Cell responses without receptors and ligands, using nanosecond pulsed electric fields (nsPEFs)

Stephen J Beebe Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk VA, USAThe plasma membrane is a lipid bilayer that surrounds and shelters the living structural and metabolic systems within cells. That membrane is replete with transmembrane proteins with and without lig...

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Autor principal:	Beebe SJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/f2f2118f0d754beba68c5b6a2dd88707
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spelling	oai:doaj.org-article:f2f2118f0d754beba68c5b6a2dd887072021-12-02T03:03:57ZCell responses without receptors and ligands, using nanosecond pulsed electric fields (nsPEFs)1176-91141178-2013https://doaj.org/article/f2f2118f0d754beba68c5b6a2dd887072013-09-01T00:00:00Zhttp://www.dovepress.com/cell-responses-without-receptors-and-ligands-using-nanosecond-pulsed-e-a14233https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Stephen J Beebe Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk VA, USAThe plasma membrane is a lipid bilayer that surrounds and shelters the living structural and metabolic systems within cells. That membrane is replete with transmembrane proteins with and without ligand binding sites, oligosaccharides, and glycolipids on the cell exterior. Information transfer across this structure is closely controlled to maintain homeostasis and regulate cell responses to external stimuli. The plasma membrane is contiguous with the endoplasmic reticulum (ER) and nuclear membranes. A number of proteins form ER–mitochondria junctions, allowing interorganelle communications, especially for calcium transport. Transport mechanisms across these membranes include nongated channels or pores; single-gated channels for ion transport; carrier molecules for facilitated diffusion; and pumps for active transport of ions and macromolecules. During the activation of these transport systems, "pores" are formed through protein structures that transiently connect the intracellular and extracellular milieu. These pores are nanoscale structures with diameters of 0.2−4.0 nm. However, there can also be maligned movements of molecules across the plasma membranes. Staphylococcus aureus protein α-toxin and Streptococcus pyogenes protein streptolysin O both create pores that allow unsolicited molecular transfer across membranes that disrupts vital functions. Cytotoxic T-cells permeabilize the invading cell membranes with perforin, creating pores through which granzymes can induce apoptosis. These pores have a lumen of 5–30 nm with the majority at 13–20 nm.1Beebe SJDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 3401-3404 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Beebe SJ Cell responses without receptors and ligands, using nanosecond pulsed electric fields (nsPEFs)
description	Stephen J Beebe Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk VA, USAThe plasma membrane is a lipid bilayer that surrounds and shelters the living structural and metabolic systems within cells. That membrane is replete with transmembrane proteins with and without ligand binding sites, oligosaccharides, and glycolipids on the cell exterior. Information transfer across this structure is closely controlled to maintain homeostasis and regulate cell responses to external stimuli. The plasma membrane is contiguous with the endoplasmic reticulum (ER) and nuclear membranes. A number of proteins form ER–mitochondria junctions, allowing interorganelle communications, especially for calcium transport. Transport mechanisms across these membranes include nongated channels or pores; single-gated channels for ion transport; carrier molecules for facilitated diffusion; and pumps for active transport of ions and macromolecules. During the activation of these transport systems, "pores" are formed through protein structures that transiently connect the intracellular and extracellular milieu. These pores are nanoscale structures with diameters of 0.2−4.0 nm. However, there can also be maligned movements of molecules across the plasma membranes. Staphylococcus aureus protein α-toxin and Streptococcus pyogenes protein streptolysin O both create pores that allow unsolicited molecular transfer across membranes that disrupts vital functions. Cytotoxic T-cells permeabilize the invading cell membranes with perforin, creating pores through which granzymes can induce apoptosis. These pores have a lumen of 5–30 nm with the majority at 13–20 nm.1
format	article
author	Beebe SJ
author_facet	Beebe SJ
author_sort	Beebe SJ
title	Cell responses without receptors and ligands, using nanosecond pulsed electric fields (nsPEFs)
title_short	Cell responses without receptors and ligands, using nanosecond pulsed electric fields (nsPEFs)
title_full	Cell responses without receptors and ligands, using nanosecond pulsed electric fields (nsPEFs)
title_fullStr	Cell responses without receptors and ligands, using nanosecond pulsed electric fields (nsPEFs)
title_full_unstemmed	Cell responses without receptors and ligands, using nanosecond pulsed electric fields (nsPEFs)
title_sort	cell responses without receptors and ligands, using nanosecond pulsed electric fields (nspefs)
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/f2f2118f0d754beba68c5b6a2dd88707
work_keys_str_mv	AT beebesj cellresponseswithoutreceptorsandligandsusingnanosecondpulsedelectricfieldsnspefs
_version_	1718402016385957888

Cell responses without receptors and ligands, using nanosecond pulsed electric fields (nsPEFs)

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