Identification of Nutritional Ingredients and Medicinal Components of Pueraria lobata and Its Varieties Using UPLC-MS/MS-Based Metabolomics

Pueraria lobata and its variety P. lobata var. thomsonii are both traditional Chinese medicines that have high nutritional and medical value; whereas another variety, P. lobata var. montana has low nutritional and medicinal...

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Autores principales:	Xiaohong Shang, Ding Huang, Ying Wang, Liang Xiao, Ruhong Ming, Wendan Zeng, Sheng Cao, Liuying Lu, Zhengdan Wu, Huabing Yan
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Pueraria lobata</i> UPLC-MS/MS metabolite profiling primary metabolites secondary metabolites Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/f2fb2cacfeec4c5db47815930e034dfe
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spelling	oai:doaj.org-article:f2fb2cacfeec4c5db47815930e034dfe2021-11-11T18:33:48ZIdentification of Nutritional Ingredients and Medicinal Components of <i>Pueraria lobata</i> and Its Varieties Using UPLC-MS/MS-Based Metabolomics10.3390/molecules262165871420-3049https://doaj.org/article/f2fb2cacfeec4c5db47815930e034dfe2021-10-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6587https://doaj.org/toc/1420-3049<i>Pueraria lobata</i> and its variety <i>P. lobata</i> var. thomsonii are both traditional Chinese medicines that have high nutritional and medical value; whereas another variety, <i>P</i>. <i>lobata</i> var. montana has low nutritional and medicinal value and can cause ecological disasters. The material basis of different nutritional and medicinal values, which are caused by metabolite differences among these varieties, remains to be further clarified. Here, we performed ultra performance liquid chromatography-tandem mass spectrometry based widely targeted metabolome analysis on <i>Pueraria lobata</i>, <i>P. lobata</i> var. thomsonii, and <i>P</i>. <i>lobata</i> var. montana. Among them, a total of 614 metabolites were identified, and distinguished from each other using orthogonal partial least squares discriminant analysis. Our results suggest that the nutritional differences between <i>P. lobata</i> and its varieties can be explained by variations in the abundance of amino acids, nucleotides, saccharides, and lipids; differences in flavonoids, isoflavones, phenolic acids, organic acids, and coumarins contents caused the differences in the medicinal quality of <i>P. lobata</i> and its varieties. Additionally, the key metabolites responsible for the classification of the three <i>Pueraria</i> varieties were identified. This study provides new insights into the underlying metabolic causes of nutritional and medicinal variation in <i>P. lobata</i> and its varieties.Xiaohong ShangDing HuangYing WangLiang XiaoRuhong MingWendan ZengSheng CaoLiuying LuZhengdan WuHuabing YanMDPI AGarticle<i>Pueraria lobata</i>UPLC-MS/MSmetabolite profilingprimary metabolitessecondary metabolitesOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6587, p 6587 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Pueraria lobata</i> UPLC-MS/MS metabolite profiling primary metabolites secondary metabolites Organic chemistry QD241-441
spellingShingle	<i>Pueraria lobata</i> UPLC-MS/MS metabolite profiling primary metabolites secondary metabolites Organic chemistry QD241-441 Xiaohong Shang Ding Huang Ying Wang Liang Xiao Ruhong Ming Wendan Zeng Sheng Cao Liuying Lu Zhengdan Wu Huabing Yan Identification of Nutritional Ingredients and Medicinal Components of <i>Pueraria lobata</i> and Its Varieties Using UPLC-MS/MS-Based Metabolomics
description	<i>Pueraria lobata</i> and its variety <i>P. lobata</i> var. thomsonii are both traditional Chinese medicines that have high nutritional and medical value; whereas another variety, <i>P</i>. <i>lobata</i> var. montana has low nutritional and medicinal value and can cause ecological disasters. The material basis of different nutritional and medicinal values, which are caused by metabolite differences among these varieties, remains to be further clarified. Here, we performed ultra performance liquid chromatography-tandem mass spectrometry based widely targeted metabolome analysis on <i>Pueraria lobata</i>, <i>P. lobata</i> var. thomsonii, and <i>P</i>. <i>lobata</i> var. montana. Among them, a total of 614 metabolites were identified, and distinguished from each other using orthogonal partial least squares discriminant analysis. Our results suggest that the nutritional differences between <i>P. lobata</i> and its varieties can be explained by variations in the abundance of amino acids, nucleotides, saccharides, and lipids; differences in flavonoids, isoflavones, phenolic acids, organic acids, and coumarins contents caused the differences in the medicinal quality of <i>P. lobata</i> and its varieties. Additionally, the key metabolites responsible for the classification of the three <i>Pueraria</i> varieties were identified. This study provides new insights into the underlying metabolic causes of nutritional and medicinal variation in <i>P. lobata</i> and its varieties.
format	article
author	Xiaohong Shang Ding Huang Ying Wang Liang Xiao Ruhong Ming Wendan Zeng Sheng Cao Liuying Lu Zhengdan Wu Huabing Yan
author_facet	Xiaohong Shang Ding Huang Ying Wang Liang Xiao Ruhong Ming Wendan Zeng Sheng Cao Liuying Lu Zhengdan Wu Huabing Yan
author_sort	Xiaohong Shang
title	Identification of Nutritional Ingredients and Medicinal Components of <i>Pueraria lobata</i> and Its Varieties Using UPLC-MS/MS-Based Metabolomics
title_short	Identification of Nutritional Ingredients and Medicinal Components of <i>Pueraria lobata</i> and Its Varieties Using UPLC-MS/MS-Based Metabolomics
title_full	Identification of Nutritional Ingredients and Medicinal Components of <i>Pueraria lobata</i> and Its Varieties Using UPLC-MS/MS-Based Metabolomics
title_fullStr	Identification of Nutritional Ingredients and Medicinal Components of <i>Pueraria lobata</i> and Its Varieties Using UPLC-MS/MS-Based Metabolomics
title_full_unstemmed	Identification of Nutritional Ingredients and Medicinal Components of <i>Pueraria lobata</i> and Its Varieties Using UPLC-MS/MS-Based Metabolomics
title_sort	identification of nutritional ingredients and medicinal components of <i>pueraria lobata</i> and its varieties using uplc-ms/ms-based metabolomics
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f2fb2cacfeec4c5db47815930e034dfe
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Identification of Nutritional Ingredients and Medicinal Components of <i>Pueraria lobata</i> and Its Varieties Using UPLC-MS/MS-Based Metabolomics

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