GeneticsQH426-470ENFrontiers in Genetics, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	SLX1-SLX4 MUS81-EME1 XPF-ERCC1 SLX4IP structure-selective endonuclease homologous recombination (HR) Genetics QH426-470
spellingShingle	SLX1-SLX4 MUS81-EME1 XPF-ERCC1 SLX4IP structure-selective endonuclease homologous recombination (HR) Genetics QH426-470 Brandon J. Payliss Ayushi Patel Anneka C. Sheppard Haley D. M. Wyatt Haley D. M. Wyatt Exploring the Structures and Functions of Macromolecular SLX4-Nuclease Complexes in Genome Stability
description	All organisms depend on the ability of cells to accurately duplicate and segregate DNA into progeny. However, DNA is frequently damaged by factors in the environment and from within cells. One of the most dangerous lesions is a DNA double-strand break. Unrepaired breaks are a major driving force for genome instability. Cells contain sophisticated DNA repair networks to counteract the harmful effects of genotoxic agents, thus safeguarding genome integrity. Homologous recombination is a high-fidelity, template-dependent DNA repair pathway essential for the accurate repair of DNA nicks, gaps and double-strand breaks. Accurate homologous recombination depends on the ability of cells to remove branched DNA structures that form during repair, which is achieved through the opposing actions of helicases and structure-selective endonucleases. This review focuses on a structure-selective endonuclease called SLX1-SLX4 and the macromolecular endonuclease complexes that assemble on the SLX4 scaffold. First, we discuss recent developments that illuminate the structure and biochemical properties of this somewhat atypical structure-selective endonuclease. We then summarize the multifaceted roles that are fulfilled by human SLX1-SLX4 and its associated endonucleases in homologous recombination and genome stability. Finally, we discuss recent work on SLX4-binding proteins that may represent integral components of these macromolecular nuclease complexes, emphasizing the structure and function of a protein called SLX4IP.
format	article
author	Brandon J. Payliss Ayushi Patel Anneka C. Sheppard Haley D. M. Wyatt Haley D. M. Wyatt
author_facet	Brandon J. Payliss Ayushi Patel Anneka C. Sheppard Haley D. M. Wyatt Haley D. M. Wyatt
author_sort	Brandon J. Payliss
title	Exploring the Structures and Functions of Macromolecular SLX4-Nuclease Complexes in Genome Stability
title_short	Exploring the Structures and Functions of Macromolecular SLX4-Nuclease Complexes in Genome Stability
title_full	Exploring the Structures and Functions of Macromolecular SLX4-Nuclease Complexes in Genome Stability
title_fullStr	Exploring the Structures and Functions of Macromolecular SLX4-Nuclease Complexes in Genome Stability
title_full_unstemmed	Exploring the Structures and Functions of Macromolecular SLX4-Nuclease Complexes in Genome Stability
title_sort	exploring the structures and functions of macromolecular slx4-nuclease complexes in genome stability
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/f2f19c7c5e4f4094a492af45ec813ef0
work_keys_str_mv	AT brandonjpayliss exploringthestructuresandfunctionsofmacromolecularslx4nucleasecomplexesingenomestability AT ayushipatel exploringthestructuresandfunctionsofmacromolecularslx4nucleasecomplexesingenomestability AT annekacsheppard exploringthestructuresandfunctionsofmacromolecularslx4nucleasecomplexesingenomestability AT haleydmwyatt exploringthestructuresandfunctionsofmacromolecularslx4nucleasecomplexesingenomestability AT haleydmwyatt exploringthestructuresandfunctionsofmacromolecularslx4nucleasecomplexesingenomestability
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Exploring the Structures and Functions of Macromolecular SLX4-Nuclease Complexes in Genome Stability

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