Rehmannia glutinosa polysaccharide liposome as a novel strategy for stimulating an efficient immune response and their effects on dendritic cells

Yee Huang,1 Tao Qin,2 Yifan Huang,2 Zhenguang Liu,1 Ruonan Bo,1 Yuanliang Hu,1 Jiaguo Liu,1 Yi Wu,1 Deyun Wang1,2 1College of Veterinary Medicine, Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 2College of Animal Scien...

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Autores principales:	Huang Y, Qin T, Liu Z, Bo R, Hu Y, Liu J, Wu Y, Wang D
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Rehmannia glutinosa polysaccharide liposome adjuvant Controlled release Ovalbumin Dentritic cells Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/f2f5d1db39854f729ae6e7a66d5433d8
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spelling	oai:doaj.org-article:f2f5d1db39854f729ae6e7a66d5433d82021-12-02T05:02:11ZRehmannia glutinosa polysaccharide liposome as a novel strategy for stimulating an efficient immune response and their effects on dendritic cells1178-2013https://doaj.org/article/f2f5d1db39854f729ae6e7a66d5433d82016-12-01T00:00:00Zhttps://www.dovepress.com/rehmannia-glutinosa-polysaccharide-liposome-as-a-novel-strategy-for-st-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yee Huang,1 Tao Qin,2 Yifan Huang,2 Zhenguang Liu,1 Ruonan Bo,1 Yuanliang Hu,1 Jiaguo Liu,1 Yi Wu,1 Deyun Wang1,2 1College of Veterinary Medicine, Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 2College of Animal Science and Veterinary Medicine, Fujian Agriculture and Forestry University, Fuzhou, People’s Republic of China Abstract: Nanomedicine, the medical application of nanotechnology, promises a seemingly limitless range of applications from drug delivery to adjuvants and therapeutics. Our current research is focused on natural polymer-based liposome adjuvants. With the aim of inducing protective and long-lasting immunity, the immunological adjuvant activity of Rehmannia glutinosa polysaccharide liposome (RGPL) was investigated. In vivo, the splenic lymphocyte proliferation ratios and ovalbumin-specific immunoglobulin G titers of ovalbumin-RGPL-vaccinated mice were significantly upregulated. In draining lymph nodes, the expression of MHC II+CD11c+ and CD86+CD11c+ was increased by RGPL; in addition, the percentages of central memory cells (TCM) and effector memory cells (TEM) were also elevated. RGPL could effectively provide adequate antigen exposure in lymph nodes. In vitro, RGPL could promote dendritic cell maturation and enhance dendritic cell functions, such as the mixed lymphocyte reaction and antigen presentation. Overall, the results demonstrated that RGPL has the potential to act as an effective controlled release vaccine adjuvant. Keywords: Rehmannia glutinosa polysaccharide liposome, adjuvant, controlled release, ovalbumin, dendritic cellsHuang YQin THuang YLiu ZBo RHu YLiu JWu YWang DDove Medical PressarticleRehmannia glutinosa polysaccharide liposomeadjuvantControlled releaseOvalbuminDentritic cellsMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 6795-6808 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Rehmannia glutinosa polysaccharide liposome adjuvant Controlled release Ovalbumin Dentritic cells Medicine (General) R5-920
spellingShingle	Rehmannia glutinosa polysaccharide liposome adjuvant Controlled release Ovalbumin Dentritic cells Medicine (General) R5-920 Huang Y Qin T Huang Y Liu Z Bo R Hu Y Liu J Wu Y Wang D Rehmannia glutinosa polysaccharide liposome as a novel strategy for stimulating an efficient immune response and their effects on dendritic cells
description	Yee Huang,1 Tao Qin,2 Yifan Huang,2 Zhenguang Liu,1 Ruonan Bo,1 Yuanliang Hu,1 Jiaguo Liu,1 Yi Wu,1 Deyun Wang1,2 1College of Veterinary Medicine, Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 2College of Animal Science and Veterinary Medicine, Fujian Agriculture and Forestry University, Fuzhou, People’s Republic of China Abstract: Nanomedicine, the medical application of nanotechnology, promises a seemingly limitless range of applications from drug delivery to adjuvants and therapeutics. Our current research is focused on natural polymer-based liposome adjuvants. With the aim of inducing protective and long-lasting immunity, the immunological adjuvant activity of Rehmannia glutinosa polysaccharide liposome (RGPL) was investigated. In vivo, the splenic lymphocyte proliferation ratios and ovalbumin-specific immunoglobulin G titers of ovalbumin-RGPL-vaccinated mice were significantly upregulated. In draining lymph nodes, the expression of MHC II+CD11c+ and CD86+CD11c+ was increased by RGPL; in addition, the percentages of central memory cells (TCM) and effector memory cells (TEM) were also elevated. RGPL could effectively provide adequate antigen exposure in lymph nodes. In vitro, RGPL could promote dendritic cell maturation and enhance dendritic cell functions, such as the mixed lymphocyte reaction and antigen presentation. Overall, the results demonstrated that RGPL has the potential to act as an effective controlled release vaccine adjuvant. Keywords: Rehmannia glutinosa polysaccharide liposome, adjuvant, controlled release, ovalbumin, dendritic cells
format	article
author	Huang Y Qin T Huang Y Liu Z Bo R Hu Y Liu J Wu Y Wang D
author_facet	Huang Y Qin T Huang Y Liu Z Bo R Hu Y Liu J Wu Y Wang D
author_sort	Huang Y
title	Rehmannia glutinosa polysaccharide liposome as a novel strategy for stimulating an efficient immune response and their effects on dendritic cells
title_short	Rehmannia glutinosa polysaccharide liposome as a novel strategy for stimulating an efficient immune response and their effects on dendritic cells
title_full	Rehmannia glutinosa polysaccharide liposome as a novel strategy for stimulating an efficient immune response and their effects on dendritic cells
title_fullStr	Rehmannia glutinosa polysaccharide liposome as a novel strategy for stimulating an efficient immune response and their effects on dendritic cells
title_full_unstemmed	Rehmannia glutinosa polysaccharide liposome as a novel strategy for stimulating an efficient immune response and their effects on dendritic cells
title_sort	rehmannia glutinosa polysaccharide liposome as a novel strategy for stimulating an efficient immune response and their effects on dendritic cells
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/f2f5d1db39854f729ae6e7a66d5433d8
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Rehmannia glutinosa polysaccharide liposome as a novel strategy for stimulating an efficient immune response and their effects on dendritic cells

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